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Irs4 (NM_010572) Mouse Tagged ORF Clone
CAT＃: MG221096
- TrueORF®
Irs4 (tGFP-tagged) - Mouse insulin receptor substrate 4 (Irs4), (10ug)
ORF Plasmid: DDK
"NM_010572" in other vectors (2)
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CNY 11,690.00
货期*
7周
规格
| Cited in 1 publication. |
Product images
经常一起买 (5)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | IRS-4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221096 representing NM_010572
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGTTGCTCCTTCTCTGGCCACCAAGCGCTTAGGAGACTGAGAGCCTCAGCAGCAGCAGCAGCCT CCGCAGCTCTAGCAGCAGTGGCGACCACCCCGCTCCTTTCCTCGGGAACCCGGACCGCACTCATTGGGAC CGGGTCGTCCTGTCCGGGAGCCATGTGGCTCTCCACAGCCACTGGCTCCCGGTCAGACTCGGAATCCGAA GAGGAGGACCTCCCCGTCGGGGATGAAGTCTGCAAACGCGGCTACCTGAGGAAGCAGAAGCATGGGCATA GGCGTTACTTCGTGCTCAAACTCGAGACCGCAGACGCTCCAGCTCGACTCGAATACTACCGAAATGCCAG GAAGTTCCGGCACAGTGTCCGCGCCGCGGCGGCTGCAGCAGAGGCGGCCGCCTCCGGTGCTGCGGTCCCC GCGCTCATCCCACCACGGCGCGTGATCATCCTGTACCAATGCTTCTCCGTGAGCCAGCGCGCCGATGCCA GGTACCGACACCTCATTGCCCTTTTTACCCAGGACGAATACTTCGCGATGGTGGCCGAGAACGAGTCGGA ACAAGAAAGCTGGTACTTGCTCCTCAGCCGCCTCATCCTCGAGAGCAAGCGCCGCCGCTGCGGCACGCTA GGCGCACTGCCGGACGGAGAGCCGGCAGCCCTGGCGGCCGCAGCGGCGGCGGAGCCACCCTTCTACAAAG ATGTGTGGCAGGTAGTAGTGAAACCCAGGGGGCTGGGGCACAGAAAAGAGCTGAGCGGTGTGTTCCGGCT GTGTCTTACCGACGAAGAGGTAGTGTTTGTAAGGCTCAATACCGAAGTGGCCAGTGTGGTCGTCCAGCTG CTGAGCATTCGTCGCTGTGGGCACTCGGAGCAGTACTTCTTCTTGGAAGTCGGCAGATCCACTGTCATAG GTCCGGGGGAGCTGTGGATGCAAGTGGATGACTCCGTGGTGGCCCAAAATATGCATGAGCTGTTTCTGGA GAAGATGAGAGCCTTGTGCGCAGATGAATACAGAGCCCGATGCCGCAGCTACAGCATCAGTATTGGCGCC CACCTGTTAACCCTGCTGTCCACTAGGAGGCACCTGGGCTTGCTCCCGCTGGAGCCTGGTGGCTGGCTCA GAAGATACGGCTTGGAGCAGTTTTGCCGCCTCAGAGCTATCCGTGAGAGGGAAGAGATGCTATTCACCAG GCGCTTCATCTCACCCAGAGAGCCTCCGCCTCCCTTTAGGCGAGGAAGAGGGCACCTGCCCAGAGCCCGC AGGTCCAGGAGAGCCGCGTCAGTACCACCCAGCCTTTTCCGACGCTCAGCACCTAGCCCAGGAAGGATCC CACAGCCTGAAGATGTCCCCAACGACAGAGCCCGTGAAGCCTCCGGTTCCAGCTCTGGCAACACTGAAGA AAAAGACAAGGAGGGTGAGGAAGGAAACCGAGGTGATTGCATACCTATGAACAACTGGGGCTCAGGAAAT GGCCGGGGCTCAGGAGGTGGACGGGGCTCAAGTGGCCAAGGTTCCAGTAGCCAGGGCTCAGGTGGGAGAC AGGGATCTGGAGGTGGCCAGGGCTCAGGAGGCCAGGGTGCCGGAGGAAACCAGTGTTCAGGAAATGGCCA GGGCACAGCAGGTGGCCATGGCTCAGGTGGAGGTGGCCATGGCTCAGGCGGTGGCCAGAGACCTGGAGAT