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Lgr4 (NM_172671) Mouse Tagged ORF Clone
CAT＃: MG219497
- TrueORF®
Lgr4 (tGFP-tagged) - Mouse leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4), (10ug)
ORF Plasmid: DDK
"NM_172671" in other vectors (4)
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CNY 9,220.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9130225G07; A330106J01Rik; A930009A08Rik; Gpr48 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG219497 representing NM_172671
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGGCCCGCTGCGGCTGCTCTGCTTCTTCGCCCTGGGGCTGCTCGGCTCGGCCGGGCCCAGCGGCG CGGCGCCACCTCTCTGCGCTGCGCCCTGCAGCTGCGACGGCGACCGTCGGGTGGACTGCTCCGGGAAGGG GTTGACGGCGGTACCGGAGGGGCTCAGCGCCTTCACCCAAGCACTGGATATCAGTATGAACAATATCACT CAGTTACCAGAAGATGCATTTAAGAATTTTCCTTTTCTAGAGGAGCTACAACTGGCTGGTAACGACCTTT CTTTTATCCACCCAAAAGCCTTGTCTGGGTTGAAAGAACTCAAAGTCCTAACCCTCCAGAACAATCAGTT GAAAACAGTACCCAGTGAAGCCATTCGTGGACTGAGTGCTTTGCAGTCTCTACGCTTAGATGCCAACCAT ATTACCTCAGTCCCGGAGGACAGTTTTGAAGGGCTCGTTCAGTTGCGGCATCTGTGGCTGGATGACAACA TCTTGACGGAAGTGCCTGTGCGTCCGCTCAGCAACCTGCCAACCCTGCAGGCGCTGACCTTGGCTCTCAA CAACATCTCAAGCATCCCCGACTTCGCATTCACCAACCTTTCAAGCTTGGTAGTGCTGCATCTTCATAAC AATAAAATTAAAAGCCTCAGTCAACACTGTTTTGATGGACTAGATAACCTGGAAACCCTGGACTTGAATT ATAATAACTTGGATGAATTTCCTCAGGCTATTAAAGCCCTTCCCAGCCTTAAAGAGCTGGGATTTCACAG TAATTCTATTTCTGTTATCCCGGATGGAGCATTTGCTGGTAATCCACTGCTAAGAACTATCCATTTGTAT GATAATCCTCTGTCTTTTGTGGGGAACTCAGCATTTCACAACCTGTCTGATCTGCATTCCTTAGTCATTC GTGGTGCAAGCCTGGTGCAGTGGTTCCCCAATCTGGCCGGAACTGTCCATCTGGAGAGTCTAACCTTGAC AGGGACAAAAATAAGCAGCATACCTGATGATCTGTGCCAAAACCAAAAGATGCTGCGGACTCTGGACTTA TCTTATAACGATATAAGAGACCTTCCAAGTTTTAATGGTTGTCGTGCATTGGAAGAAATTTCATTGCAGC GTAATCAAATCTCCCTGATAAAGGAAACTACTTTTCAAGGCCTAACATCCCTAAGGATTCTAGATCTGAG TAGAAACCTGATTCGTGAAATTCACAGTGGAGCTTTTGCGAAGCTTGGGACAATTACTAACCTGGATGTG AGTTTCAATGAATTAACCTCATTTCCTACGGAAGGCCTGAATGGGCTCAATCAACTTAAACTTGTGGGTA ACTTCCAGCTGAAAGATGCCTTGGCAGCCAGAGACTTTGCCAATCTCAGGTCTCTATCAGTACCATATGC TTATCAGTGTTGTGCATTTTGGGGGTGTGACTCTTATGCAAATTTAAACACAGAAGATAACAGCCCCCAA GACCACAGTGTGACAAAAGAGAAAGGTGCTACAGATGCAGCAAATGCCACCAGCACTGCTGAAAGTGAAG AACATAGCCAAATAATCATCCATTGTACACCTTCAACAGGTGCTTTTAAGCCCTGTGAATATTTACTGGG AAGCTGGATGATTCGCCTTACAGTGTGGTTCATTTTCCTGGTCGCCTTGCTTTTCAACCTGCTTGTCATT TTAACAGTGTTTGCGTCTTGTTCATCACTGCCTGCCTCCAAGCTCTTCATAGGCTTGATTTCTGTGTCTA ACTTACTCATGGGCATCTATACTGGCATCCTTACTTTTCTTGATGCTGTGTCCTGGGGCCGATTTGCTGA ATTTGGCATTTGGTGGGAAACTGGCAGCGGCTGCAAGGTAGCTGGGTCTCTGGCAGTCTTCTCCTCAGAG AGCGCCGTATTCCTGTTAACCCTGGCAGCCGTGGAAAGAAGCGTTTTTGCAAAGGATGTAATGAAAAATG GGAAAAGCAGTCACCTCCGACAGTTCCAGGTGGCTGCCCTCGTAGCTTTGCTGGGTGCTGCAATAGCAGG CTGCTTCCCCCTTTTTCACGGAGGGCAATATTCTGCATCACCCTTGTGCTTGCCATTTCCTACAGGAGAG ACACCATCATTAGGATTCACTGTGACCCTAGTGCTATTAAACTCACTAGCATTTTTATTGATGGCCATTA TCTACACTAAACTCTACTGCAACTTAGAGAAAGAAGACCCGTCAGAAAACTCCCAGTCTAGCATGATTAA GCACGTTGCTTGGCTCATCTTCACAAACTGCATCTTCTTCTGCCCTGTTGCATTTTTCTCATTCGCACCA TTGATCACGGCAATCTCCATCAGCCCCGAGATAATGAAGTCTGTTACGCTGATATTCTTCCCGCTGCCTG CTTGCCTGAATCCAGTCCTGTACGTTTTCTTCAACCCAAAGTTTAAAGACGACTGGAAGCTCCTGAAGCG GCGTGTCACCAGGAAACACGGATCAGTCTCAGTCTCCATCAGCAGCCAAGGCGGTTGTGGGGAACAGGAT TTCTACTACGACTGTGGCATGTATTCCCACTTGCAGGGTAACCTGACTGTCTGTGACTGCTGTGAGTCAT TTCTTCTGACAAAACCAGTATCGTGCAAACACTTAATAAAATCGCACAGTTGTCCTGTATTGACAGTGGC CTCTTGCCAGAGGCCAGAGGCCTACTGGTCTGATTGTGGCACACAGTCGGCCCATTCTGACTATGCAGAT GAAGAGGATTCCTTTGTCTCGGACAGCTCTGACCAGGTGCAGGCCTGTGGACGAGCCTGCTTCTACCAGA GTCGCGGATTCCCTCTGGTGCGCTATGCTTATAATCTACCGAGAGTCAGAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219497 representing NM_172671
