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Jph3 (NM_020605) Mouse Tagged ORF Clone
CAT＃: MG223391
- TrueORF®
Jph3 (tGFP-tagged) - Mouse junctophilin 3 (Jph3), (10ug)
ORF Plasmid: DDK
"NM_020605" in other vectors (4)
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CNY 7,130.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | JP-3; Jp3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223391 representing NM_020605
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGTGGGGGTAGGTTTAATTTTGACGATGGAGGGTCCTACTGCGGAGGCTGGGAGGACGGCAAGG CGCACGGCCATGGCGTCTGCACCGGCCCGAAGGGCCAGGGCGAATACACCGGCTCGTGGAGCCACGGCTT TGAGGTGCTGGGCGTCTACACCTGGCCAAGCGGCAACACGTACCAAGGCACCTGGGCGCAGGGCAAGCGC CACGGCATCGGCCTGGAGAGCAAGGGGAAGTGGGTGTACAAGGGCGAGTGGACGCACGGATTTAAGGGGC GCTACGGGGTGCGGGAGTGCACGGGCAACGGAGCCAAATACGAAGGGACCTGGAGCAACGGACTGCAGGA TGGCTACGGCACGGAGACCTACTCGGATGGAGGAACGTATCAGGGCCAATGGGTTGGTGGCATGCGCCAG GGCTACGGTGTGCGGCAGAGTGTCCCTTATGGCATGGCCGCAGTCATCCGTTCGCCCCTGAGGACCTCCA TCAACTCCCTGCGCAGCGAGCACACCAACGGGGCGGCACTGCACCCGGACGCCTCCCCGGCAGTGGCCGG CAGTCCGGCCGTGTCCCGTGGTGGCTTCGTGCTGGTGGCCCACAGTGACTCGGAGATCCTCAAGAGCAAG AAGAAGGGACTGTTCCGGCGCTCGCTGCTGAGCGGCCTGAAGCTACGCAAGTCTGAGTCCAAGAGCAGCC TGGCCAGCCAGCGCAGCAAGCAGAGCTCCTTCCGCAGCGAGGCCGGAATGAGCACCGTGAGCTCCACGGC CAGCGACATCCACTCCACCATCAGCCTGGGCGAGGCCGAGGCTGAGCTGGCGGTCATTGAGGACGACATC GACGCCACTACCACCGAGACCTACGTGGGTGAATGGAAGAACGACAAACGCTCGGGCTTCGGTGTGAGCC AGCGCTCGGACGGGCTTAAGTATGAGGGCGAGTGGGTCAGCAACCGCCGGCATGGGTACGGGTGCATGAC TTTCCCCGACGGCACCAAGGAGGAGGGGAAGTACAAGCAGAACGTGCTCGTGAGCGGCAAACGCAAGAAT CTCATCCCGCTGCGTGCCAGCAAGATCCGGGAGAAGGTGGACCGGGCCGTGGAGGCAGCCGAGAGGGCGG CCACCATCGCCAAGCAGAAAGCCGAGATCGCTGCGTCCAGGACCTCCCACTCACGGGCCAAGGCTGAGGC AGCACTTACTGCGGCTCAGAAAGCCCAGGAGGAAGCGCGGATCGCCAGGATCACTGCCAAAGAGTTCTCT CCCTCCTTCCAGCACAGGGAAAACGGGCTTGAGTACCAGAGGCCAAAGCACCAGATGTCCTGCGATGATA TCGAGGTGCTCTCCACCGGGACACCCCTACAGCAGGAGAGCCCCGAGCTGTACCGAAAGGGCACCACCCC TTCTGATCTGACCCCTGACGACAGTCCCCTGCAGAGCTTCCCCGCCAGCCCCACATCTACCCCTCCGCCA GCTCCTGCCTCCAGGACCAAGATGGCCCACTTCTCCAGGCAGGTCTCCGTGGACGAGGAGCGAAGCGGGG ACATCCAGATGCTCCTCGAGGGCCGCGGAGGGGACTATGCCCGCAACAGCTGGGGCGAGGAGAAGGCTGG GGCCTCTAGGGGTATCCGCAGCGGTGCCCTACGCAGCGGCCAGCCCACTGAGGACTTCCGCACCCGGGGC TCAGGCCACAAGCAGCCTGGGAACCCCAAGCCCCGGGAGCGGCGGACGGAGTCCCCCACCACGTTCTCAT