United States
Germany
Japan
United Kingdom
China
Trpm8 (NM_134252) Mouse Tagged ORF Clone
CAT＃: MG225279
- TrueORF®
Trpm8 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily M member 8 (Trpm8), (10ug)
ORF Plasmid: DDK
"NM_134252" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CMR1; LTrpC-6; LTRPC6; Trp-p8; TRPP8 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225279 representing NM_134252
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTTCGAGGGAGCCAGGCTCAGCATGAGGAGCCGCAGAAATGGTACTATGGGCAGCACCCGGACCC TGTACTCCAGTGTATCTCGGAGCACAGACGTGTCCTACAGTGACAGTGATTTGGTGAATTTTATTCAGGC AAATTTTAAAAAACGAGAATGTGTCTTCTTTACCAGAGACTCCAAGGCCATGGAGAACATATGCAAGTGT GGTTATGCCCAGAGCCAGCACATCGAAGGCACCCAGATCAACCAAAATGAGAAGTGGAACTACAAAAAAC ATACCAAGGAGTTTCCAACAGACGCCTTCGGGGACATTCAGTTTGAGACTCTGGGGAAGAAAGGCAAGTA CTTACGCTTGTCCTGTGACACCGACTCTGAAACTCTCTACGAACTGCTGACCCAGCACTGGCACCTCAAA ACACCCAACCTGGTCATTTCAGTGACGGGTGGAGCCAAAAACTTTGCTTTGAAGCCACGCATGCGCAAGA TCTTCAGCAGGCTGATTTACATCGCACAGTCTAAAGGTGCGTGGATTCTCACTGGAGGCACTCACTACGG CCTGATGAAGTACATAGGCGAGGTGGTGAGAGACAACACCATCAGCAGGAACTCAGAAGAGAACATCGTG GCCATTGGCATCGCAGCATGGGGCATGGTCTCCAACAGGGACACCCTCATCAGGAGCTGTGATGATGAGG GACATTTTTCAGCTCAATACATCATGGATGACTTTACCAGAGACCCTCTATACATCCTGGACAACAACCA TACCCACCTGCTGCTTGTGGACAACGGTTGTCATGGACACCCCACAGTGGAAGCCAAGCTCCGGAATCAG CTGGAAAAGTACATCTCTGAGCGCACCAGTCAAGATTCCAACTATGGTGGTAAGATCCCCATCGTGTGTT TTGCCCAAGGAGGTGGAAGAGAGACTCTAAAAGCCATCAACACCTCTGTCAAAAGCAAGATCCCTTGTGT GGTGGTGGAAGGCTCGGGGCAGATTGCTGATGTGATCGCCAGCCTGGTGGAGGTGGAGGATGTTTTAACC TCTTCCATGGTCAAAGAGAAGCTGGTACGCTTTTTACCACGCACTGTGTCCCGGCTGCCTGAAGAGGAAA TTGAGAGCTGGATCAAATGGCTCAAAGAAATTCTTGAGAGTTCTCACCTACTCACAGTAATTAAGATGGA AGAGGCTGGAGATGAGATTGTGAGCAACGCCATTTCCTATGCGCTGTACAAAGCCTTCAGCACTAATGAG CAAGACAAGGACAACTGGAATGGACAGCTGAAGCTTCTGCTGGAGTGGAACCAGTTGGACCTTGCCAGTG ATGAGATCTTCACCAATGACCGCCGCTGGGAGTCTGCCGACCTTCAGGAGGTCATGTTCACGGCTCTCAT AAAGGACAGACCCAAGTTTGTCCGCCTCTTTCTGGAGAATGGCCTGAATCTGCAGAAGTTTCTCACCAAT GAAGTCCTCACAGAGCTCTTCTCCACCCACTTCAGCACCCTAGTGTACCGGAACCTGCAGATCGCCAAGA ACTCCTACAATGACGCACTCCTCACCTTTGTCTGGAAGTTGGTGGCAAACTTCCGTCGAAGCTTCTGGAA AGAGGACAGAAGCAGCAGGGAGGACTTGGATGTGGAACTCCATGATGCATCTCTCACCACCCGGCACCCG CTGCAAGCTCTCTTCATCTGGGCCATTCTTCAGAACAAGAAGGAACTCTCCAAGGTCATTTGGGAGCAGA CCAAAGGCTGTACTCTGGCAGCCTTGGGGGCCAGCAAGCTTCTGAAGACCCTGGCCAAAGTTAAGAATGA TATCAACGCTGCTGGGGAATCGGAGGAACTGGCCAATGAATATGAGACCCGAGCAGTGGAGTTGTTCACC GAGTGTTACAGCAATGATGAAGACTTGGCAGAACAGCTACTGGTCTACTCCTGCGAAGCCTGGGGTGGGA GCAACTGTCTGGAGCTGGCAGTGGAGGCTACAGATCAGCATTTCATCGCTCAGCCTGGGGTCCAGAATTT CCTTTCTAAGCAATGGTATGGAGAGATTTCCCGAGACACGAAGAACTGGAAGATTATCCTGTGTCTATTC ATTATCCCCTTAGTGGGCTGTGGCCTCGTATCATTTAGGAAGAAACCCATTGACAAGCACAAGAAGCTGC TGTGGTACTATGTGGCCTTCTTCACGTCGCCCTTCGTGGTCTTCTCCTGGAACGTGGTCTTCTACATCGC CTTCCTCCTGCTGTTTGCCTATGTGCTGCTCATGGACTTCCACTCAGTGCCACACACCCCCGAGCTGATC CTCTACGCCCTGGTCTTCGTCCTCTTCTGTGATGAAGTGAGGCAGTGGTACATGAACGGAGTGAATTATT TCACCGACCTATGGAACGTTATGGACACCCTGGGACTCTTCTACTTCATAGCGGGTATTGTATTCCGGCT