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Ctr9 (NM_009431) Mouse Tagged ORF Clone
CAT＃: MG225713
- TrueORF®
Ctr9 (tGFP-tagged) - Mouse Ctr9 Paf1/RNA polymerase II complex component homolog (S. cerevisiae) (Ctr9), (10ug)
ORF Plasmid: DDK
"NM_009431" in other vectors (4)
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CNY 10,550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA409336; mKIAA0155; Sh2bp1; Tsbp; Tsp |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225713 representing NM_009431
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCGGGGCTCCATCGAGATTCCCCTCCGGGATACTGACGAGGTCATTGAACTTGACTTCGATCAGT TACCGGAGGGAGACGAAGTCATCAGCATTCTGAAGCAGGAGCACACACAACTGCACATATGGATTGCCTT GGCGCTGGAATACTACAAGCAAGGGAAAACAGAAGAGTTTGTGAAGCTGCTGGAGGCAGCACGGATAGAT GGCAATTTGGACTATCGAGATCACGAGAAAGACCAGATGACTTGCTTGGATACATTGGCAGCCTACTATG TACAACAGGCTCGGAAAGAAAAGAACAAGGACAATAAGAAGGACCTTATTACGCAGGCAACTTTGTTATA TACAATGGCTGATAAAATTATTATGTATGATCAGAACCATTTGTTGGGAAGAGCCTGCTTCTGTCTGCTC GAGGGCGACAAAATGGACCAAGCTGACGCACAGTTTCACTTTGTACTTAACCAGTCTCCAAACAATATTC CAGCCCTTCTTGGGAAAGCTTGTATTTCATTCAACAAGAAGGATTACAGAGGAGCTCTGGCTTACTATAA AAAAGCATTGCGCACTAACCCGGGATGTCCAGCTGAAGTTCGTTTAGGAATGGGCCATTGCTTTGTCAAA CTTAACAAACTGGAAAAAGCTCGACTGGCATTCAGCAGAGCCCTGGAACTCAACTCGAAGTGTGTGGGAG CACTGGTTGGACTGGCTGTTCTAGAACTCAACAATAAAGAGGCTGATTCCATCAAAAATGGCGTGCAGCT TCTTTCCAGAGCCTATACTATTGATCCTAGCAACCCGATGGTACTGAACCATTTGGCAAACCACTTTTTC TTCAAGAAGGATTATAGTAAAGTGCAGCACCTGGCTCTCCACGCATTCCACAACACCGAGGTGGAGGCTA TGCAAGCAGAAAGCTGTTACCAGCTGGCTCGGTCCTTCCATGTTCAGGAAGATTATGACCAAGCCTTTCA GTACTACTATCAAGCCACACAGTTTGCTTCATCTTCCTTTGTCCTACCATTTTTTGGTTTGGGACAAATG TATATTTATCGAGGTGACAAAGAGAATGCATCTCAGTGCTTTGAGAAAGTTTTGAAAGCTTATCCGAACA ATTATGAAACTATGAAAATTCTTGGCTCTCTCTATGCTGCTTCAGAAGATCAGGAGAAACGAGACATAGC CAAGGGCCATTTGAAGAAGGTCACAGAACAGTATCCTGATGACGTGGAAGCTTGGATTGAGTTGGCCCAG ATCTTAGAACAGACTGACATACAGGGTGCCCTTTCAGCTTATGGAACAGCCACACGCATCCTGCAGGAGA AAGTGCAGGCCGATGTCCCTCCCGAGATTTTGAATAATGTTGGGGCCCTCCATTTTAGACTCGGAAACCT GGGGGAAGCAAAGAAATACTTCCTGGCCTCACTGGACCGCGCAAAGGCAGAGGCCGAGCATGATGAACAC TACTACAATGCCATCTCTGTCACCACATCCTACAATTTAGCCAGGCTCTATGAGGCCATGTGTGAATTTC ACGAAGCAGAGAAACTGTACAAAAACATTTTACGAGAGCATCCTAACTACGTTGATTGCTATCTACGCCT