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Ddr2 (NM_022563) Mouse Tagged ORF Clone
CAT＃: MG222870
- TrueORF®
Ddr2 (tGFP-tagged) - Mouse discoidin domain receptor family member 2 (Ddr2), (10ug)
ORF Plasmid: DDK
"NM_022563" in other vectors (4)
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CNY 9,410.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW495251; Ntrkr3; tyro10 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222870 representing NM_022563
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCCCGATTCCCAGAATGCCCCTGGTGCTGCTCCTGCTCTTGCTCATCCTGGGTTCTGCAAAAGCTC AGGTTAATCCAGCCATATGCCGCTATCCTCTGGGCATGTCAGGAGGCCACATTCCAGATGAGGACATCAC AGCCTCAAGTCAGTGGTCAGAATCCACAGCTGCCAAATATGGGAGGCTGGACTCTGAAGAAGGAGATGGA GCCTGGTGTCCTGAGATTCCAGTGCAACCCGATGACCTGAAGGAATTTTTGCAGATTGACTTGCGAACCC TACACTTTATCACTCTTGTGGGGACCCAGGGGCGCCATGCAGGGGGTCATGGCATTGAATTTGCACCCAT GTACAAGATCAACTACAGTCGGGATGGCAGTCGCTGGATCTCCTGGCGTAACCGGCATGGGAAGCAGGTG CTTGATGGAAACAGTAACCCTTATGATGTCTTCCTGAAGGACTTGGAGCCACCCATCGTCGCCAGATTTG TTCGCCTTATCCCAGTCACTGACCACTCCATGAACGTGTGCATGAGGGTTGAGCTTTATGGTTGTGTCTG GCTAGATGGCTTGGTATCCTACAATGCTCCAGCTGGACAGCAGTTTGTACTCCCTGGAGGCTCCATCATT TATCTGAATGATTCTGTCTATGATGGAGCTGTTGGGTACAGCATGACTGAAGGGCTAGGCCAGTTGACTG ATGGAGTATCCGGCCTGGATGATTTTACCCAGACCCATGAATACCACGTGTGGCCTGGCTATGACTACGT GGGATGGCGGAACGAAAGTGCTACCAACGGTTTCATTGAGATCATGTTTGAATTTGACCGAATCAGGAAT TTTACTACCATGAAGGTCCACTGCAACAATATGTTTGCTAAAGGTGTGAAGATTTTTAAGGAGGTCCAGT GCTACTTTCGCTCGGAAGCCAGCGAGTGGGAACCCACTGCTGTCTACTTTCCCCTGGTCCTGGACGATGT GAACCCCAGTGCCCGGTTTGTCACGGTGCCCCTCCACCACCGAATGGCCAGTGCCATCAAGTGCCAATAC CATTTTGCCGACACGTGGATGATGTTCAGCGAGATCACTTTCCAATCAGATGCTGCAATGTATAACAACT CTGGAGCCCTTCCCACCTCTCCTATGGCACCCACCACCTATGATCCCATGCTTAAAGTTGATGATAGCAA CACTCGGATCCTGATTGGTTGCTTGGTGGCCATCATCTTCATCCTGCTGGCTATCATCGTCATCATCCTG TGGAGGCAGTTCTGGCAGAAGATGCTAGAAAAGGCTTCACGGAGGATGCTGGATGATGAAATGACAGTCA GCCTTTCCCTGCCCAGCGAGTCCAGCATGTTCAATAACAACCGCTCCTCATCACCAAGTGAACAGGAGTC CAACTCTACTTATGATCGAATCTTCCCCCTTCGCCCTGACTACCAGGAGCCATCCAGACTGATCCGAAAG CTTCCAGAGTTTGCTCCAGGAGAGGAGGAGTCAGGGTGCAGTGGTGTTGTGAAGCCGGCCCAGCCCAATG GACCTGAGGGCGTGCCCCACTATGCAGAAGCCGACATAGTGAATCTCCAGGGAGTGACAGGTGGCAACAC CTACTGTGTGCCTGCTGTAACCATGGATCTGCTATCGGGGAAAGATGTGGCTGTGGAAGAGTTCCCCAGG AAACTGTTGGCCTTCAAGGAGAAGCTGGGAGAAGGCCAGTTTGGGGAGGTTCATCTCTGTGAAGTGGAGG GAATGGAAAAATTCAAAGACAAAGATTTTGCACTAGATGTCAGTGCCAACCAGCCTGTCCTGGTGGCCGT GAAAATGCTCCGAGCAGATGCCAACAAGAATGCCAGGAATGATTTTCTTAAGGAGATCAAGATCATGTCT CGGCTCAAGGACCCAAACATCATCCGTCTCTTAGCTGTGTGCATCACTGAGGACCCACTCTGCATGATCA