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Rasgrf1 (NM_011245) Mouse Tagged ORF Clone
CAT＃: MG224849
- TrueORF®
Rasgrf1 (tGFP-tagged) - Mouse RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_011245" in other vectors (4)
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CNY 13,870.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI844718; CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190; P190-A; p190RhoGEF; Ras-GRF1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224849 representing NM_011245
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGGCGCGCCC ATGCAGAAAGCCATCCGGCTTAACGATGGCCACGTCGTGACCCTGGGACTGCTGGCACAGAAGGACGGCA CGCGCAAAGGCTACCTGAGCAAGAGGAGTGCGGACAACCCAAAATGGCAAACCAAGTGGTTTGCCCTGCT CCAGAACCTGCTTTTCTACTTCGAAAGCGACTCCAGCCCTCGGCCCTCGGGGCTCTACCTGCTCGAGGGA AGTATCTGTAAACGCGCGCCCTCCCCCAAGCGAGGGACCTCCTCCAAGGAGTCCGGCGAGAAACAGCAAC ATTACTTCACAGTGAACTTCTCCAATGACAGCCAGAAGACCTTGGAGTTGAGGACCGAGGATGCCAAGGA CTGTGACGAGTGGGTGGCTGCGATCGCTCGCGCCAGCTACAAGATACTGGCTACAGAACATGAGGCGCTT ATGCAGAAGTACTTACACCTGCTGCAGGTAGTGGAGACAGAGAAGACTGTGGCTAAGCAGCTGCGCCAGC AGCTTGAGGACGGTGAGGTTGAGATCGAGCGCCTGAAGACGGAGGTAACTATTACAAACCTGATCAAGGA CAATGATCGTATCCAATCCAGCAACAAAGCCGGTTCTGCTGATGATGAGGACAGTGACATCAAGAAAATT AAGAAGGTTCAGAGCTTCCTTCGGGGATGGCTGTGCCGGCGAAAGTGGAAGAACATCATCCAGGACTACA TCCGGTCTCCCCACGCGGACAGCATGCGCAAGAGGAACCAGGTGGTGTTCAGCATGCTGGAGGCCGAGGC TGAGTACGTCCAGCAGCTACACATCCTTGTCAACAACTTCCTGCGCCCACTGCGCATGGCCGCCAGCTCT AAGAAACCCCCTATAACACACGACGACGTCAGCAGTATCTTTCTGAACAGCGAGACCATCATGTTCCTGC ATCAGATCTTCTACCAAGGCCTGAAGGCCCGTATCTCCAGCTGGCCCACCCTGGTGCTGGCGGACCTGTT CGATATCCTGCTGCCCATGCTGAACATCTACCAGGAGTTCGTCCGCAACCACCAGTACAGCCTCCAGATC CTTGCTCACTGCAAGCAAAACCGGGACTTTGACAAGCTCCTCAAGCAGTACGAGGCCAAGCCAGACTGCG AGGAGCGCACACTGGAGACCTTCCTCACCTACCCAATGTTCCAGATCCCCAGGTACATCCTGACACTCCA TGAGCTGCTGGCCCACACACCTCATGAGCATGTGGAGCGCAACAGCCTGGACTATGCCAAATCCAAACTA GAGGAACTGTCCAGGATCATGCATGACGAAGTCAGTGAAACCGAGAACATCCGCAAAAACCTGGCCATTG AGCGTATGATCACGGAGGGCTGTGAGATCCTCCTGGACACCAGCCAGACCTTCGTGCGCCAAGGTTCCCT CATGCAGATGTCCTTGTCTGAAAAGAGCAAGTCCTCTAGGGGCCGCCTGGGGTCTCTGTCCACTAAGAAA GAGGGTGAGCGCCAGTGTTTCCTATTCTCCAAGCATCTCATTATCTGCACCAGAGGCTCTGGTGGGAAAC TGCACCTAACCAAGAATGGAGTCATATCCCTCATTGACTGCACCTTACTGGATGAACCCGAAAACCTGGA CGACGAAGCCAAAGGAGCCGGACCAGAGATAGAACACCTGGAGTTTAAGATCGGGGTGGAGCCAAAGGAC