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Myh10 (NM_175260) Mouse Tagged ORF Clone
CAT＃: MG212106
- TrueORF®
Myh10 (tGFP-tagged) - Mouse myosin, heavy polypeptide 10, non-muscle (Myh10)
ORF Plasmid: DDK
"NM_175260" in other vectors (2)
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CNY 23,940.00
货期*
3周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 5730504C04Rik; 9330167F11Rik; Fltn; mKIAA3005; Myhn-2; Myhn2; NMHC-B; NMHC II-B; NMHCII-B |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG212106 representing NM_175260
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGAGAACTGGACTGGAGGATCCCGAGAGGTATCTCTTTGTGGACAGGGCTGTCATCTACAACC CTGCCACTCAAGCTGACTGGACAGCTAAAAAGCTGGTGTGGATTCCATCGGAACGCCATGGTTTTGAGGC AGCTAGTATTAAAGAAGAGCGGGGCGATGAGGTTATGGTGGAGCTGGCAGAGAATGGGAAGAAAGCAATG GTCAACAAAGATGACATTCAGAAGATGAACCCACCAAAGTTCTCCAAGGTGGAGGATATGGCAGAGCTGA CATGCTTGAACGAAGCCTCTGTCTTACATAATTTGAAGGACCGCTACTATTCAGGACTTATCTATACTTA CTCTGGACTCTTCTGTGTGGTGATAAATCCTTACAAGAACCTTCCAATTTACTCTGAGAATATTATTGAA ATGTATAGAGGGAAGAAACGCCATGAGATGCCACCACACATCTACGCCATATCAGAGTCTGCTTACAGAT GCATGCTTCAAGATCGTGAGGACCAGTCAATTCTATGCACGGGTGAATCGGGTGCCGGGAAGACAGAAAA TACCAAGAAAGTCATTCAGTACCTTGCCCACGTTGCTTCTTCTCACAAAGGAAGAAAGGACCATAATATT CCTGGGGAACTTGAACGGCAGCTTTTACAAGCAAATCCAATTCTGGAATCCTTTGGAAATGCGAAGACTG TGAAAAATGATAACTCATCTCGCTTTGGCAAGTTTATCCGGATCAACTTTGATGTAACTGGCTATATTGT TGGGGCCAACATTGAAACATACCTTCTGGAAAAGTCTCGTGCTGTTCGTCAAGCTAAAGATGAGCGTACA TTTCATATCTTTTATCAGTTGCTCTCTGGAGCAGGGGAACACCTGAAATCCGACTTACTCCTGGAAGGTT TCAACAACTACAGATTCCTCTCCAATGGCTATATTCCTATTCCTGGACAGCAAGACAAGGATAACTTCCA GGAGACCATGGAAGCCATGCACATCATGGGCTTCTCTCACGAAGAGATCCTCTCAATGCTTAAAGTCGTA TCTTCAGTGCTGCAGTTTGGAAACATCTCTTTCAAAAAGGAGAGAAACACTGACCAAGCCTCCATGCCGG AGAACACAGTCGCACAGAAGCTCTGCCACCTGCTCGGGATGAATGTGATGGAGTTCACTCGGGCTATCCT CACGCCCAGGATCAAGGTTGGCCGGGATTACGTACAGAAAGCCCAGACCAAAGAGCAGGCTGATTTTGCA GTGGAAGCATTGGCAAAAGCTACCTATGAGCGGTTGTTTCGCTGGCTCGTTCACCGCATCAATAAAGCGC TGGATAGGACCAAACGCCAGGGAGCTTCCTTCATTGGGATCCTGGATATTGCTGGTTTTGAAATTTTTGA GCTGAACTCCTTCGAGCAGCTGTGCATCAACTACACCAACGAGAAGCTGCAGCAGCTGTTCAACCACACC ATGTTCATCCTGGAGCAGGAGGAGTACCAGCGAGAGGGCATCGAGTGGAACTTTATCGACTTCGGCCTGG