Chadl (NM_001164320) Mouse Tagged ORF Clone

CAT＃: MG213896

TrueORF^®

Chadl (tGFP-tagged) - Mouse chondroadherin-like (Chadl), (10ug)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_001164320" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 7,220.00

货期*

3周

规格

Product images

经常一起买 (4)

PS100010

pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug

CNY 6,080.00

TA150041

Mouse monoclonal turboGFP antibody, clone OTI2H8

CNY 1,000.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AY100452; D930017K21Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG213896 representing NM_001164320 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGGCCCCAGAGCTCCATCTGGGTCTTTATGCTGTTGCTGTTCATGGTGTTGCTCCAGAGCCCAG CCTGGCACGTAGCTGCCCAGCGATGCCCACAGACCTGTGTCTGCGACAACTCAAGACGGCATGTTACCTG CCGGCACCAGAACCTCACCGAGGTGCCAAACACCATCCCTGAGCTGACCCAGAGGCTGGACCTCCAGGGC AATATACTGAAGGTCCTCCCTGCAGCGGCCTTCCAGGACCTGCCCCACCTCACGCATCTGGACCTGCGGA ACTGTCAGGTGGAGATGGTGGCCGAGGGTGCCTTCCGTGGCCTGGGTCGCCTGCTCCTCCTCAACCTGGC TTCCAACCGCCTGAGCACCCTGCCCCAGGAGGCGCTGGACGGGCTGGGCTCGCTGAGGCGCCTGGAGCTG GAGGGGAACATGTTGGAGGAGCTGCGCCCGGGGACCTTCGGCGCCCTGGGCTCGCTGACCACTCTCAACT TGGCCCACAACGCCCTGGTCTACCTGCCCGCCATGGCCTTCCAGGGGCTGCTGCGCACCCGCTGGCTGCA GCTGTCTCACAATGCGCTCAGCGTCCTGGCCCCCGAGGCTCTGGCCGGCCTGCCGGCGCTGCGCCGTCTC AGCCTGCACCACAACGAGCTGCAGGCCCTGCCCGGGGCCGCCCTGTCCCAGGCCCGCAGCCTGGCGCGGC TGGAGCTGGGTCACAACCCGCTCACCTACACGGGAGAGGAGGACGGCCTGGCGCTGCCCGGCCTGCGGGA GCTCGCACTGGACCACGGGTCCCTGCAGGCCCTGGGGCCCCGGGCCTTCGCCCACTGCCCGCGCCTGCAC ACCCTCGACCTCCGCGGGAACCAGCTCACCACCCTGCCCCCGCTACAGGTCCCCGGCCAGCTGCGCCGCC TGCGGCTGCAGGGCAACCCGCTGTGGTGCGCCTGCCACGCACGGCCCCTGCTCGAGTGGCTGGTGCGCGC TCGGGTGCGCTCGGATGGCGCGTGCCGGGGCCCGCGGCGCCTGCGAGGCGAGGCCCTGGATACGCTGCGG CCCTCTGACCTGCGCTGCCCGGGCGACGCGGCGGCGGGGGACGGGGATGGGGACGAGGACGAGGACCGGC CGGCCGGTCCCCGCGCTCCTCCTCTTCGCTCCCCGCACGGAGAGGCCGCGTGGGCCACGCCCTGCCCTCC AGCCTGCGCGTGCGTCGCCGAGACCCGGCACAGCACTTGCGACGGCCGCGGCCTGCAGGCCGTGCCCCGC GGCTTCCCCAACGACACCCAGCTCCTGGACCTGAGGCGCAACCACTTCCCCTCGGTGCCCCGCGCGGCCT TCCCGGGCTTGCGCCACTTGGTGTCGCTGCACCTGCAGCACTGCGGCGTCGCGGAGCTGGAGCCCGGCGC CTTGGCCGGCCTGGACCGCCTGCTCTATCTCTACCTCTCCCACAACCAGCTCTCGGGCCTGAGCGCCGCC GCCCTCGAAGGGGCCCCCAACCTGGGCTACCTGTACCTGGAGCACAACCGCTTCCTGAGGATCCCGGGGA CCGCTCTGCGGGCCCTACCCACCCTTGTCTCGCTACACCTCCAGGACAACGCGGTGGACCGCCTGGCGCC CGGGGATCTGGCAGGAGCGCGGGCTTTGCGCTGCCTTTACCTGAGTGGCAATCACATCACCCAGGTTTCA CCCGGGGCGCTCGGTCCAGCGCGGGAGCTGGAGAAGCTGCACCTCGACAGAAACCGGCTGCGAGAGGTGC