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Ptprn2 (NM_011215) Mouse Tagged ORF Clone
CAT＃: MG215745
- TrueORF®
Ptprn2 (tGFP-tagged) - Mouse protein tyrosine phosphatase receptor type N polypeptide 2 (Ptprn2), (10ug)
ORF Plasmid: DDK
"NM_011215" in other vectors (4)
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CNY 9,220.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4930425H11Rik; IA2beta; mKIAA0387; phogrin; Phol; PTP-NP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG215745 representing NM_011215
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCCGCCGCTCCCGCTTTTGCTGCTGCTGCTGCTACCGCCGCCGCTGCCTCGCGCTCTGCCCGCCC CCGCGTCTGCCCGCGGCCGGCAGCTCCCGGGGCGCCTGGGATGCTTGTTTGAGGATGGCTTGTGTGGATC ACTGGAGACCTGTGTGAACGATGGTGTGTTTGGAAGATGTCAAAAGGTTCCGGTGATGGACACTTACCGA TATGAGGTACCACCAGGAGCCCTGCTGCACCTGAAGGTCACCTTACAGAAGCTCTCCCGTACAGGTTTCA CGTGGCAAGATGACTATACCCAGCGTGTGATCGCCCAGGAGCTTGCAAACCTCCCCAAGGCCTACCTATG GCATGGGGAAACGTCCGGCCCACCCAGGTCCTTACAACAGAATGCTGACAATGAAAAATGGTTCAGTCTG GAGAGGGAGGTGGCCCTGGCCAAGACCCTTCGGCGCTATCTGCCCTACCTGGAGCTTCTGTCCCAGACCC CAACAGCAAATGCACACTCTAGGATAGACCATGAGACTCGTCCAGCCAAGGGTGAAGACTCTTCCCCTGA GAACATCCTGACCTACGTGGCCCACACATCAGCACTGACCTATCCTCCTGCAACCCGGGCCAAGTATCCT GATAACCTTCTGCGGCCCTTTAGCCGGCTCCAGCCAGATGAGCTCAGCCCCAAGGTAGACGGTGACATAG ACAAACAGAAACTGATTGCAGCACTGGGCGCCTACACTGCTCAGAGGCTTCCTGGAGAAAATGACCCAGA GCCACGGTACCTTGTACATGGTTCCTCGAGAGCACCAAGGCCATTCTCAGCAACTGCTTTGTCTCAGAGA TGGCCTCCACCTCCTGGAGACGCCAAAGACTCCCCGAGTATGGATGATGACACACTCCTGCAGAGTCTCC TGAAGGATTTGCAGCAGAACTCTGAAGTGGACCGCCTGGGCCCCCTGAAGGAGGAGAAAGCAGACTCAGT TGCTGGAGCCATACAAAGTGATCCTGCAGAGGGAAGCCAAGAAAGCCACGGGAGAGGGGCTGAAGGACAG CCAAGAGAGCAGACAGATGCCCCAGAGACAATGCTTCAAGATCACAGACTATCAGAGGTGGATGACCCAG TGTACAAGGAGGTCAACCGTCTGAGCTTCCAGCTTGGGGACCTCTTGAAGGACTATGGGTCTCCTCTCTT ACCTGAAGGTCCCCTTCTAGAAAAATCCTCCAGAGAAGAGATTAAGAAGTCAGAGCAGCCAGAGGAGGTC TTGTCTTCAGAAGAGGAGACTGCTGGGGTGGAGCATGTGAGGAGCCGGACTTACTCCAAAGACCTATTTG AAAGGAAACCAAACTCAGAGCCCCAGCCCAGGAGGCTTGAGGATCAGTTCCAAAACCGAGCTCCAGAGTT GTGGGAGGATGAAGAAAGCCTCAAATTGGCAGCACAGGGACCCCCTAGTGGAGGCCTACAGCTGGAAGTG CAGCCTTCTGAGGAACAGCAGGGATACATCCTCACAGGAAACAACCCTCTAAGTCCAGAGAAGGGGAAGC AGCTGATGGACCAAGTTGCCCACATCCTCCGGGTACCTTCCAGCTTCTTTGCAGATATCAAAGTTTTGGG ACCAGCAGTGACCTTCAAAGTAAGTGCCAACATCCAAAACATGACAACTGCCGATGTCATCAAGGCTGCA GCTGACAACAAAGACCAGCTGGAGAAGGCAACTGGACTGACAATCCTTCAAAGTGGAATCAGGCCGAAGG GAAAGCTCAAACTCCTGCCGCATCAGGAAGAGCAAGAGGACTCTACCAAGTTCATTTTGCTCACCTTCCT