ACCTGCAGCCCTGCATCGACCTGATAGAGAGACCTGCCAATCCCCCTGGCGTGCTGGCCCTCCTGGATGA AGAATGCTGGTTCCCCAAAGCTACAGATAAAACATTTGTTGAAAAGCTGGTTCAGGAGCAAGGTTCCCAC TCCAAGTTTCAGAAGCCGCGCCAACTGAAAGACAAAGCCGACTTCTGCATCATCCACTACGCGGGGAAGG TGGACTATAAGGCAGATGAGTGGCTGATGAAGAACATGGACCCGCTGAATGACAACGTGGCCACCCTCCT GCACCAGTCCTCGGACAGATTTGTGGCTGAGCTTTGGAAGGACGTGGACCGAATTGTAGGTCTGGATCAA GTCACTGGGATGACTGAGACCGCGTTTGGCTCTGCATACAAAACCAAGAAGGGCATGTTCCGAACCGTCG GGCAGCTCTACAAGGAGTCTCTCACCAAGCTGATGGCAACTCTCCGCAACACCAACCCCAACTTCGTCCG CTGCATCATTCCAAATCACGAGAAGCGGGCTGGGAAACTGGACCCGCACCTCGTGCTCGATCAGCTTCGC TGTAACGGCGTCCTGGAAGGGATCCGGATCTGTCGCCAGGGGTTCCCCAACCGGATAGTTTTCCAGGAAT TCAGACAGAGATATGAGATCCTAACTCCCAATGCTATTCCTAAAGGCTTCATGGATGGCAAACAGGCGTG TGAGCGAATGATCCGAGCTTTAGAACTGGACCCAAACCTGTATAGAATTGGACAGAGCAAGATATTTTTC CGAGCTGGAGTTTTGGCGCACTTAGAAGAAGAAAGAGATTTAAAAATCACTGATATCATCATCTTTTTCC AAGCTGTATGCAGAGGCTACCTCGCCCGAAAGGCCTTTGCCAAGAAACAGCAACAACTAAGTGCCTTAAA GGTCTTGCAGCGGAACTGTGCGGCGTACCTGAAGCTGCGACACTGGCAGTGGTGGCGTGTCTTCACGAAG GTGAAGCCTCTCCTCCAAGTGACCCGCCAGGAGGAAGAACTCCAGGCAAAAGATGAGGAGCTGCTGAAGG TGAAAGAGAAGCAGACAAAAGTGGAAGGGGAGCTTGAGGAGATGGAGCGGAAGCACCAGCAGCTGCTGGA AGAGAAGAATATCCTGGCAGAACAACTGCAAGCCGAGACCGAGCTCTTCGCTGAAGCAGAAGAGATGAGA GCAAGGCTTGCTGCCAAAAAGCAGGAACTGGAGGAGATTCTCCATGACCTCGAGTCCAGGGTGGAGGAGG AGGAAGAGCGGAACCAGATCCTACAGAATGAGAAGAAGAAGATGCAGGCGCACATTCAGGACCTAGAAGA ACAACTGGATGAGGAGGAGGGGGCCCGGCAAAAGCTGCAGCTGGAGAAGGTGACAGCAGAGGCTAAAATC AAGAAGATGGAAGAGGAGGTTCTGCTTCTCGAAGACCAGAATTCCAAATTTATCAAAGAAAAGAAACTCA TGGAAGACCGAATTGCTGAGTGTTCCTCTCAGCTGGCTGAAGAGGAAGAAAAGGCAAAAAACTTGGCCAA AATCAGGAATAAGCAAGAAGTGATGATCTCGGACTTAGAAGAACGCTTGAAGAAGGAGGAGAAAACTCGA CAGGAACTGGAAAAGGCCAAACGGAAGCTGGATGGGGAAACAACCGATCTGCAGGACCAGATCGCTGAGC TGCAGGCACAGGTCGATGAGCTCAAAGTCCAGTTGACCAAGAAGGAGGAGGAGCTTCAGGGGGCGCTGGC CAGAGGAGATGATGAGACACTGCACAAGAATAATGCACTTAAAGTTGCACGGGAGCTGCAGGCCCAAATC GCAGAGCTCCAGGAAGACTTTGAGTCTGAAAAGGCTTCAAGGAACAAGGCTGAGAAACAAAAACGGGACT TGAGTGAGGAGCTGGAAGCTCTGAAGACAGAGCTGGAGGACACCCTAGACACCACAGCAGCTCAGCAGGA ACTCCGCACAAAACGTGAGCAGGAAGTGGCAGAGCTGAAGAAGGCTCTTGAGGATGAAACTAAGAACCAC GAAGCTCAGATCCAGGACATGAGACAGAGGCATGCCACAGCGCTGGAGGAGCTTTCCGAGCAGCTGGAGC AAGCGAAAAGGTTCAAAGCCAACCTGGAGAAGAACAAACAGGGCCTGGAGACAGACAACAAGGAGCTGGC