Tle3 (NM_009389) Mouse Tagged ORF Clone

CAT＃: MG223072

TrueORF^®

Tle3 (tGFP-tagged) - Mouse transducin-like enhancer of split 3 homolog of Drosophila E(spl) (Tle3) transcript variant 2, (10ug)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_009389" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 8,460.00

货期*

3周

规格

Product images

经常一起买 (4)

PS100010

pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug

CNY 6,080.00

TA150041

Mouse monoclonal turboGFP antibody, clone OTI2H8

CNY 1,000.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2610103N05Rik; ESG; Grg3a; Grg3b; mKIAA1547
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG223072 representing NM_009389 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATCCGCAAGGCAGACATCCGGCACCCCATCAACCCGGGCAGCCGGGATTTAAATTCACTGTGGCCG AGTCCTGTGACAGGATCAAAGACGAATTCCAGTTCCTGCAAGCTCAGTATCACAGCCTCAAAGTGGAGTA TGACAAGCTGGCTAACGAGAAGACGGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTATGGC TTGAATATTGAAATGCACAAGCAGACAGAGATTGCGAAGAGACTGAACACAATCCTAGCCCAGATCATGC CTTTTTTGTCACAGGAGCATCAGCAGCAAGTGGCGCAGGCTGTGGAACGCGCCAAGCAGGTCACCATGAC GGAGTTGAACGCCATCATCGGGCAGCAGCAACTCCAGGCCCAGCACCTCTCCCATGCCACGCATGGTCCC CCGGTCCAGCTGCCACCCCACCCGTCAGGCCTCCAGCCTCCTGGGATTCCCCCAGTGACAGGAAGCAGCT CTGGGTTGCTGGCACTTGGTGCCCTGGGAAGTCAAGCTCACTTGGCGGTGAAGGATGAGAAGAACCACCA TGAACTGGATCACAGAGAGAGAGAGTCCAGCACGAACAATTCCGTGTCACCCTCTGAAAGCCTCCGGGCC AGTGAGAAGCACCGGGGCTCTGCAGACTACAGCATGGAAGCCAAGAAGCGGAAGGCGGAAGAGAAAGACA GCCTCAGCAGATACGATAGCGATGGGGACAAGAGTGACGACCTGGTGGTGGATGTCTCTAATGAGGACCC AGCAACACCCCGGGTGAGCCCAGCACACTCCCCTCCTGAAAATGGGCTGGACAAAGCCCGTGGTCTGAAG AAAGATGCCCCCACCAGCCCAGCCTCCGTGGCTTCCTCCAGCAGCACACCTTCCTCCAAGACCAAAGACC TTGGTCATAATGACAAATCTTCCACACCTGGGCTCAAGTCCAACACACCAACGCCAAGAAATGATGCCCC AACTCCAGGCACCAGCACCACCCCGGGGCTCCGGTCAATGCCGGGCAAACCTCCAGGCATGGACCCGATA GGTATAATGGCCTCGGCCCTGCGAACACCCATCACCCTCACCAGCTCCTATCCAGCACCCTTTGCCATGA TGAGCCACCACGAGATGAATGGCTCCCTCACCAGCCCAAGCGCCTATGCTGGCCTACACAACATCCCATC CCAGATGAGCGCCGCCGCAGCCGCTGCAGCCGCCGCCTATGGCCGATCGCCAATGGTGAGCTTTGGAGCT GTTGGTTTTGACCCTCACCCCCCAATGAGGGCCACAGGCCTGCCTTCCAGTCTCGCCTCCATTCCTGGAG GGAAACCGGCATACTCCTTCCATGTGAGTGCTGATGGGCAGATGCAACCTGTGCCCTTCCCCCATGATGC ACTAGCAGGCCCTGGCATTCCCAGGCATGCCCGGCAGATCAATACGCTCAGCCATGGAGAGGTGGTATGT GCTGTGACCATCAGCAACCCCACACGACACGTCTACACAGGCGGCAAGGGCTGTGTGAAGATATGGGACA TCAGCCAGCCGGGCAGCAAGAGTCCCATCTCCCAGCTGGACTGCCTGAACAGGGACAACTACATCCGCTC GTGCAAGCTTCTCCCCGATGGGCGCACGCTCATTGTGGGTGGTGAGGCCAGCACGCTCACCATCTGGGAC CTGGCCTCACCCACACCCCGCATCAAGGCTGAGCTGACGTCCTCGGCTCCAGCCTGTTATGCCCTGGCCA TCAGTCCTGATGCCAAAGTCTGTTTTTCCTGCTGCAGCGACGGGAACATTGCGGTTTGGGATCTGCACAA