GGTCATGGCTCAGGTGGTGGCAAGAACTCAGGAAGTGGGAAAAATTCTGACGATGGTGACCGTGGAAAAT CTGTGAAGAAAAGATCCTACTTTGGTAAATTCACTCAAAGCAAGCAGCAGCAAACACTGCCTCCACCCCC ACCGCCACCGCCAGCAGCTGGAGCAACTGGTGGCAAAGGGAAGTCTGGAGGAAGATTCCGACTTTATTTT TGCGCAGACAGAGGCACAAAAGAACGCAAAGAAGCCAAAGAGGTAAGAGATATGGAGACCCCAGGAGGTG CTACCCGGGGGCCTTACAGAGCCAGAGCTTTCGATGAAGATGAGGATGACCCGTATGTGCCAATGAGACC AGGGGTGGCTGCCCCTCTTGCGTGTTCCAGTGATTATATGCCAATGGCTCCTCAAAATTCCTCTGCTTCA ACAAAACGCCACTCTAGGTCACCCTTTGAAGACTCCAGAGGGTACATGATGATGTTTCCCAGAGTGAGCC CCCCACCACCTGTGCCAAGTGCTCCGAAAGCACCGGACACCAATAAAGGGGATGACTCAAAGGACAATGA CAGTGACAGTGATTACATGTTTATGGCTCCCGGAGCAGGTGCGATTCCTAAAAATCCTCCCAATGCGCAG GGAGGCTCTTCCTCCAAAAGCTGGAGCTCGTATTTCTCTCTGCCAAGTCCTTTTCAGAGTTCACCTTTGG GACAGAGTGACCACAGTGAGTATGTTCCTATGTTACCTGGAAAATTCCTGGGGAGCGGTCTACACAAAGA AGCCTCCTTTAGCCAGGGCACCAAAAATGTGTCTTCAAAGCCTTCAACTGAGGCGTCATTCTCAAAACCC GAAGATAAAGGGTCATCTGCAAAGCCTTCAGATGATGTGCCCCCAATGAACAAGGCTAAGGAACCTAACC ACCTTTCTTTTATTGCAAAAGGAACTCAAGTAAAGCCCAAACCGCTAAACCCCACACAGGAGCGGAGAGA AGCTGCTGGCTCCCGTGACTATATCAACATTGACTTTATTAAGAGAGAGCGCCTTGTGCTACCTTCCTCT GCTCAAGGACTGCCAGACATGCGGGGCGTAGTTACTGACCCCGCACCCACAGCTTTTTCTGGCTACCTGA ATGTTGAATTCGGGGTGCCCTTCCCAAATCCAACAATCCGCCTCTCAGATCTTTTAAGAGTTCTACCAGG TGCCAACTCCATCCCTCTGGCCGGTACTAGGTGGCCCTTCCCAGGCAGTGCTATAGGTAGCATTGTGGAA GCGGGAGAATACATTGAAGTTATTTTCAACCCGGCTATGACACCAGCCATGTCTTTTGCTGACAGTGCCA TTTGCTATGATGCTCAAACAGGTCAAATCTATGTCGTCGACCCATTTTCTGAGTGCTGTATGGACGTTTC TCTCTCTCCTGGTCGATGCTCTGAACCGCCACCTGTAGCTAGGCTGCGGCGGGAGGAAGCGCAGGAGAGA AGACGCCCACAAAGCCGCTCCCAAAGTTTATTTGCCAGCACTCGAGCCGCGGTCTCGGCTTTCCCGACTG ACAGCTTGGACAGAGACTTTCCCGCCGCTTCTGCGGTGATTGCTGCTCCAGCTGAGGCGCCTCTCTTGGC AGTGAGCCGGGCTTTAGCCGTGGTCTCTGCGCTCGCAGCGGCCCCCAGCATAGGCGACGTCTTTGCAGGC TTCCGCGCTGCGGCAGGAGTTGACTCCGCCTCCGCTCGCGGGTTCCAACCTGTTGCGGGTGCCCAGGCTG TAAGAGAATTCCAGGACCTTGCCGCAGGCTGGAATCCTGGAGCCCTTAACCACGGGGCCCGAGGTGAGGA CCTGGCCGCCGGCGCAGCGGCTCCCCCGCCGCCACCTCGCCAGATTTGGGTGCTGAGACCCCAAGAGAGA GCGGATTCTGAGGACGACGACGATGATGACGACGACATTTACGTGAGAATGGACTTTGCCAGACGAGACT ACAGGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221096 representing NM_010572
