United States
Germany
Japan
United Kingdom
China
Mgat4a (NM_173870) Mouse Tagged ORF Clone
CAT＃: MG216106
- TrueORF®
Mgat4a (tGFP-tagged) - Mouse mannoside acetylglucosaminyltransferase 4 isoenzyme A (Mgat4a), (10ug)
ORF Plasmid: DDK
"NM_173870" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9530018I07Rik; glcNAc-T-IVa; GnT-IVa |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG216106 representing NM_173870
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTCCGAAATGGAACTGTGGCCACTGCGCTGGTATTTGTCACGTCCTTCCTTACCCTATCCTGGT ATACCACGTGGCAAAATGGGAAAGAAAAACTAATTGCTTATCAACGAGAATTCCTTGCTCTAAAAGAGCG TCTTCGAGTGGCCGAGCATAGGATATCTCAGCGCTCCTCGGAGCTAAACACCATTGTCCAGCAGTTCCGC AGAGCTGGAGCAGAGACTAATGGAAGTAAGACAGCTCTGAGTACAATCTCAGATAATACCATAAAGCTTC TAAAAGAGTTGACAAGCAAAAAATCACTTCGAGTGCCAAGTATTTATTATCATTTGCCTCATCTATTGCA AAATGAAAGAAGCCTTCAGCCCGCCGTACAGATTGGCAGTGGAAGAACGGGAGTTTCAATAGTTATGGGA ATTCCTACTGTGAAGAGAGAAGTTAAATCTTACCTCGTAGAAACCCTTCACTCCCTTATTGATAATCTGT ATCCTGAAGAGAAGCTGGACTGTGTTATAGTCGTCTTCATAGGAGAGACAGATCTTGATTATGTTCACAG CGTTGTTGCCAACCTGGAGAAAGAATTTTCTAGAGAAATTAGTTCTGGCCTGCTGGAAATAATCTCTCCT CCTGAAAGCTATTACCCCGACTTGACAAACCTGAAGGAGACGTTCGGAGACTCCAAGGAAAGAGTGAGAT GGAGAACCAAGCAAAACCTGGATTACTGTTTTCTGATGATGTATGCTCAGGAGAAGGGCATCTACTACAT TCAGCTTGAAGACGATATTATTGTCAAACAAAACTATTTTAATACCATAAAGAATTTTGCACTTCAACTT TCTTCGGAAGAATGGATGATTCTAGAGTTTTCCCAGCTTGGCTTCATTGGAAAAATGTTCCAGGCGCCGG ACCTGGCGCTGGTCGTGGAGTTCATCCTCATGTTCTATAAGGAGAAGCCCATTGACTGGCTGCTGGACCA CATTCTCTGGGTGAAGGTCTGCAACCCCGAAAAAGATGCTAAACACTGCGACAGACAGAAGGCAAACCTA CGAATCCGCTTCCGACCCTCCCTCTTCCAGCACGTGGGCCTACACTCGTCTCTGTCGGGGAAGATTCAGA AACTCACGGATAAAGATTACATGAAGCCATTGCTTCTCAAGGTCCACGTGAACCCGCCTGCAGAGGTCTC CACCTCCCTGAAGGTGTACCAAGGGCACACCCTGGAGAAGACCTACATGGGGGAAGACTTCTTTTGGGCC ATCACCCCCACGGCTGGAGACTACATCTTGTTTAAATTTGACAAACCGGTCAACGTGGAGAGTTATTTGT TCCACAGCGGCAATCAAGAGCACCCAGGAGACATCCTGCTGAACACGACCGTGGATGTTCTCCCTCTTAA GAGCGACAGTTTGGAAATCAGCAAAGAAACCAAAGACAAACGATTAGAAGATGGCTATTTCAGAATAGGA AAATTTGAGTATGGTGTTGCAGAGGGAATTGTGGATCCTGGTCTAAACCCTATTTCAGCCTTTCGACTTT CCGTTATTCAGAACTCAGCTGTTTGGGCCATTCTTAATGAGATTCATATTAAAAAAGTCACCAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216106 representing NM_173870
Red=Cloning site Green=Tags(s) MRLRNGTVATALVFVTSFLTLSWYTTWQNGKEKLIAYQREFLALKERLRVAEHRISQRSSELNTIVQQFR RAGAETNGSKTALSTISDNTIKLLKELTSKKSLRVPSIYYHLPHLLQNERSLQPAVQIGSGRTGVSIVMG IPTVKREVKSYLVETLHSLIDNLYPEEKLDCVIVVFIGETDLDYVHSVVANLEKEFSREISSGLLEIISP PESYYPDLTNLKETFGDSKERVRWRTKQNLDYCFLMMYAQEKGIYYIQLEDDIIVKQNYFNTIKNFALQL SSEEWMILEFSQLGFIGKMFQAPDLALVVEFILMFYKEKPIDWLLDHILWVKVCNPEKDAKHCDRQKANL RIRFRPSLFQHVGLHSSLSGKIQKLTDKDYMKPLLLKVHVNPPAEVSTSLKVYQGHTLEKTYMGEDFFWA ITPTAGDYILFKFDKPVNVESYLFHSGNQEHPGDILLNTTVDVLPLKSDSLEISKETKDKRLEDGYFRIG KFEYGVAEGIVDPGLNPISAFRLSVIQNSAVWAILNEIHIKKVTS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_173870 |
| ORF Size | 1605 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_173870.3, NP_776295.1 |
| RefSeq Size | 7054 bp |
| RefSeq ORF | 1608 bp |
| Locus ID | 269181 |
| UniProt ID | Q812G0 |
| Gene Summary | Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217662 | Mgat4a (untagged) - Mouse mannoside acetylglucosaminyltransferase 4, isoenzyme A (Mgat4a), (10ug) |
CNY 6,840.00 |
|
| MR216106 | Mgat4a (Myc-DDK-tagged) - Mouse mannoside acetylglucosaminyltransferase 4, isoenzyme A (Mgat4a) |
CNY 3,984.00 |
|
| MR216106L3 | Lenti ORF clone of Mgat4a (Myc-DDK-tagged) - Mouse mannoside acetylglucosaminyltransferase 4, isoenzyme A (Mgat4a) |
CNY 6,460.00 |
|
| MR216106L4 | Lenti ORF clone of Mgat4a (mGFP-tagged) - Mouse mannoside acetylglucosaminyltransferase 4, isoenzyme A (Mgat4a) |
CNY 6,460.00 |