CGGAATACATGGAAAATGGAGATCTTAATCAGTTTCTTTCTCGCCACGAGCCTCTGAGTTCCTGTTCTAG TGATGCCACAGTCAGTTATGCCAACCTGAAGTTTATGGCAACCCAGATTGCCTCTGGTATGAAGTACCTT TCGTCTCTCAACTTTGTCCACCGAGATCTGGCCACACGAAACTGCTTAGTGGGCAAGAATTACACCATCA AGATAGCTGATTTTGGCATGAGCAGAAACCTGTACAGTGGTGATTACTACCGGATCCAGGGCCGGGCGGT GCTCCCCATTCGCTGGATGTCCTGGGAAAGCATCTTGCTGGGCAAATTCACCACGGCAAGTGATGTGTGG GCCTTTGGGGTGACTCTGTGGGAGACTTTCACCTTTTGCCAGGAGCAGCCCTATTCCCAGCTGTCGGATG AGCAGGTTATCGAGAACACTGGAGAGTTTTTCCGAGACCAAGGGAGGCAGATCTATCTCCCTCAACCAGC CCTTTGCCCCGACTCTGTGTATAAGCTGATGCTCAGCTGCTGGAGAAGAGAAACCAAGCACCGGCCATCC TTCCAGGAAATACACCTCCTGCTTCTTCAGCAAGGAGCCGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222870 representing NM_022563
Red=Cloning site Green=Tags(s) MIPIPRMPLVLLLLLLILGSAKAQVNPAICRYPLGMSGGHIPDEDITASSQWSESTAAKYGRLDSEEGDG AWCPEIPVQPDDLKEFLQIDLRTLHFITLVGTQGRHAGGHGIEFAPMYKINYSRDGSRWISWRNRHGKQV LDGNSNPYDVFLKDLEPPIVARFVRLIPVTDHSMNVCMRVELYGCVWLDGLVSYNAPAGQQFVLPGGSII YLNDSVYDGAVGYSMTEGLGQLTDGVSGLDDFTQTHEYHVWPGYDYVGWRNESATNGFIEIMFEFDRIRN FTTMKVHCNNMFAKGVKIFKEVQCYFRSEASEWEPTAVYFPLVLDDVNPSARFVTVPLHHRMASAIKCQY HFADTWMMFSEITFQSDAAMYNNSGALPTSPMAPTTYDPMLKVDDSNTRILIGCLVAIIFILLAIIVIIL WRQFWQKMLEKASRRMLDDEMTVSLSLPSESSMFNNNRSSSPSEQESNSTYDRIFPLRPDYQEPSRLIRK LPEFAPGEEESGCSGVVKPAQPNGPEGVPHYAEADIVNLQGVTGGNTYCVPAVTMDLLSGKDVAVEEFPR KLLAFKEKLGEGQFGEVHLCEVEGMEKFKDKDFALDVSANQPVLVAVKMLRADANKNARNDFLKEIKIMS RLKDPNIIRLLAVCITEDPLCMITEYMENGDLNQFLSRHEPLSSCSSDATVSYANLKFMATQIASGMKYL SSLNFVHRDLATRNCLVGKNYTIKIADFGMSRNLYSGDYYRIQGRAVLPIRWMSWESILLGKFTTASDVW AFGVTLWETFTFCQEQPYSQLSDEQVIENTGEFFRDQGRQIYLPQPALCPDSVYKLMLSCWRRETKHRPS FQEIHLLLLQQGAE TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022563 |
| ORF Size | 2562 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022563.2, NP_072075.2 |
| RefSeq Size | 8306 bp |
| RefSeq ORF | 2565 bp |
| Locus ID | 18214 |
| UniProt ID | Q62371 |
| Gene Summary | Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell differentiation, remodeling of the extracellular matrix, cell migration and cell proliferation. Required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222147 | Ddr2 (untagged) - Mouse discoidin domain receptor family, member 2 (Ddr2), (10ug) |
CNY 9,392.00 |
|
| MR222870 | Ddr2 (Myc-DDK-tagged) - Mouse discoidin domain receptor family, member 2 (Ddr2) |
CNY 8,550.00 |
|
| MR222870L3 | Lenti ORF clone of Ddr2 (Myc-DDK-tagged) - Mouse discoidin domain receptor family, member 2 (Ddr2) |
CNY 10,450.00 |
|
| MR222870L4 | Lenti ORF clone of Ddr2 (mGFP-tagged) - Mouse discoidin domain receptor family, member 2 (Ddr2) |
CNY 10,450.00 |
