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Cyp1a1 (NM_001136059) Mouse Tagged ORF Clone
CAT＃: MG224897
- TrueORF®
Cyp1a1 (tGFP-tagged) - Mouse cytochrome P450 family 1 subfamily a polypeptide 1 (Cyp1a1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001136059" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AHH; AHRR; CP11; Cyp1a2; CYPIA1; P450-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224897 representing NM_001136059
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTTCCATGTATGGACTTCCAGCCTTCGTGTCAGCCACAGAGCTGCTCCTGGCTGTCACCGTATTCT GCCTTGGATTCTGGGTGGTCAGAGCCACAAGAACCTGGGTTCCCAAAGGCCTGAAGACTCCACCAGGGCC CTGGGGCTTGCCCTTCATTGGTCACATGCTGACTGTGGGGAAGAACCCACATCTGTCACTGACACGGCTG AGTCAGCAGTATGGGGACGTGCTGCAGATCCGCATCGGCTCCACTCCTGTGGTGGTGCTGAGCGGCCTGA ACACCATCAAGCAGGCCCTGGTGAGGCAGGGAGATGACTTCAAGGGCCGGCCAGACCTCTACAGCTTCAC ACTTATCACTAATGGCAAGAGCATGACTTTTAACCCAGACTCTGGACCCGTGTGGGCTGCCCGCCGGCGC CTGGCCCAGAATGCCCTGAAGAGCTTCTCCATAGCCTCGGACCCGACGTCAGCATCCTCTTGCTACTTGG AGGAGCACGTGAGCAAGGAGGCTAACTATCTCGTCAGCAAACTTCAGAAGGTGATGGCAGAGGTTGGCCA CTTTGACCCTTACAAGTATTTGGTCGTGTCAGTAGCCAATGTCATCTGTGCCATATGCTTTGGCCAACGT TATGACCATGATGACCAAGAGCTGCTCAGCATAGTCAATCTGAGCAATGAGTTTGGGGAGGTTACTGGCT CTGGATACCCAGCTGACTTCATTCCTGTCCTCCGTTACCTGCCTAACTCTTCCCTGGATGCCTTCAAGGA CTTGAATGATAAGTTCTACAGCTTCATGAAGAAGTTAATCAAAGAGCACTACAGGACATTTGAGAAGGGC CACATCCGGGACATCACAGACAGCCTCATTGAGCATTGTCAGGACAGGAAGCTGGACGAGAATGCCAATG TCCAGCTGTCAGATGATAAGGTCATCACGATTGTTTTGGACCTCTTTGGAGCTGGGTTTGACACAGTCAC AACTGCTATCTCGTGGAGCCTCATGTACCTGGTAACCAACCCTAGGGTACAGAGAAAGATCCAGGAGGAA CTAGACACAGTGATTGGCAGAGATCGGCAGCCCCGGCTTTCTGACAGACCTCAGCTGCCCTATCTGGAGG CCTTCATTCTGGAGACCTTCCGGCATTCATCCTTCGTCCCCTTCACCATCCCCCACAGCACCACAAGAGA TACAAGTCTGAATGGCTTCTATATCCCCAAGGGGTGCTGTGTCTTTGTGAACCAGTGGCAGGTTAACCAT GACCGGGAACTGTGGGGTGACCCAAACGAGTTCCGGCCTGAAAGGTTTCTCACCCCCAGCGGCACTCTGG ACAAGCGCTTGAGTGAGAAGGTCACTCTCTTTGGTTTGGGCAAGCGAAAGTGCATCGGAGAGACCATTGG CCGATCGGAGGTCTTTCTCTTCCTGGCCATCTTGCTGCAGCAAATAGAATTTAAGGTGTCTCCAGGGGAG AAGGTGGATATGACTCCTACCTATGGGCTGACTTTAAAACACGCCCGCTGTGAACACTTCCAAGTGCAGA TGCGGTCTTCTGGTCCTCAGCATCTTCAGGCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224897 representing NM_001136059
Red=Cloning site Green=Tags(s) MPSMYGLPAFVSATELLLAVTVFCLGFWVVRATRTWVPKGLKTPPGPWGLPFIGHMLTVGKNPHLSLTRL SQQYGDVLQIRIGSTPVVVLSGLNTIKQALVRQGDDFKGRPDLYSFTLITNGKSMTFNPDSGPVWAARRR LAQNALKSFSIASDPTSASSCYLEEHVSKEANYLVSKLQKVMAEVGHFDPYKYLVVSVANVICAICFGQR YDHDDQELLSIVNLSNEFGEVTGSGYPADFIPVLRYLPNSSLDAFKDLNDKFYSFMKKLIKEHYRTFEKG HIRDITDSLIEHCQDRKLDENANVQLSDDKVITIVLDLFGAGFDTVTTAISWSLMYLVTNPRVQRKIQEE LDTVIGRDRQPRLSDRPQLPYLEAFILETFRHSSFVPFTIPHSTTRDTSLNGFYIPKGCCVFVNQWQVNH DRELWGDPNEFRPERFLTPSGTLDKRLSEKVTLFGLGKRKCIGETIGRSEVFLFLAILLQQIEFKVSPGE KVDMTPTYGLTLKHARCEHFQVQMRSSGPQHLQA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001136059 |
| ORF Size | 1572 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001136059.2, NP_001129531.1 |
| RefSeq Size | 2619 bp |
| RefSeq ORF | 1575 bp |
| Locus ID | 13076 |
| UniProt ID | P00184 |
| Gene Summary | A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15alpha and C16alpha positions. Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation. Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system. Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer. May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217414 | Cyp1a1 (untagged) - Mouse cytochrome P450, family 1, subfamily a, polypeptide 1 (Cyp1a1), transcript variant 2, (10ug) |
CNY 6,750.00 |
|
| MR224897 | Cyp1a1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 1, subfamily a, polypeptide 1 (Cyp1a1), transcript variant 2 |
CNY 3,912.00 |
|
| MR224897L3 | Lenti ORF clone of Cyp1a1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 1, subfamily a, polypeptide 1 (Cyp1a1), transcript variant 2 |
CNY 6,460.00 |
|
| MR224897L4 | Lenti ORF clone of Cyp1a1 (mGFP-tagged) - Mouse cytochrome P450, family 1, subfamily a, polypeptide 1 (Cyp1a1), transcript variant 2 |
CNY 6,460.00 |