TCCCTACCCTTCACTGTCATCCTGGTAGCCTCAACCAGGCAGGAGAAGGCAGCATGGACCAGTGACATCA TCCAGTGCGTGGATAATATCCGCTGCAATGGGCTCATGATGAATGCCTTTGAAGAAAATTCCAAGGTCAC CGTGCCTCAGATGATCAAGTCTGACGCTTCCTTATATTGTGATGATGTTGACATTCGCTTCAGCAAAACC ATGAATTCTTGCAAAGTGCTTCAGATTCGCTATGCCAGCGTGGAGCGCCTGCTGGAGCGCCTGACTGATC TTCGCTTCCTGAGCATTGACTTTCTCAACACCTTCCTGCATTCCTACCGCGTCTTCACCAATGCTATGGT GGTCCTGGACAAGCTGATCAATATCTACAGAAAACCCATGAGTGCGATTCCTGCCAGGTCCCTGGAACTC TTGTTTTCCAGCAGCCACAACGCCAAGCTTCTGTACGGAGATGCCCCTAAGTCCCCTCGCGCCAGCCGCA AGTTCTCCTCGCCACCGCCCTTGGCCATCGGGACGTCATCCCCCAGCCGCCGTCGGAAGCTGTCCCTCAA CATCCCCATCATCACAGGCGGCAAGGCGCTGGAACTGGCTTCCCTTGGGTGCAGCTCCGATAGCTACGCC AACATACACTCACCCATATCTCCCTTCGGCAAAACCACACTGGACACTGGCAAGCTCTGTATGGCCAGCA GCTTACCCAAGACTCCAGAGGAGATTGATGTGCCTGCCACGATTCCAGAGAAGCCTGGGGAGCTCTCAGC CTCCAGGAAGCATAGCTCTGACGTCCTGAAAGAAGAGTCCGAGGATGACCAGAACCACAGTGATGAAGAC AACACAGAAGTGTCACCAGTTAAATCCCCACCAACACCAAAATCGTTCTTAAACAGAACCATTACAGAGT TTCCATTCTTTAACTACAACAATGGAATCCTGATGACCACGTGTCGTGATCTGGTGGACAATAACCGCAG CACTCTCTCAGCTACTTCTGCCTTTGCCATAGCGACTGCAGGAGCCAATGAAGGCCCCTCAAACAAGGAG GTGTTTCGAAGGATGTCTTTGGCCAACACAGGCTTTTCCTCTGACCAGAGAAATATCGACAAGGAGTTTG TGATCCGCAGAGCTGCCACCAATCGTGTGCTGAATGTGCTCCGCCACTGGGTCACCAAGCACACCCAGGA CTTTGATACCGACGATACGCTCAAATACAGGGTGATCTGCTTCCTGGAGGAGGTCATGCATGACCCAGAC CTTCTGACCCAAGAGCGAAAGGCAGCAGCCAACATCATCAGGACTCTGACCCTAGAAGAAACAACTGAAC AACATAGCATGCTGGAAGAAGTCATACTGATGACGGAGGGTGTGAAGACTGAGCCCTTCGAAAACCACCC AGCCCTGGAGATAGCAGAGCAGCTGACCCTGCTGGATCACCTTGTCTTCAAGAGTATTCCTTATGAAGAG TTCTTTGGCCAGGGCTGGATGAAGGCCGAGAAGTATGAAAGGACACCTTACATTATGAAAACCACCAAAC ACTTCAACCATGTCAGTAACTTCATTGCTTCAGAAATTATCCGAAACGAGGACATCAGCGCAAGGGCAAG CGCCATCGAGAAGTGGGTGGCTGTGGCCGACATATGTCGCTGCCTGCACAACTACAATGCTGTGCTGGAG ATCACCTCCTCCATCAACCGCAGCGCCATCTTCCGTCTCAAAAAGACATGGCTCAAAGTTTCTAAGCAGA CGAAATCTTTGCTTGACAAGCTCCAAAAGCTTGTGTCATCAGATGGCAGATTTAAGAACCTCAGAGAGTC TTTGAGAAATTGTGATCCACCCTGTGTCCCTTACCTGGGGATGTATCTCACCGACTTGGTGTTCATCGAG GAGGGGACACCCAATTACACAGAGGACGGCCTGGTCAACTTCTCCAAGATGAGAATGATCTCCCATATTA TCCGAGAGATTCGCCAGTTTCAGCAGACTACCTACAAAATCGACCCCCAGCCAAAGGTAATTCAGTACTT ACTGGATGAATCTTTCATGTTGGACGAAGAAAGCCTGTATGAGTCTTCGCTCCTAATCGAACCAAAACTC CCCACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224849 representing NM_011245