CCAGACCCTGGTCAGGCAGTTCCAGGGCCACACAGATGGGGCCAGCTGTATAGACATCTCTCATGATGGC ACTAAGCTGTGGACCGGGGGCCTGGACAACACCGTGCGCTCCTGGGACCTACGTGAAGGACGGCAGTTAC AGCAACACGATTTCACCTCCCAGATCTTCTCCCTGGGTTACTGCCCCACTGGGGAGTGGCTGGCCGTGGG CATGGAGAGCAGCAATGTGGAGGTCCTGCACCACACTAAGCCCGACAAATACCAGCTGCACCTGCACGAG AGCTGCGTGCTGTCCCTCAAGTTCGCCTATTGTGGCAAGTGGTTTGTGAGCACTGGGAAAGACAACCTTC TCAATGCCTGGAGGACGCCTTATGGAGCCAGCATCTTCCAGTCAAAAGAATCCTCATCTGTCTTGAGCTG TGACATTTCAGCGGATGACAAATATATTGTAACAGGCTCTGGTGACAAGAAGGCCACAGTTTACGAGGTC ATCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG223072 representing NM_009389 Red=Cloning site Green=Tags(s) MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYG LNIEMHKQTEIAKRLNTILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGQQQLQAQHLSHATHGP PVQLPPHPSGLQPPGIPPVTGSSSGLLALGALGSQAHLAVKDEKNHHELDHRERESSTNNSVSPSESLRA SEKHRGSADYSMEAKKRKAEEKDSLSRYDSDGDKSDDLVVDVSNEDPATPRVSPAHSPPENGLDKARGLK KDAPTSPASVASSSSTPSSKTKDLGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRSMPGKPPGMDPI GIMASALRTPITLTSSYPAPFAMMSHHEMNGSLTSPSAYAGLHNIPSQMSAAAAAAAAAYGRSPMVSFGA VGFDPHPPMRATGLPSSLASIPGGKPAYSFHVSADGQMQPVPFPHDALAGPGIPRHARQINTLSHGEVVC AVTISNPTRHVYTGGKGCVKIWDISQPGSKSPISQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLTIWD LASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISHDG TKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQLHLHE SCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKKATVYEV IY TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_009389
ORF Size	2316 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_009389.2, NP_033415.2
RefSeq Size	5172 bp
RefSeq ORF	2319 bp
Locus ID	21887
UniProt ID	Q08122
Gene Summary	Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity). May play an important role during spermatogenesis.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MC221488	Tle3 (untagged) - Mouse transducin-like enhancer of split 3, homolog of Drosophila E(spl) (Tle3), transcript variant 2, (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,980.00
MR223072	Tle3 (Myc-DDK-tagged) - Mouse transducin-like enhancer of split 3, homolog of Drosophila E(spl) (Tle3), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00
MR223072L3	Lenti ORF clone of Tle3 (Myc-DDK-tagged) - Mouse transducin-like enhancer of split 3, homolog of Drosophila E(spl) (Tle3), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,600.00
MR223072L4	Lenti ORF clone of Tle3 (mGFP-tagged) - Mouse transducin-like enhancer of split 3, homolog of Drosophila E(spl) (Tle3), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,600.00

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