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Arhgef7 (NM_017402) Mouse Tagged ORF Clone
CAT＃: MG223397
- TrueORF®
Arhgef7 (tGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF7) (Arhgef7) transcript variant 3, (10ug)
ORF Plasmid: DDK
"NM_017402" in other vectors (4)
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CNY 8,816.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | betaPix; betaPix-b; betaPix-c; Cool; cool-1; mKIAA0142; p85Cool1; p85SPR; Pak3bp; PIX |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223397 representing NM_017402
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGATAACACCAACAGCCAACTGGTAGTACGAGCCAAGTTTAACTTCCAGCAGACCAATGAAGATG AACTCTCCTTCTCAAAGGGTGATGTCATCCATGTCACACGAGTGGAGGAAGGAGGCTGGTGGGAGGGCAC ACACAATGGCAGGACCGGCTGGTTCCCCAGCAACTACGTTCGAGAGATCAAGCCAAGTGAGAAGCCCGTG TCACCCAAATCAGGGACCTTGAAGAGCCCTCCCAAAGGGTTCGATACGACTGCCATCAACAAGAGCTATT ACAACGTGGTGCTACAGAACATCCTGGAAACAGAGCATGAGTATTCCAAGGAGCTGCAGTCTGTGCTGTC CACCTACCTGCGGCCACTGCAGACCAGTGACAAGTTGAGTTCAGCAAACACTTCATATTTAATGGGAAAT CTAGAGGAAATATCTTCCTTCCAGCAAGTGCTTGTACAGTCCCTAGAAGAATGCACGAAGTCTCCTGAAG CCCAACAGAGAGTTGGTGGCTGCTTCCTGAGCCTGATGCCGCAGATGAGGACCCTGTACCTCGCTTACTG TGCCAACCACCCATCTGCTGTGAGCGTCCTCACAGAGCACAGTGAGGACCTAGGAGAGTTCATGGAAACA AAAGGTGCCAGCAGCCCTGGGATCCTGGTGCTGACCACCGGCCTGAGCAAGCCCTTCATGCGCCTGGACA AGTACCCCACACTGCTAAAGGAGCTGGAGAGACACATGGAGGATTATCATCCTGATAGACAAGATATTCA GAAGTCTATGACGGCCTTCAAAAACCTTTCAGCTCAGTGTCAAGAAGTTCGCAAGAGGAAGGAGCTGGAG CTGCAGATCCTGACGGAGCCCATCAGGAGCTGGGAGGGGGATGACATAAAGACCCTGGGCAGTGTCACAT ACATGTCCCAAGTCACCATTCAGTGTGCGGGAAGCGAGGAGAAGAATGAGAGATACCTCCTGCTCTTCCC AAACCTTCTGCTCATGTTGTCTGCAAGTCCCAGGATGAGTGGTTTCATCTATCAGGGGAAGCTGCCAACA ACAGGAATGACAATCACAAAGCTTGAGGACAGTGAAAACCATAGGAATGCATTTGAGATATCAGGGAGCA TGATCGAGCGGATTCTGGTGTCCTGCACCAGCCAGCAGGACTTACACGAGTGGGTGGAACACCTGCAGAA GCAGACGAAGGTCACATCTGTGAGCAACCCCACCATCAAACCCCACTCGGTGCCATCACACACACTTCCT TCCCATCCTCTCACTCCATCCAGCAAACACGCGGACAGCAAGCCCGTGGCACTGACGCCTGCGTACCACA CACTCCCCCACCCCTCTCACCATGGCACCCCACACACCACCATCAGCTGGGGACCCCTGGAGCCTCCGAA GACCCCCAAGCCTTGGAGCCTGAGTTGCCTGCGGCCTGCACCTCCCCTCCGGCCCTCAGCTGCTCTCTGC TACAAGGAGGATCTCAGTAAGAGCCCCAAGACCATGAAAAAGCTGCTGCCGAAGCGCAAGCCCGAGCGGA AGCCTTCGGACGAGGAGTTCGCTGTGCGCAAGAGCACAGCGGCGCTGGAAGAAGACGCTCAGATCCTGAA GGTTATCGAAGCTTATTGCACAAGTGCAAAGACGCGCCAGACCCTGAACTCAAGTTCACGCAAAGAATCT GCTCCACAAGTGTTGCTTCCAGAAGAAGAAAAAATTATAGTCGAAGAAACCAAGAGCAATGGGCAGACAG TGATAGAGGAAAAGAGCCTCGTGGATACGGTGTATGCGTTAAAGGATGAAGTCCAGGAGTTAAGACAGGA TAACAAGAAGATGAAGAAGTCCTTAGAGGAGGAACAGAGGGCCCGCAAGGACTTAGAGAAGCTGGTGAGG AAGGTTCTAAAGAATATGAACGACCCTGCCTGGGATGAGACCAATCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223397 representing NM_017402
Red=Cloning site Green=Tags(s) MTDNTNSQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTHNGRTGWFPSNYVREIKPSEKPV SPKSGTLKSPPKGFDTTAINKSYYNVVLQNILETEHEYSKELQSVLSTYLRPLQTSDKLSSANTSYLMGN LEEISSFQQVLVQSLEECTKSPEAQQRVGGCFLSLMPQMRTLYLAYCANHPSAVSVLTEHSEDLGEFMET KGASSPGILVLTTGLSKPFMRLDKYPTLLKELERHMEDYHPDRQDIQKSMTAFKNLSAQCQEVRKRKELE LQILTEPIRSWEGDDIKTLGSVTYMSQVTIQCAGSEEKNERYLLLFPNLLLMLSASPRMSGFIYQGKLPT TGMTITKLEDSENHRNAFEISGSMIERILVSCTSQQDLHEWVEHLQKQTKVTSVSNPTIKPHSVPSHTLP SHPLTPSSKHADSKPVALTPAYHTLPHPSHHGTPHTTISWGPLEPPKTPKPWSLSCLRPAPPLRPSAALC YKEDLSKSPKTMKKLLPKRKPERKPSDEEFAVRKSTAALEEDAQILKVIEAYCTSAKTRQTLNSSSRKES APQVLLPEEEKIIVEETKSNGQTVIEEKSLVDTVYALKDEVQELRQDNKKMKKSLEEEQRARKDLEKLVR KVLKNMNDPAWDETNL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017402 |
| ORF Size | 1938 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017402.5 |
| RefSeq Size | 4488 bp |
| RefSeq ORF | 1941 bp |
| Locus ID | 54126 |
| UniProt ID | Q9ES28 |
| Gene Summary | Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. May function as a positive regulator of apoptosis. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Microtubule nucleation in mouse bone marrow-derived mast cells is regulated by the concerted action of GIT1/βPIX proteins and calcium.
,null,
Journal of immunology (Baltimore, Md. : 1950)
,PubMed ID 25821222
[Arhgef7]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220088 | Arhgef7 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF7) (Arhgef7), transcript variant 3, (10ug) |
CNY 8,360.00 |
|
| MR223397 | Arhgef7 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF7) (Arhgef7), transcript variant 3 |
CNY 7,216.00 |
|
| MR223397L3 | Lenti ORF clone of Arhgef7 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF7) (Arhgef7), transcript variant 3 |
CNY 8,360.00 |
|
| MR223397L4 | Lenti ORF clone of Arhgef7 (mGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF7) (Arhgef7), transcript variant 3 |
CNY 8,360.00 |
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