CGACTGGAGCCTTGGAGGGGCTGCCGGCGCTCAAGGAGCTGCAGCTCTCGGGGAACCCGCTCCGGGCTCT TCCAGACGGCGCCTTTCAGCCCGTGGGGCGGTCGCTGCAGCAGCTCTTCCTGAATAGCAGTGACCTGGAG CAGATTTCTCCCAGGGCCTTCTCAGGCCTGGGAAAAGGGCTGCGGAGCTTGTACCTGCACAAGAATCAAC TTCAGTCCCTGCCTGCCCCGCTGGGGCTCAGCGGGCTGGAGCTGGTCGACCTCAGCGGTAATCCTTTCCA CTGTGACTGCCAGCTGCTCCCGCTGCACAGGTGGCTCACTGGGCTGAACCTGCGGGTGGGTGCCACCTGT GCCACCCCTCCCAGTGTCCGTGGCCAGAAGGTGAAGGTTGCAGCTCCTGTCTTTGAAGCCTGCCCAGGCT GGACTGCCAGGAAGGCCAAGAGGACACCAACCTCCAGAGGCAGTGCCAGGAGAACTCCCAGCCTCAGCCG ACAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG213896 representing NM_001164320 Red=Cloning site Green=Tags(s) MERPQSSIWVFMLLLFMVLLQSPAWHVAAQRCPQTCVCDNSRRHVTCRHQNLTEVPNTIPELTQRLDLQG NILKVLPAAAFQDLPHLTHLDLRNCQVEMVAEGAFRGLGRLLLLNLASNRLSTLPQEALDGLGSLRRLEL EGNMLEELRPGTFGALGSLTTLNLAHNALVYLPAMAFQGLLRTRWLQLSHNALSVLAPEALAGLPALRRL SLHHNELQALPGAALSQARSLARLELGHNPLTYTGEEDGLALPGLRELALDHGSLQALGPRAFAHCPRLH TLDLRGNQLTTLPPLQVPGQLRRLRLQGNPLWCACHARPLLEWLVRARVRSDGACRGPRRLRGEALDTLR PSDLRCPGDAAAGDGDGDEDEDRPAGPRAPPLRSPHGEAAWATPCPPACACVAETRHSTCDGRGLQAVPR GFPNDTQLLDLRRNHFPSVPRAAFPGLRHLVSLHLQHCGVAELEPGALAGLDRLLYLYLSHNQLSGLSAA ALEGAPNLGYLYLEHNRFLRIPGTALRALPTLVSLHLQDNAVDRLAPGDLAGARALRCLYLSGNHITQVS PGALGPARELEKLHLDRNRLREVPTGALEGLPALKELQLSGNPLRALPDGAFQPVGRSLQQLFLNSSDLE QISPRAFSGLGKGLRSLYLHKNQLQSLPAPLGLSGLELVDLSGNPFHCDCQLLPLHRWLTGLNLRVGATC ATPPSVRGQKVKVAAPVFEACPGWTARKAKRTPTSRGSARRTPSLSRH TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_001164320
ORF Size	2244 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001164320.1, NP_001157792.1
RefSeq Size	2547 bp
RefSeq ORF	2247 bp
Locus ID	214685
UniProt ID	E9Q7T7
Gene Summary	Potential negative modulator of chondrocyte differentiation. Inhibits collagen fibrillogenesis in vitro. May influence chondrocyte's differentiation by acting on its cellular collagenous microenvironment.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MC221326	Chadl (untagged) - Mouse chondroadherin-like (Chadl), (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,690.00
MR213896	Chadl (Myc-DDK-tagged) - Mouse chondroadherin-like (Chadl)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,480.00 CNY 6,560.00
MR213896L3	Lenti ORF clone of Chadl (Myc-DDK-tagged) - Mouse chondroadherin-like (Chadl)	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,460.00
MR213896L4	Lenti ORF clone of Chadl (mGFP-tagged) - Mouse chondroadherin-like (Chadl)	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,460.00

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