GGACTTCCCACCACCGGGCAGGCAATCCCTGTTCTGGGGGCCCCAAGCTGCTAGAGCCAGATGAGGAGCA GCTGAGCAACTACAAGCTGGAGATGAAGCCCTTACTGAGGATGGATGCCTGTCCACAGGATACGCACCCA CAGAGACGGCGTCATAGCCGGGGTGCTGGGGGGGACAGGGGCTTCGGGCTACAGAGACTGAGGTCCAAGT CCCAAAACAAGGAGAACCTTAGGCCAGCCTCCTCTGCAGAACCCACGGTGCAGAAACTCGAGAGCCTGCG GCTGGGGGACCGGCCCGAGCCCCGTCTGCTGCGTTGGGACCTGACCTTCTCCCCACCCCAGAAGTCCCTG CCTGTTGCACTCGAGTCTGACGAGGAGACTGGAGATGAGCTCAAGTCCAGCACGGGCTCAGCTCCCATCT TGGTCGTCATGGTGATCCTGCTCAACATTGGAGTCGCCATTTTGTTTATTAACTTTTTCATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223391 representing NM_020605
Red=Cloning site Green=Tags(s) MSSGGRFNFDDGGSYCGGWEDGKAHGHGVCTGPKGQGEYTGSWSHGFEVLGVYTWPSGNTYQGTWAQGKR HGIGLESKGKWVYKGEWTHGFKGRYGVRECTGNGAKYEGTWSNGLQDGYGTETYSDGGTYQGQWVGGMRQ GYGVRQSVPYGMAAVIRSPLRTSINSLRSEHTNGAALHPDASPAVAGSPAVSRGGFVLVAHSDSEILKSK KKGLFRRSLLSGLKLRKSESKSSLASQRSKQSSFRSEAGMSTVSSTASDIHSTISLGEAEAELAVIEDDI DATTTETYVGEWKNDKRSGFGVSQRSDGLKYEGEWVSNRRHGYGCMTFPDGTKEEGKYKQNVLVSGKRKN LIPLRASKIREKVDRAVEAAERAATIAKQKAEIAASRTSHSRAKAEAALTAAQKAQEEARIARITAKEFS PSFQHRENGLEYQRPKHQMSCDDIEVLSTGTPLQQESPELYRKGTTPSDLTPDDSPLQSFPASPTSTPPP APASRTKMAHFSRQVSVDEERSGDIQMLLEGRGGDYARNSWGEEKAGASRGIRSGALRSGQPTEDFRTRG SGHKQPGNPKPRERRTESPTTFSWTSHHRAGNPCSGGPKLLEPDEEQLSNYKLEMKPLLRMDACPQDTHP QRRRHSRGAGGDRGFGLQRLRSKSQNKENLRPASSAEPTVQKLESLRLGDRPEPRLLRWDLTFSPPQKSL PVALESDEETGDELKSSTGSAPILVVMVILLNIGVAILFINFFI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020605 |
| ORF Size | 2232 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020605.3, NP_065630.1 |
| RefSeq Size | 3903 bp |
| RefSeq ORF | 2235 bp |
| Locus ID | 57340 |
| UniProt ID | Q9ET77 |
| Gene Summary | Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. JPH3 is brain-specific and appears to have an active role in certain neurons involved in motor coordination and memory.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221235 | Jph3 (untagged) - Mouse junctophilin 3 (Jph3), (10ug) |
CNY 9,600.00 |
|
| MR223391 | Jph3 (Myc-DDK-tagged) - Mouse junctophilin 3 (Jph3) |
CNY 5,456.00 |
|
| MR223391L3 | Lenti ORF clone of Jph3 (Myc-DDK-tagged) - Mouse junctophilin 3 (Jph3) |
CNY 8,360.00 |
|
| MR223391L4 | Lenti ORF clone of Jph3 (mGFP-tagged) - Mouse junctophilin 3 (Jph3) |
CNY 8,360.00 |