CCACTCTTCTAATAAAAGCTCGTTGTACTCTGGGCGCGTCATTTTCTGTCTGGATTACATTATATTCACG CTAAGGCTCATCCACATTTTCACCGTCAGCAGGAACTTGGGACCCAAGATTATAATGCTGCAGCGGATGC TGATCGACGTTTTCTTCTTCCTGTTCCTCTTTGCTGTGTGGATGGTGGCCTTTGGCGTGGCCAGACAGGG GATCCTAAGGCAAAATGAACAGCGCTGGAGATGGATCTTCCGCTCTGTCATCTATGAGCCCTACCTGGCC ATGTTTGGCCAGGTTCCCAGTGACGTGGATAGTACCACATATGACTTCTCCCACTGTACCTTCTCGGGAA ATGAGTCCAAGCCACTGTGTGTGGAGCTGGATGAGCACAACCTGCCCCGCTTCCCTGAGTGGATCACCAT TCCGCTGGTGTGCATCTACATGCTCTCCACCAATATCCTTCTGGTCAACCTCCTGGTCGCCATGTTTGGC TACACGGTAGGCATTGTACAGGAGAACAACGACCAGGTCTGGAAATTCCAGCGGTACTTCCTGGTGCAGG AGTACTGCAACCGCCTAAACATCCCCTTCCCCTTCGTTGTCTTCGCTTATTTCTACATGGTGGTGAAGAA GTGTTTCAAATGCTGCTGTAAAGAGAAGAATATGGAGTCTAATGCCTGCTGTTTCAGAAATGAGGACAAT GAGACTTTGGCGTGGGAGGGTGTCATGAAGGAGAATTACCTTGTCAAGATCAACACGAAAGCCAACGACA ACTCAGAGGAGATGAGGCATCGGTTTAGACAACTGGACTCAAAGCTTAACGACCTCAAAAGTCTTCTGAA AGAGATTGCTAATAACATCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225279 representing NM_134252
Red=Cloning site Green=Tags(s) MSFEGARLSMRSRRNGTMGSTRTLYSSVSRSTDVSYSDSDLVNFIQANFKKRECVFFTRDSKAMENICKC GYAQSQHIEGTQINQNEKWNYKKHTKEFPTDAFGDIQFETLGKKGKYLRLSCDTDSETLYELLTQHWHLK TPNLVISVTGGAKNFALKPRMRKIFSRLIYIAQSKGAWILTGGTHYGLMKYIGEVVRDNTISRNSEENIV AIGIAAWGMVSNRDTLIRSCDDEGHFSAQYIMDDFTRDPLYILDNNHTHLLLVDNGCHGHPTVEAKLRNQ LEKYISERTSQDSNYGGKIPIVCFAQGGGRETLKAINTSVKSKIPCVVVEGSGQIADVIASLVEVEDVLT SSMVKEKLVRFLPRTVSRLPEEEIESWIKWLKEILESSHLLTVIKMEEAGDEIVSNAISYALYKAFSTNE QDKDNWNGQLKLLLEWNQLDLASDEIFTNDRRWESADLQEVMFTALIKDRPKFVRLFLENGLNLQKFLTN EVLTELFSTHFSTLVYRNLQIAKNSYNDALLTFVWKLVANFRRSFWKEDRSSREDLDVELHDASLTTRHP LQALFIWAILQNKKELSKVIWEQTKGCTLAALGASKLLKTLAKVKNDINAAGESEELANEYETRAVELFT ECYSNDEDLAEQLLVYSCEAWGGSNCLELAVEATDQHFIAQPGVQNFLSKQWYGEISRDTKNWKIILCLF IIPLVGCGLVSFRKKPIDKHKKLLWYYVAFFTSPFVVFSWNVVFYIAFLLLFAYVLLMDFHSVPHTPELI LYALVFVLFCDEVRQWYMNGVNYFTDLWNVMDTLGLFYFIAGIVFRLHSSNKSSLYSGRVIFCLDYIIFT LRLIHIFTVSRNLGPKIIMLQRMLIDVFFFLFLFAVWMVAFGVARQGILRQNEQRWRWIFRSVIYEPYLA MFGQVPSDVDSTTYDFSHCTFSGNESKPLCVELDEHNLPRFPEWITIPLVCIYMLSTNILLVNLLVAMFG YTVGIVQENNDQVWKFQRYFLVQEYCNRLNIPFPFVVFAYFYMVVKKCFKCCCKEKNMESNACCFRNEDN ETLAWEGVMKENYLVKINTKANDNSEEMRHRFRQLDSKLNDLKSLLKEIANNIK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_134252 |
| ORF Size | 3312 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_134252.2 |
| RefSeq Size | 3869 bp |
| RefSeq ORF | 3315 bp |
| Locus ID | 171382 |
| UniProt ID | Q8R4D5 |
| Gene Summary | Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223594 | Trpm8 (untagged) - Mouse transient receptor potential cation channel, subfamily M, member 8 (Trpm8), (10ug) |
CNY 14,160.00 |
|
| MR225279 | Trpm8 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 8 (Trpm8) |
CNY 7,696.00 |
|
| MR225279L3 | Lenti ORF clone of Trpm8 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 8 (Trpm8) |
CNY 10,260.00 |
|
| MR225279L4 | Lenti ORF clone of Trpm8 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 8 (Trpm8) |
CNY 10,260.00 |