Red=Cloning site Green=Tags(s) MPGPLRLLCFFALGLLGSAGPSGAAPPLCAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQALDISMNNIT QLPEDAFKNFPFLEELQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANH ITSVPEDSFEGLVQLRHLWLDDNILTEVPVRPLSNLPTLQALTLALNNISSIPDFAFTNLSSLVVLHLHN NKIKSLSQHCFDGLDNLETLDLNYNNLDEFPQAIKALPSLKELGFHSNSISVIPDGAFAGNPLLRTIHLY DNPLSFVGNSAFHNLSDLHSLVIRGASLVQWFPNLAGTVHLESLTLTGTKISSIPDDLCQNQKMLRTLDL SYNDIRDLPSFNGCRALEEISLQRNQISLIKETTFQGLTSLRILDLSRNLIREIHSGAFAKLGTITNLDV SFNELTSFPTEGLNGLNQLKLVGNFQLKDALAARDFANLRSLSVPYAYQCCAFWGCDSYANLNTEDNSPQ DHSVTKEKGATDAANATSTAESEEHSQIIIHCTPSTGAFKPCEYLLGSWMIRLTVWFIFLVALLFNLLVI LTVFASCSSLPASKLFIGLISVSNLLMGIYTGILTFLDAVSWGRFAEFGIWWETGSGCKVAGSLAVFSSE SAVFLLTLAAVERSVFAKDVMKNGKSSHLRQFQVAALVALLGAAIAGCFPLFHGGQYSASPLCLPFPTGE TPSLGFTVTLVLLNSLAFLLMAIIYTKLYCNLEKEDPSENSQSSMIKHVAWLIFTNCIFFCPVAFFSFAP LITAISISPEIMKSVTLIFFPLPACLNPVLYVFFNPKFKDDWKLLKRRVTRKHGSVSVSISSQGGCGEQD FYYDCGMYSHLQGNLTVCDCCESFLLTKPVSCKHLIKSHSCPVLTVASCQRPEAYWSDCGTQSAHSDYAD EEDSFVSDSSDQVQACGRACFYQSRGFPLVRYAYNLPRVRD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172671 |
| ORF Size | 2853 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172671.2, NP_766259.2 |
| RefSeq Size | 5066 bp |
| RefSeq ORF | 2856 bp |
| Locus ID | 107515 |
| UniProt ID | A2ARI4 |
| Gene Summary | Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye. May also act as a receptor for norrin (NDP), such results however require additional confirmation in vivo. Required during spermatogenesis to activate the Wnt signaling pathway in peritubular myoid cells. Required for the maintenance of intestinal stem cells and Paneth cell differentiation in postnatal intestinal crypts. Acts as a regulator of bone formation and remodeling. Involved in kidney development; required for maintaining the ureteric bud in an undifferentiated state. Involved in the development of the anterior segment of the eye. Required during erythropoiesis. Also acts as a negative regulator of innate immunity by inhibiting TLR2/TLR4 associated pattern-recognition and proinflammatory cytokine production. Plays an important role in regulating the circadian rhythms of plasma lipids, partially through regulating the rhythmic expression of MTTP (PubMed:24353284).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222799 | Lgr4 (untagged) - Mouse leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4), (10ug) |
CNY 6,976.00 |
|
| MR219497 | Lgr4 (Myc-DDK-tagged) - Mouse leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4) |
CNY 6,968.00 |
|
| MR219497L3 | Lenti ORF clone of Lgr4 (Myc-DDK-tagged) - Mouse leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4) |
CNY 10,260.00 |
|
| MR219497L4 | Lenti ORF clone of Lgr4 (mGFP-tagged) - Mouse leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4) |
CNY 10,260.00 |