CTCCATTGCCTGCATCCTGGGGGTTCTCCTGGCTTCCAGCCTTGCCTACTGCCTCCGCCACAACTCACAC TACAAGCTGAAGGACAAGTTGTCTGGACTAGGCGCTGACCCCAGTGCAGATGCCACTGAAGCCTACCAGG AGCTATGCCGCCAGCGTATGGCTGTTCGTCCACAGGACCGCTCTGAGGGACCACATACATCACGCATCAA CAGCGTCTCATCCCAGTTCAGCGATGGGCCGATGCCTAGTCCTTCGGCTCGGAGCAGCACTTCATCCTGG TCTGAGGAGCCTGTCCAGTCCAACATGGACATCTCTACTGGCCACATGATCCTGGCCTACATGGAAGACC ATCTGAAGAACAAGAACCGCCTGGAGAAGGAGTGGGAAGCACTGTGCGCCTACCAAGCAGAGCCCAACAG CTCACTTGTGGCCCAGAGAGAGGAGAATGCACCCAAGAACCGTTCCCTGGCTGTGCTGACCTATGACCAC TCCAGGATCCTGTTGAAGTCTCAAAACAGCCATGGCAGTTCCGACTACATCAATGCCAGCCCCATTATGG ACCATGACCCACGAAACCCCGCATACATTGCCACCCAAGGCCCACTTCCCGCCACGGTGGCCGACTTCTG GCAGATGGTGTGGGAAAGCGGCTGTGCAGTCATTGTCATGCTGACACCCCTCTCCGAGAACGGCGTCCGG CAGTGCCATCACTACTGGCCCGATGAAGGCTCCAACCTCTACCATGTCTACGAGGTCAATCTAGTCTCTG AGCACATATGGTGCCAGGATTTCCTGGTGAGAAGCTTTTACCTGAAGAACCTGCAGACCAACGAGACTCG CACGGTGACCCAGTTCCACTTCCTGAGTTGGTATGACCAGGGAGTCCCTTCCTCCACGAGGTCACTCCTG GATTTCCGCAGAAAAGTGAACAAATGCTACCGAGGCCGCTCTTGTCCGATCATTGTCCATTGCAGTGACG GCGCCGGCAGGAGTGGAACCTACGTCCTGATTGACATGGTTCTCAATAAGATGGCCAAAGGTGCTAAAGA GATTGATATCGCAGCGACCCTGGAGCACTTGAGGGACCAGAGACCAGGCATGGTCCAGACAAAGGAGCAG TTTGAGTTTGCGCTGACAGCTGTGGCTGAGGAGGTGAATGCCATCCTGAAGGCCCTTCCCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215745 representing NM_011215
Red=Cloning site Green=Tags(s) MGPPLPLLLLLLLPPPLPRALPAPASARGRQLPGRLGCLFEDGLCGSLETCVNDGVFGRCQKVPVMDTYR YEVPPGALLHLKVTLQKLSRTGFTWQDDYTQRVIAQELANLPKAYLWHGETSGPPRSLQQNADNEKWFSL EREVALAKTLRRYLPYLELLSQTPTANAHSRIDHETRPAKGEDSSPENILTYVAHTSALTYPPATRAKYP DNLLRPFSRLQPDELSPKVDGDIDKQKLIAALGAYTAQRLPGENDPEPRYLVHGSSRAPRPFSATALSQR WPPPPGDAKDSPSMDDDTLLQSLLKDLQQNSEVDRLGPLKEEKADSVAGAIQSDPAEGSQESHGRGAEGQ PREQTDAPETMLQDHRLSEVDDPVYKEVNRLSFQLGDLLKDYGSPLLPEGPLLEKSSREEIKKSEQPEEV LSSEEETAGVEHVRSRTYSKDLFERKPNSEPQPRRLEDQFQNRAPELWEDEESLKLAAQGPPSGGLQLEV QPSEEQQGYILTGNNPLSPEKGKQLMDQVAHILRVPSSFFADIKVLGPAVTFKVSANIQNMTTADVIKAA ADNKDQLEKATGLTILQSGIRPKGKLKLLPHQEEQEDSTKFILLTFLSIACILGVLLASSLAYCLRHNSH YKLKDKLSGLGADPSADATEAYQELCRQRMAVRPQDRSEGPHTSRINSVSSQFSDGPMPSPSARSSTSSW SEEPVQSNMDISTGHMILAYMEDHLKNKNRLEKEWEALCAYQAEPNSSLVAQREENAPKNRSLAVLTYDH SRILLKSQNSHGSSDYINASPIMDHDPRNPAYIATQGPLPATVADFWQMVWESGCAVIVMLTPLSENGVR QCHHYWPDEGSNLYHVYEVNLVSEHIWCQDFLVRSFYLKNLQTNETRTVTQFHFLSWYDQGVPSSTRSLL DFRRKVNKCYRGRSCPIIVHCSDGAGRSGTYVLIDMVLNKMAKGAKEIDIAATLEHLRDQRPGMVQTKEQ FEFALTAVAEEVNAILKALPQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011215 |
| ORF Size | 3003 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011215.2, NP_035345.2 |