Red=Cloning site Green=Tags(s) MQKAIRLNDGHVVTLGLLAQKDGTRKGYLSKRSADNPKWQTKWFALLQNLLFYFESDSSPRPSGLYLLEG SICKRAPSPKRGTSSKESGEKQQHYFTVNFSNDSQKTLELRTEDAKDCDEWVAAIARASYKILATEHEAL MQKYLHLLQVVETEKTVAKQLRQQLEDGEVEIERLKTEVTITNLIKDNDRIQSSNKAGSADDEDSDIKKI KKVQSFLRGWLCRRKWKNIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASS KKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQI LAHCKQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKL EELSRIMHDEVSETENIRKNLAIERMITEGCEILLDTSQTFVRQGSLMQMSLSEKSKSSRGRLGSLSTKK EGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLDEPENLDDEAKGAGPEIEHLEFKIGVEPKD SLPFTVILVASTRQEKAAWTSDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKT MNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTNAMVVLDKLINIYRKPMSAIPARSLEL LFSSSHNAKLLYGDAPKSPRASRKFSSPPPLAIGTSSPSRRRKLSLNIPIITGGKALELASLGCSSDSYA NIHSPISPFGKTTLDTGKLCMASSLPKTPEEIDVPATIPEKPGELSASRKHSSDVLKEESEDDQNHSDED NTEVSPVKSPPTPKSFLNRTITEFPFFNYNNGILMTTCRDLVDNNRSTLSATSAFAIATAGANEGPSNKE VFRRMSLANTGFSSDQRNIDKEFVIRRAATNRVLNVLRHWVTKHTQDFDTDDTLKYRVICFLEEVMHDPD LLTQERKAAANIIRTLTLEETTEQHSMLEEVILMTEGVKTEPFENHPALEIAEQLTLLDHLVFKSIPYEE FFGQGWMKAEKYERTPYIMKTTKHFNHVSNFIASEIIRNEDISARASAIEKWVAVADICRCLHNYNAVLE ITSSINRSAIFRLKKTWLKVSKQTKSLLDKLQKLVSSDGRFKNLRESLRNCDPPCVPYLGMYLTDLVFIE EGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTTYKIDPQPKVIQYLLDESFMLDEESLYESSLLIEPKL PT TRTRPLE - GFP Tag - V |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011245 |
| ORF Size | 3786 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011245.2, NP_035375.1 |
| RefSeq Size | 4243 bp |
| RefSeq ORF | 3789 bp |
| Locus ID | 19417 |
| UniProt ID | P27671 |
| Gene Summary | Promotes the exchange of Ras-bound GDP by GTP.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224106 | Rasgrf1 (untagged) - Mouse RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 1, (10ug) |
CNY 16,340.00 |
|
| MR224849 | Rasgrf1 (Myc-DDK-tagged) - Mouse RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 1 |
CNY 13,192.00 |
|
| MR224849L3 | Lenti ORF clone of Rasgrf1 (Myc-DDK-tagged) - Mouse RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 1 |
CNY 14,540.00 |
|
| MR224849L4 | Lenti ORF clone of Rasgrf1 (mGFP-tagged) - Mouse RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 1 |
CNY 14,540.00 |
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