Red=Cloning site Green=Tags(s) MASCSFSGHQALRRLRASAAAAASAALAAVATTPLLSSGTRTALIGTGSSCPGAMWLSTATGSRSDSESE EEDLPVGDEVCKRGYLRKQKHGHRRYFVLKLETADAPARLEYYRNARKFRHSVRAAAAAAEAAASGAAVP ALIPPRRVIILYQCFSVSQRADARYRHLIALFTQDEYFAMVAENESEQESWYLLLSRLILESKRRRCGTL GALPDGEPAALAAAAAAEPPFYKDVWQVVVKPRGLGHRKELSGVFRLCLTDEEVVFVRLNTEVASVVVQL LSIRRCGHSEQYFFLEVGRSTVIGPGELWMQVDDSVVAQNMHELFLEKMRALCADEYRARCRSYSISIGA HLLTLLSTRRHLGLLPLEPGGWLRRYGLEQFCRLRAIREREEMLFTRRFISPREPPPPFRRGRGHLPRAR RSRRAASVPPSLFRRSAPSPGRIPQPEDVPNDRAREASGSSSGNTEEKDKEGEEGNRGDCIPMNNWGSGN GRGSGGGRGSSGQGSSSQGSGGRQGSGGGQGSGGQGAGGNQCSGNGQGTAGGHGSGGGGHGSGGGQRPGD GHGSGGGKNSGSGKNSDDGDRGKSVKKRSYFGKFTQSKQQQTLPPPPPPPPAAGATGGKGKSGGRFRLYF CADRGTKERKEAKEVRDMETPGGATRGPYRARAFDEDEDDPYVPMRPGVAAPLACSSDYMPMAPQNSSAS TKRHSRSPFEDSRGYMMMFPRVSPPPPVPSAPKAPDTNKGDDSKDNDSDSDYMFMAPGAGAIPKNPPNAQ GGSSSKSWSSYFSLPSPFQSSPLGQSDHSEYVPMLPGKFLGSGLHKEASFSQGTKNVSSKPSTEASFSKP EDKGSSAKPSDDVPPMNKAKEPNHLSFIAKGTQVKPKPLNPTQERREAAGSRDYINIDFIKRERLVLPSS AQGLPDMRGVVTDPAPTAFSGYLNVEFGVPFPNPTIRLSDLLRVLPGANSIPLAGTRWPFPGSAIGSIVE AGEYIEVIFNPAMTPAMSFADSAICYDAQTGQIYVVDPFSECCMDVSLSPGRCSEPPPVARLRREEAQER RRPQSRSQSLFASTRAAVSAFPTDSLDRDFPAASAVIAAPAEAPLLAVSRALAVVSALAAAPSIGDVFAG FRAAAGVDSASARGFQPVAGAQAVREFQDLAAGWNPGALNHGARGEDLAAGAAAPPPPPRQIWVLRPQER ADSEDDDDDDDDIYVRMDFARRDYRK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010572 |
| ORF Size | 3648 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010572.2, NP_034702.2 |
| RefSeq Size | 6269 bp |
| RefSeq ORF | 3651 bp |
| Locus ID | 16370 |
| UniProt ID | Q9Z0Y7 |
| Gene Summary | Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis. Interaction with GRB2 enhances insulin-stimulated mitogen-activated protein kinase activity. May be involved in nonreceptor tyrosine kinase signaling in myoblasts. Plays a pivotal role in the proliferation/differentiation of hepatoblastoma cell through EPHB2 activation upon IGF1 stimulation. May play a role in the signal transduction in response to insulin and to a lesser extent in response to IL4 and GH on mitogenesis. Plays a role in growth, reproduction and glucose homeostasis. May act as negative regulators of the IGF1 signaling pathway by suppressing the function of IRS1 and IRS2.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A BRET assay for monitoring insulin receptor interactions and ligand pharmacology.
,null,
Journal of receptor and signal transduction research
,PubMed ID 22272819
[Irs4]
|
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