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Map3k13 (NM_172821) Mouse Tagged ORF Clone
CAT＃: MG214539
- TrueORF®
Map3k13 (tGFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase 13 (Map3k13), (10ug)
ORF Plasmid: DDK
"NM_172821" in other vectors (4)
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CNY 12,136.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C130026N12Rik; LZK |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG214539 representing NM_172821
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAACCCTCAGGAGCACCTGAGCTGCTCCTCTTTGCCACACTTGCCCTTGACTGAGAACAAAACCT CTGGTGGACGAAATGAGCTTGCAGCTATGGGGAACCACCCTTCACCCAAGCTCCCTGAGGACCCTCAGGA GAGGGGGGCTATACAGTCAGAGCTGATGGAGATCACAGGCAGCCCGATCTCCACAACAGTGTTGACCAGT GTAAGCGAGGATTCTAGGGGCCAGTTTGAGAACAGTGTTCTTCAGCTAAGGGAACAGGATGAGTCAGAGA TGACCCTGTCTCTGGGGAACAGCAACACTGTGGATGGAGAGAACACGAACGGACCAGAAGACATCAAGAT TCAGTTCAGCAGGTCAGGCAGCGGCAGCGGCGGGTTTCTCGAAGGACTGTTTGGATGTCTCAGGCCCGTG TGGAATATCATTGGGAAGGCATATTCCACTGATTACAAGCTGCAGCAGCAAGATACTTGGGAAGTGCCAT TTGAGGAAATCTCAGAGCTGCAATGGCTGGGTAGTGGAGCCCAAGGAGCTGTCTTCCTGGGCAAATTCCG AGCAGAGGAGGTGGCCATCAAGAAGGTGAGAGAACAAAATGAGACGGACATCAAGCATCTGAGGAAGCTG AAGCACCCTAACATCATCGCCTTCAAGGGTGTTTGCACTCAGGCCCCGTGCTACTGCATCATCATGGAGT ACTGTGCCCACGGACAGCTGTACGAGGTCCTGAGAGCTGGCCGGAAGATCACGCCCCGGCTGCTTGTGGA CTGGTCCACGGGAATCGCCAGTGGAATGAATTATCTCCATCTCCACAAGATTATACACCGTGACCTCAAG TCGCCCAATGTTTTAGTGACTCACACAGATGCAGTGAAAATTTCAGACTTTGGCACATCTAAGGAACTCA GTGATAAAAGCACCAAGATGTCCTTCGCTGGCACCGTCGCGTGGATGGCACCAGAGGTGATCCGGAATGA GCCTGTCTCGGAGAAGGTGGACATATGGTCATTTGGTGTGGTACTTTGGGAGCTGCTGACAGGGGAGATT CCCTACAAGGATGTGGACTCTTCCGCCATCATTTGGGGTGTCGGCAGCAACAGCTTGCATCTCCCAGTTC CTTCCACTTGCCCGGATGGATTCAAAATCCTTATGAAACAGACATGGCAGAGTAAGCCTCGCAACCGACC TTCTTTCAGGCAGACGCTCATGCATCTGGATATCGCGTCTGCAGATGTGCTCGCCACCCCTCAAGAAACT TACTTCAAGTCTCAGGCTGAATGGAGAGAAGAAGTGAAGAAACATTTTGAGAAAATCAAAAGTGAAGGGA CCTGTATACACCGATTAGATGAAGAACTGATTCGCCGGCGCAGGGAGGAGCTCAGGCATGCTCTGGATAT TCGGGAGCACTACGAGAGAAAGCTCGAACGGGCTAACAATCTGTACATGGAGCTGAGCGCCATCATGCTG CAGCTGGAGATGCGCGAGAAGGAGCTCCTTAAGCGAGAGCAAGCAGTGGAAAAGAAGTACCCTGGAACCT ACAAACGACACCCTGTCCGTCCAATCATCCACCCGAATGCCATGGAGAAACTCATGAAAAGGAAGGGTGT GCCTCACAAGGCTGGGGTGCAGACTAAGCGGCCAGACCTGTTGCGATCCGAAGGCATTCCTAGTACAGAA GCGGTTCCCACTGCATCCCCCCTGTCTGGAAGTCCCAAAATGTCCACCGCAAGCAGCAGAAGCCGGTACC GAAGCAAACCACGTCACCGCCGAGGGAACAGTAGAGGCAGCCATAGCGACTTCGCAGCAATCTTGAAAAC CCAGCCCGCCCAGGAAAATTCACCCCACCCCACCTACATGCACCACACTCAGGCTCAGTGCGCTTCAGTC CATCAACATAACCCTCTCCAGCAGCAATACCAGCAAATTCCTCCTGCCCAGCCTCAGAGCCGGCATCCCA GACTCAATGCGCATGGGCAGGACATTGCAACCTGCGCCAATAACCTGAGGTACTTTGGCCCCGCAGCGGC CCTGCGGAGTCCACTCAGCAACCATGCACAAAGACAGATGCCTGGCTCAAGCCCCGACCTCATCTCCACA GCCATGGCTGCAGATTGGAGAAATTCTGAACTTGATCAAGACCAAGTTGGACCCTGGGGCTGTTGTCAGG CTGAACCCTATGATCCCTGTTTCCAGTGCAGGCCAGAACATTCTGGGTCTTTAGATGTTCCCACTACTGA