| RefSeq Size | 4688 bp |
| RefSeq ORF | 3006 bp |
| Locus ID | 19276 |
| UniProt ID | P80560 |
| Gene Summary | Plays a role in vesicle-mediated secretory processes (PubMed:21732083). Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets. Required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation (PubMed:21732083). Plays a role in insulin secretion in response to glucose stimuli (PubMed:15220191, PubMed:16418280, PubMed:21732083). Required for normal accumulation of the neurotransmitters norepinephrine, dopamine and serotonin in the brain. In females, but not in males, required for normal accumulation and secretion of pituitary hormones, such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH) (PubMed:16269463). Required to maintain normal levels of renin expression and renin release (PubMed:19019914). May regulate catalytic active protein-tyrosine phosphatases such as PTPRA through dimerization (PubMed:12364328). Has phosphatidylinositol phosphatase activity; the PIPase activity is involved in its ability to regulate insulin secretion. Can dephosphorylate phosphatidylinositol 4,5-biphosphate (PI(4,5)P2), phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate (By similarity). Regulates PI(4,5)P2 level in the plasma membrane and localization of cofilin at the plasma membrane and thus is indirectly involved in regulation of actin dynamics related to cell migration and metastasis; upon hydrolyzation of PI(4,5)P2 cofilin is released from the plasma membrane and acts in the cytoplasm in severing F-actin filaments (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Phospholipase C-related catalytically inactive protein (PRIP) controls KIF5B-mediated insulin secretion
,Asano, S;Nemoto, T;Kitayama, T;Harada, K;Zhang, J;Harada, K;Tanida, I;Hirata, M;Kanematsu, T;,
Biol Open
,PubMed ID 24812354
[Ptprn2]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223149 | Ptprn2 (untagged) - Mouse protein tyrosine phosphatase, receptor type, N polypeptide 2 (Ptprn2), (10ug) |
CNY 12,920.00 |
|
| MR215745 | Ptprn2 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, N polypeptide 2 (Ptprn2) |
CNY 6,976.00 |
|
| MR215745L3 | Lenti ORF clone of Ptprn2 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, N polypeptide 2 (Ptprn2) |
CNY 10,260.00 |
|
| MR215745L4 | Lenti ORF clone of Ptprn2 (mGFP-tagged) - Mouse protein tyrosine phosphatase, receptor type, N polypeptide 2 (Ptprn2) |
CNY 10,260.00 |
