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Pym1 (NM_030100) Mouse Tagged ORF Clone
CAT＃: MG218272
- TrueORF®
Wibg (tGFP-tagged) - Mouse within bgcn homolog (Drosophila) (Wibg) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_030100" in other vectors (4)
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CNY 2,190.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | A030010B05Rik; Pym; Wibg |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218272 representing NM_030100
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAACCGCCAGCACCCCCGAGGCCACGGGGACAGGCAAGTACATTGCCTCAACACAGAGACCCGACG GGACGTGGCGAAAGCAGCGGAGGGTCAAAGAAGGATACGTGCCCCAAGAGGAGGTCCCAGTCTATGAAAA CAAGTATGTGAAGTTTTTCAAGAGTAAACCAGAACTTCCCCCAGGGCTGAGCCCTGAGGCCACCACACCG GTCACCCCATCCAGACCTGAAGGTGGTGAGACAGGCCTCTCCAAGACAGCCAAACGAAACCTGAAGCGGA AGGAGAAGAGGCGGCAGCAACAGGAGAAAGAAGCAGAGGCCTTGAGCAGGACTCTTGATAAGGTGTCTCT GGGAGACACAGCCCAGATACCTAGTGCTCTCCAAGGCCCCCAGGCCACGCCTCTAGCTGCCTCTGATCCA TCCGACTCTGCTGCCACCACAGAGAAAGCCAAGAAGATCAAGAACCTGAGGAAGAAGCTTCGGCAGGTGG AGGAGCTGCAGCAACGCATCCAGGCCGGGGAGGTCAGCCAGCCCAGCAGAGAACAGCTGGAGAAGCTGGC TCGACGGAGGGTTCTGGAGGAGGAGCTGGAGGACTTGGAGTTGGGCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218272 representing NM_030100
Red=Cloning site Green=Tags(s) METASTPEATGTGKYIASTQRPDGTWRKQRRVKEGYVPQEEVPVYENKYVKFFKSKPELPPGLSPEATTP VTPSRPEGGETGLSKTAKRNLKRKEKRRQQQEKEAEALSRTLDKVSLGDTAQIPSALQGPQATPLAASDP SDSAATTEKAKKIKNLRKKLRQVEELQQRIQAGEVSQPSREQLEKLARRRVLEEELEDLELGL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030100 |
| ORF Size | 609 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030100.4, NP_084376.2 |
| RefSeq Size | 1186 bp |
| RefSeq ORF | 612 bp |
| Locus ID | 78428 |
| UniProt ID | Q8CHP5 |
| Gene Summary | Key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmark for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. Acts as an EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated. Interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC211880 | Wibg (untagged) - Mouse within bgcn homolog (Drosophila) (Wibg), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MR218272 | Wibg (Myc-DDK-tagged) - Mouse within bgcn homolog (Drosophila) (Wibg), transcript variant 1 |
CNY 2,000.00 |
|
| MR218272L3 | Lenti ORF clone of Wibg (Myc-DDK-tagged) - Mouse within bgcn homolog (Drosophila) (Wibg), transcript variant 1 |
CNY 3,900.00 |
|
| MR218272L4 | Lenti ORF clone of Wibg (mGFP-tagged) - Mouse within bgcn homolog (Drosophila) (Wibg), transcript variant 1 |
CNY 3,900.00 |