AGGAGCCATGGCCAGAGATAAAGGAAACTTCTATGAAGCTTCAGACTGGTTTAAGGAAGCCCTTCAGATC AATCAGGATCATCCGGATGCCTGGTCTTTGATTGGTAACCTCCATCTGGCAAAACAAGAATGGGGCCCAG GTCAGAAGAAGTTTGAGAGGATATTAAAACAGCCAGCCACACAGAGTGACACCTACTCTATGCTGGCCCT TGGCAATGTGTGGCTCCAGACTTTGCATCAGCCAACCCGAGACCGAGAAAAGGAAAAGCGCCATCAAGAC CGTGCCCTGGCCATCTACAAACAAGTCCTCAGAAATGATGCCAAGAACCTGTATGCTGCCAATGGCATAG GAGCTGTCTTGGCCCACAAAGGATACTTCCGTGAAGCTCGTGATGTTTTCGCCCAAGTGAGAGAAGCAAC AGCAGATATCAGTGATGTGTGGTTGAACCTAGCACACATCTACGTGGAGCAGAAGCAGTATATCAGCGCC GTTCAGATGTATGAGAACTGCCTCAGAAAGTTCTATAAGCACCAAAACACTGAGGTTGTGCTCTACCTGG CCCGCGCCCTCTTCAAGTGCGGCAAGCTGCAGGAGTGCAAGCAGACTCTGCTGAAGGCCAGACACGTGGC ACCCAGTGACACAGTTCTGATGTTTAATGTGGCCTTGGTCTTGCAAAGATTAGCTACCTCTGTCCTGAAA GATGAAAAAAGTAATCTGAAGGAAGTCCTTAATGCTGTGAAAGAGCTGGAGCTCGCACACAGGTACTTCA GTTACTTGAGCAAAGTTGGAGATAAAATGAGATTTGATTTGGCCCTTGCTGCCTCTGAAGCCAGGCAGTG CTCTGACTTACTGAGCCAGGCCCAGTACCATGTGGCCCGGGCACGCAAGCAGGATGAGGAAGAACGGGAG CTGCGGGCCAAGCAGGAGCAGGAAAAGGAGCTCTTAAGACAGAAGCTTCTCAAAGAGCAGGAAGAGAAAC GTCTCAGAGAAAAGGAAGAACAAAAGAAACTTTTGGAACAGCGTGCCCAGTATGTGGAGAAGACCAAAAA TATCCTTATGTTCACTGGCGAGACTGAAGCAACAAAGGAGAAGAAAAGAGGTGGAGGTGGAGGGCGGCGT TCCAAGAAAGGAGGCGAGTTTGATGAATTTGTCAACGATGACACCGACGATGACCTCCCTGTATCCAAAA AGAAGAAGAGAAGGAAGGGCAGTGGCAGTGAACAGGAAGGCGAAGAAGAGGAAGGTGGAGAGAGGAAGAA GAAGAGGAGGAGAAGACCTCCAAAGGGAGAAGAGGGATCTGAGGAGGAGGAGACAGAAAATGGCCCCAAA CCAAAGAAGCGCCGCCCACCGAGAGCAGAGAAAAAGAAGGCTCCCAAGCCAGAACGCCTGCCTCCTTCAA TGAAGGGAAAAATAAAATCCAAAGCCATTATATCATCAAGTGATGATTCTTCAGATGAGGATAAACTGAA AATTGCTGATGAAGGACATCCCAGGAATAGCAACAGCGATTCTGATGACGACGAGAGGCCCAACAGACGC GCCTCATCTGAGAGCGACTCAGATGACAACCAGAACAAGTCTGGCAGCGAGGCAGGCAGCCCTCGGAGGT CGGGTAGACAGGAGTCGGATGAGGATTCCGACAGTGACCAGCCGTCCAGAAAGAGAAGGCGCTCCGGCTC TGAGCAGTCGGACAATGAGTCTGTGCAGTCAGGGAGAAGCCCCTCCGGAGCTTCTGAGAACGAGAACGAT TCTCGCCCGGCTTCTCCAAGTGCAGAATCGGACCACGAATCAGAGCAGGGCTCTGACAACGAGGGCTCTG GCCAAGGCTCTGGAAATGAATCAGAACCAGAGGGCTCAAACAACGAGGCCTCAGACCGAGGCTCAGAACA TGGGTCAGATGATAGCGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225713 representing NM_009431
