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Nsmf (NM_001177655) Mouse Tagged ORF Clone
CAT＃: MG221708
- TrueORF®
Nsmf (tGFP-tagged) - Mouse nasal embryonic LHRH factor (Nelf) transcript variant 5, (10ug)
ORF Plasmid: DDK
"NM_001177655" in other vectors (4)
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CNY 5,510.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Jacob; Nelf |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221708 representing NM_001177655
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGCCGCCGCCTCCAGGAGGAGGGCGCTGAGGAGCGAGGCCATGTCCTCGGTAGCGGCCAAAGTAA GAGCAGCCCGAGCGTTTGGAGAGTACCTGTCCCAGAGTCACCCTGAGAACCGCAACGGTGCAGACCACCT GCTGGCTGACGCCTACTCTGGCCACGACGGGTCCCCAGAGATGCAACCTGCACCCCAGAACAAGCGCCGC CTCTCCCTCGTCTCCAATGGCCGATATGAGGGCAGCATCTCAGATGAGGCAGTCAGCGGGAAGCCGGCTA TAGAGGGCCCCCAGCCCCACGTGTACACCATCTCTAGAGAGCCCGCCCTGCTACCTGGCTCTGAAGCTGA AGCCATTGAGCTAGCAGTGGTAAAAGGGAGGAGACAGCGGGAACGGCACCCTCACCACCACAGCCAGCCC CTGCGTGCCAGCCCAGGCAGCAGCCGGGAGGACATCAGCAGGCCCTGCCAAAGCTGGGCAGGAAGCCGCC AGGGCTCCAAAGAGTGCCCAGGATGTGCCCAGCTGGTCCCTGGTCCCTCTTCTCGGGCCTTTGGGCTGGA GCAGCCACCTCTACCTGAGGCTCCTGGCCGCCACAAGAAGCTGGAAAGGATGTATAGCGTTGATGGAGTG TCTGATGATGTCCCCATCCGTACCTGGTTCCCCAAGGAAAACCTTTTCAGCTTCCAGACGGCAACCACAA CTATGCAAGCGGTATTCAGGGGCTACGCGGAGAGGAAGCGTCGGAAACGGGAGAATGATTCCGCGTCTGT AATCCAGAGGAACTTCCGCAAACACCTGCGCATGGTTGGCAGTCGGCGGGTGAAGGCCCAGAGCAGTGAC CTACAGAGCTCACACTGCACCCTGGATGAGGCTTGTGAGGACCTGGACTGGGACACAGAGAAAGGTCTGG AGGCCATGGCCTGCAACACCGAGGGCTTCTTGCCACCCAAGGTCATGCTCATCTCCTCCAAGGTGCCAAA GGCCGAGTATATCCCTACTATCATCCGCAGAGATGACCCGTCCATCATTCCCATCCTCTACGACCACGAG CATGCAACCTTCGAGGACATCCTGGAGGAAATAGAGAAGAAATTGAACATCTATCACAAGGGGGCCAAGA TCTGGAAGATGCTGATTTTCTGCCAGGGCGGCCCTGGACACCTTTATTTGCTCAAGAACAAGGTGGCCAC CTTTGCCAAAGTGGAGAAGGAAGAGGACATGATCCACTTCTGGAAGAGGCTGAGCCGCCTGATGAGCAAG GTGAACCCCGAGCCGAATGTCATCCACATCATGGGCTGCTACATTCTGGGGAACCCTAACGGGGAGAAGC TATTCCAGAACCTCAGGACCCTCATGACCCCTTACAAGGTTACCTTTGAGTCGCCTCTCGAGCTGTCTGC CCAAGGGAAGCAGATGATTGAGACCTACTTTGACTTCCGGCTGTACCGCCTGTGGAAGAGCCGCCAGCAC TCAAAGCTGCTGGACTTTGATGACGTCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221708 representing NM_001177655
Red=Cloning site Green=Tags(s) MGAAASRRRALRSEAMSSVAAKVRAARAFGEYLSQSHPENRNGADHLLADAYSGHDGSPEMQPAPQNKRR LSLVSNGRYEGSISDEAVSGKPAIEGPQPHVYTISREPALLPGSEAEAIELAVVKGRRQRERHPHHHSQP LRASPGSSREDISRPCQSWAGSRQGSKECPGCAQLVPGPSSRAFGLEQPPLPEAPGRHKKLERMYSVDGV SDDVPIRTWFPKENLFSFQTATTTMQAVFRGYAERKRRKRENDSASVIQRNFRKHLRMVGSRRVKAQSSD LQSSHCTLDEACEDLDWDTEKGLEAMACNTEGFLPPKVMLISSKVPKAEYIPTIIRRDDPSIIPILYDHE HATFEDILEEIEKKLNIYHKGAKIWKMLIFCQGGPGHLYLLKNKVATFAKVEKEEDMIHFWKRLSRLMSK VNPEPNVIHIMGCYILGNPNGEKLFQNLRTLMTPYKVTFESPLELSAQGKQMIETYFDFRLYRLWKSRQH SKLLDFDDVL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001177655 |
| ORF Size | 1500 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001177655.1, NP_001171126.1 |
| RefSeq Size | 2853 bp |
| RefSeq ORF | 1503 bp |
| Locus ID | 56876 |
| UniProt ID | Q99NF2 |
| Gene Summary | Couples NMDA-sensitive glutamate receptor signaling to the nucleus and triggers long-lasting changes in the cytoarchitecture of dendrites and spine synapse processes. Part of the cAMP response element-binding protein (CREB) shut-off signaling pathway. Stimulates outgrowth of olfactory axons and migration of gonadotropin-releasing hormone (GnRH) and luteinizing-hormone-releasing hormone (LHRH) neuronal cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217001 | Nsmf (untagged) - Mouse nasal embryonic LHRH factor (Nelf), transcript variant 5, (10ug) |
CNY 6,460.00 |
|
| MR221708 | Nsmf (Myc-DDK-tagged) - Mouse nasal embryonic LHRH factor (Nelf), transcript variant 5 |
CNY 5,040.00 |
|
| MR221708L3 | Lenti ORF clone of Nsmf (Myc-DDK-tagged) - Mouse nasal embryonic LHRH factor (Nelf), transcript variant 5 |
CNY 6,940.00 |
|
| MR221708L4 | Lenti ORF clone of Nsmf (mGFP-tagged) - Mouse nasal embryonic LHRH factor (Nelf), transcript variant 5 |
CNY 6,940.00 |
