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Tead1 (NM_001166584) Mouse Tagged ORF Clone
CAT＃: MG222095
- TrueORF®
Tead1 (tGFP-tagged) - Mouse TEA domain family member 1 (Tead1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001166584" in other vectors (4)
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CNY 3,710.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2610024B07Rik; B230114H05Rik; Gtrgeo5; mTEF-1; Tcf13; TEAD-1; TEF-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222095 representing NM_001166584
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAGGATGAGCGACTCGGCAGATAAGCCGATTGACAACGACGCGGAGGGCGTCTGGAGTCCTGATA TTGAGCAGAGTTTCCAGGAGGCCCTGGCTATCTATCCGCCGTGTGGGAGGAGAAAAATCATCTTATCAGA CGAAGGCAAAATGTATGGTAGAAATGAATTGATAGCCAGATACATCAAACTCAGGACGGGAAAGACAAGG ACCAGGAAGCAGGTGTCTAGTCATATACAGGTCTTAGCAAGAAAGAAAGTTCGAGAAATTCAAGCCGCCA TTAAGGTGTCTAGTCACATTCAGGTTCTTGCCAGAAGGAAATCTCGTGATTTTCATTCCAAGCTGAAGGT AACAAGCATGGATCAGACTGCCAAGGACAAGGCCCTGCAGCACATGGCTGCCATGTCATCAGCCCAGATC GTCTCGGCTACTGCCATCCACAACAAGCTGGGGCTGCCTGGGATTCCACGCCCCACCTTCCCGGGGGGTC CGGGGTTCTGGCCTGGGATGATACAGACAGGACAGCCAGGATCCTCACAAGACGTCAAGCCCTTTGTGCA GCAGGCCTACCCCATCCAGCCAGCAGTCACAGCCCCCATTCCAGGGTTTGAGCCTACGTCAGCCCCAGCC CCCTCAGTTCCTGCCTGGCAGGGCCGATCCATTGGCACAACCAAGCTTCGCCTGGTGGAATTCTCCGCTT TCCTTGAACAGCAGAGAGACCCAGACTCGTACAACAAACACCTCTTCGTGCACATCGGGCATGCCAACCA TTCTTACAGTGACCCGTTGCTCGAATCTGTGGACATTCGTCAGATATATGACAAATTTCCTGAAAAGAAA GGTGGCTTGAAGGAGCTGTTTGGAAAGGGCCCTCAAAACGCCTTCTTCCTCGTCAAATTCTGGGCGGACT TAAACTGCAATATCCAAGACGACGCCGGGGCCTTTTATGGTGTGAGCAGTCAGTATGAGAGTTCTGAGAA CATGACAGTTACCTGTTCCACCAAAGTGTGCTCCTTTGGGAAACAAGTAGTAGAAAAAGTAGAGACGGAG TATGCGAGGTTCGAGAATGGTCGATTCGTGTACCGAATAAACCGCTCGCCAATGTGTGAATATATGATCA ACTTCATCCACAAGCTCAAACACCTACCAGAGAAATATATGATGAACAGTGTTTTGGAAAACTTCACCAT ATTATTGGTGGTAACAAACAGGGATACACAAGAAACTCTGCTCTGCATGGCCTGTGTATTTGAAGTCTCG AATAGCGAACACGGAGCACAGCACCATATCTACAGGCTTGTGAAGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222095 representing NM_001166584
Red=Cloning site Green=Tags(s) MERMSDSADKPIDNDAEGVWSPDIEQSFQEALAIYPPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTR TRKQVSSHIQVLARKKVREIQAAIKVSSHIQVLARRKSRDFHSKLKVTSMDQTAKDKALQHMAAMSSAQI VSATAIHNKLGLPGIPRPTFPGGPGFWPGMIQTGQPGSSQDVKPFVQQAYPIQPAVTAPIPGFEPTSAPA PSVPAWQGRSIGTTKLRLVEFSAFLEQQRDPDSYNKHLFVHIGHANHSYSDPLLESVDIRQIYDKFPEKK GGLKELFGKGPQNAFFLVKFWADLNCNIQDDAGAFYGVSSQYESSENMTVTCSTKVCSFGKQVVEKVETE YARFENGRFVYRINRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILLVVTNRDTQETLLCMACVFEVS NSEHGAQHHIYRLVKD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001166584 |
| ORF Size | 1308 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001166584.1, NP_001160056.1 |
| RefSeq Size | 9962 bp |
| RefSeq ORF | 1311 bp |
| Locus ID | 21676 |
| Gene Summary | Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
MicroRNA-497-5p negatively regulates the proliferation and cisplatin resistance of non-small cell lung cancer cells by targeting YAP1 and TEAD1
,null,
Translational Cancer Research
,PubMed ID 35116999
[Tead1]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC215724 | Tead1 (untagged) - Mouse TEA domain family member 1 (Tead1), transcript variant 1, (10ug) |
CNY 5,610.00 |
|
| MR222095 | Tead1 (Myc-DDK-tagged) - Mouse TEA domain family member 1 (Tead1), transcript variant 1 |
CNY 3,330.00 |
|
| MR222095L3 | Lenti ORF clone of Tead1 (Myc-DDK-tagged) - Mouse TEA domain family member 1 (Tead1), transcript variant 1 |
CNY 5,230.00 |
|
| MR222095L4 | Lenti ORF clone of Tead1 (mGFP-tagged) - Mouse TEA domain family member 1 (Tead1), transcript variant 1 |
CNY 5,230.00 |