GCCAGTGGGGAGGAGCCCAGACCTTTCCAGTTCACCAGCACACAATCCTCTTTCAGGAAATGCCCAAGGC TCTGAGAGAACAGGGGCAAATGGATTCAGTGGCTGTCAGTCTGGTATTTCTCATCAATTCACACCCCCAA TGCTACCTCAGAAGACACGGCCCCTTCAGAAGAGTGGCGATGACTCCTCAGAAGAGGAAGGGGAAGTGGA CAGTGAAGTAGAATTTCCTCGACGACAGAGGCCTCACCGCTGCATTAGCAGTTACCAGTCATACTCAACC TTTAGCTCCGAGAACTTCTCTGTGTCTGATGGAGAGGAGGGCAACACTAGTGACCACTCGAACAGTCCCG ATGAATCCGCCAACAGACGGCAGGACCGCCTGGCAGAGACGCTGGACGACCTGCTGTCTCAGACACCCGA GGCCCCCATCGAGATCTCCTCCCACTCAGATGGGCTGTCTGACAAGGAGTGTGCTGTTCGGCGCGTGAAG ACACAGATGTCCCTAGGCAAGCTGTGTGCCGAGGAGCGCGGCTACGAGAACCCTGTGCAGTTTGGAGACT CGGACTGTGACTCTTCAGAGGGAGAGTGTTCCGATGCCACAGTTAGGACCAGCAAGAACTACAGCTCTGC CACCTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG214539 representing NM_172821
Red=Cloning site Green=Tags(s) MANPQEHLSCSSLPHLPLTENKTSGGRNELAAMGNHPSPKLPEDPQERGAIQSELMEITGSPISTTVLTS VSEDSRGQFENSVLQLREQDESEMTLSLGNSNTVDGENTNGPEDIKIQFSRSGSGSGGFLEGLFGCLRPV WNIIGKAYSTDYKLQQQDTWEVPFEEISELQWLGSGAQGAVFLGKFRAEEVAIKKVREQNETDIKHLRKL KHPNIIAFKGVCTQAPCYCIIMEYCAHGQLYEVLRAGRKITPRLLVDWSTGIASGMNYLHLHKIIHRDLK SPNVLVTHTDAVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEI PYKDVDSSAIIWGVGSNSLHLPVPSTCPDGFKILMKQTWQSKPRNRPSFRQTLMHLDIASADVLATPQET YFKSQAEWREEVKKHFEKIKSEGTCIHRLDEELIRRRREELRHALDIREHYERKLERANNLYMELSAIML QLEMREKELLKREQAVEKKYPGTYKRHPVRPIIHPNAMEKLMKRKGVPHKAGVQTKRPDLLRSEGIPSTE AVPTASPLSGSPKMSTASSRSRYRSKPRHRRGNSRGSHSDFAAILKTQPAQENSPHPTYMHHTQAQCASV HQHNPLQQQYQQIPPAQPQSRHPRLNAHGQDIATCANNLRYFGPAAALRSPLSNHAQRQMPGSSPDLIST AMAADWRNSELDQDQVGPWGCCQAEPYDPCFQCRPEHSGSLDVPTTEPVGRSPDLSSSPAHNPLSGNAQG SERTGANGFSGCQSGISHQFTPPMLPQKTRPLQKSGDDSSEEEGEVDSEVEFPRRQRPHRCISSYQSYST FSSENFSVSDGEEGNTSDHSNSPDESANRRQDRLAETLDDLLSQTPEAPIEISSHSDGLSDKECAVRRVK TQMSLGKLCAEERGYENPVQFGDSDCDSSEGECSDATVRTSKNYSSATW TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172821 |
| ORF Size | 2877 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172821.3, NP_766409.2 |
| RefSeq Size | 6709 bp |
| RefSeq ORF | 2880 bp |
| Locus ID | 71751 |
| UniProt ID | Q1HKZ5 |
| Gene Summary | Activates the JUN N-terminal pathway through activation of the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the IKBKB-containing complex that phosphorylates inhibitors of NF-kappa-B (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222837 | Map3k13 (untagged) - Mouse mitogen-activated protein kinase kinase kinase 13 (Map3k13), (10ug) |
CNY 12,260.00 |
|
| MR214539 | Map3k13 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase 13 (Map3k13) |
CNY 10,536.00 |
|
| MR214539L3 | Lenti ORF clone of Map3k13 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase 13 (Map3k13) |
CNY 11,500.00 |
|
| MR214539L4 | Lenti ORF clone of Map3k13 (mGFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase 13 (Map3k13) |
CNY 11,500.00 |
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