Red=Cloning site Green=Tags(s) MSRGSIEIPLRDTDEVIELDFDQLPEGDEVISILKQEHTQLHIWIALALEYYKQGKTEEFVKLLEAARID GNLDYRDHEKDQMTCLDTLAAYYVQQARKEKNKDNKKDLITQATLLYTMADKIIMYDQNHLLGRACFCLL EGDKMDQADAQFHFVLNQSPNNIPALLGKACISFNKKDYRGALAYYKKALRTNPGCPAEVRLGMGHCFVK LNKLEKARLAFSRALELNSKCVGALVGLAVLELNNKEADSIKNGVQLLSRAYTIDPSNPMVLNHLANHFF FKKDYSKVQHLALHAFHNTEVEAMQAESCYQLARSFHVQEDYDQAFQYYYQATQFASSSFVLPFFGLGQM YIYRGDKENASQCFEKVLKAYPNNYETMKILGSLYAASEDQEKRDIAKGHLKKVTEQYPDDVEAWIELAQ ILEQTDIQGALSAYGTATRILQEKVQADVPPEILNNVGALHFRLGNLGEAKKYFLASLDRAKAEAEHDEH YYNAISVTTSYNLARLYEAMCEFHEAEKLYKNILREHPNYVDCYLRLGAMARDKGNFYEASDWFKEALQI NQDHPDAWSLIGNLHLAKQEWGPGQKKFERILKQPATQSDTYSMLALGNVWLQTLHQPTRDREKEKRHQD RALAIYKQVLRNDAKNLYAANGIGAVLAHKGYFREARDVFAQVREATADISDVWLNLAHIYVEQKQYISA VQMYENCLRKFYKHQNTEVVLYLARALFKCGKLQECKQTLLKARHVAPSDTVLMFNVALVLQRLATSVLK DEKSNLKEVLNAVKELELAHRYFSYLSKVGDKMRFDLALAASEARQCSDLLSQAQYHVARARKQDEEERE LRAKQEQEKELLRQKLLKEQEEKRLREKEEQKKLLEQRAQYVEKTKNILMFTGETEATKEKKRGGGGGRR SKKGGEFDEFVNDDTDDDLPVSKKKKRRKGSGSEQEGEEEEGGERKKKRRRRPPKGEEGSEEEETENGPK PKKRRPPRAEKKKAPKPERLPPSMKGKIKSKAIISSSDDSSDEDKLKIADEGHPRNSNSDSDDDERPNRR ASSESDSDDNQNKSGSEAGSPRRSGRQESDEDSDSDQPSRKRRRSGSEQSDNESVQSGRSPSGASENEND SRPASPSAESDHESEQGSDNEGSGQGSGNESEPEGSNNEASDRGSEHGSDDSD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009431 |
| ORF Size | 3519 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009431.2, NP_033457.2 |
| RefSeq Size | 4317 bp |
| RefSeq ORF | 3522 bp |
| Locus ID | 22083 |
| UniProt ID | Q62018 |
| Gene Summary | Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3) and dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription (By similarity). Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of the SET1 complex. Involved in transcriptional regulation of IL6-responsive genes and in JAK-STAT pathway; may regulate DNA-association of STAT3.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223825 | Ctr9 (untagged) - Mouse Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (Ctr9), (10ug) |
CNY 15,110.00 |
|
| MR225713 | Ctr9 (Myc-DDK-tagged) - Mouse Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (Ctr9) |
CNY 8,168.00 |
|
| MR225713L3 | Lenti ORF clone of Ctr9 (Myc-DDK-tagged) - Mouse Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (Ctr9) |
CNY 11,500.00 |
|
| MR225713L4 | Lenti ORF clone of Ctr9 (mGFP-tagged) - Mouse Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (Ctr9) |
CNY 11,500.00 |