GTGTGAGGTGAAGGTGCTGCAGCAGGTGAAGGCGGAGTCAGAGCACAAGAGGAAGAAGCTGGATGCCCAG GTCCAGGAGCTCCATGCCAAGGTGTCAGAGGGTGACAGGCTCAGGGTAGAGCTGGCCGAGAAAGCAAACA AGCTACAGAATGAGCTGGATAATGTGTCAACCCTGCTGGAAGAAGCTGAGAAGAAAGGTATTAAGTTTGC GAAGGATGCAGCTGGTCTCGAGTCTCAACTACAGGACACACAGGAGCTCCTTCAGGAAGAGACACGGCAG AAACTGAACCTGAGCAGTCGGATCCGGCAGCTGGAGGAGGAGAAGAACAGCCTTCAGGAGCAGCAGGAGG AGGAGGAGGAGGCCAGGAAGAACCTGGAGAAGCAGGTGTTGGCTCTGCAGTCCCAGCTGGCTGACACCAA GAAGAAAGTGGACGATGACCTGGGGACAATCGAGAGTTTGGAGGAAGCCAAAAAGAAACTGCTCAAGGAT GTGGAGGCGCTGAGCCAGCGGCTGGAGGAGAAGGTCCTGGCGTATGACAAGCTGGAGAAGACCAAGAACC GGCTGCAACAAGAACTGGATGACCTGACGGTGGACCTGGACCACCAGCGCCAGATCGTCTCCAACTTGGA GAAGAAACAGAAGAAGTTCGACCAGCTGTTGGCAGAAGAAAAGGGCATCTCTGCTCGCTATGCAGAAGAG CGGGACCGGGCTGAAGCTGAGGCCAGAGAGAAAGAAACCAAAGCGCTCTCCCTGGCGCGGGCCCTTGAGG AGGCCTTGGAGGCGAAGGAGGAATTCGAGAGGCAGAACAAGCAGCTTCGAGCAGACATGGAAGACCTGAT GAGCTCTAAAGACGATGTGGGGAAGAACGTCCACGAGCTTGAGAAATCCAAGCGAGCCTTGGAGCAGCAG GTGGAGGAGATGCGGACCCAGCTGGAGGAGCTGGAGGACGAGCTGCAGGCCACTGAGGATGCCAAGCTCC GCCTGGAAGTCAACATGCAGGCCATGAAGGCCCAGTTTGAGAGGGACCTGCAAACCCGAGATGAGCAGAA TGAAGAAAAGAAGCGGCTGCTGCTTAAGCAGGTGCGGGAGCTCGAGGCAGAGCTGGAGGATGAGCGGAAA CAGCGGGCACTGGCTGTGGCGTCAAAGAAGAAGATGGAGATAGACCTGAAGGACCTGGAGGCTCAGATCG AGGCTGCGAACAAAGCCCGGGATGAAGTGATCAAGCAGCTTCGCAAACTTCAGGCACAGATGAAGGATTA CCAGCGTGAACTAGAAGAGGCTCGAGCATCTAGAGATGAGATTTTTGCTCAATCCAAAGAAAGTGAAAAG AAACTGAAGAGTCTAGAAGCAGAAATTCTTCAGCTGCAAGAGGAGCTGGCCTCATCCGAGCGAGCCCGCC GACACGCAGAGCAGGAGCGAGACGAGCTGGCTGATGAGATCGCCAACAGCGCCTCTGGAAAGTCTGCGCT GTTGGATGAGAAGCGGCGCCTGGAAGCGCGGATCGCACAGCTGGAAGAGGAGCTGGAGGAGGAGCAGAGC AACATGGAGCTGCTCAATGACCGCTTCCGCAAGACCACGCTGCAGGTGGACACACTGAACACAGAGCTGG CAGCAGAGCGCAGCGCTGCCCAGAAGAGTGACAATGCCCGCCAGCAGCTGGAGCGACAAAACAAGGAGCT GAAGGCCAAGCTGCAGGAGCTGGAGGGGGCAGTCAAGTCCAAGTTCAAGGCTACCATCTCAGCCCTGGAA GCCAAGATTGGGCAGCTGGAGGAGCAGCTTGAGCAGGAAGCCAAGGAGCGAGCAGCTGCCAACAAACTAG TCCGTCGAACAGAGAAGAAACTGAAAGAAATCTTCATGCAGGTTGAAGACGAGCGTCGGCATGCGGATCA GTATAAGGAGCAGATGGAGAAGGCTAATGCCAGGATGAAGCAGCTTAAACGACAGTTGGAAGAGGCTGAG GAAGAGGCCACACGTGCCAACGCATCTCGGCGTAAACTCCAAAGGGAGCTGGACGACGCCACTGAGGCCA ATGAAGGCCTGAGCCGCGAGGTCAGCACTCTCAAGAACCGGCTCAGGCGGGGCGGTCCAATCAGCTTTTC TTCAAGCCGATCTGGCCGGCGCCAGCTGCACATTGAGGGGGCATCGCTAGAGCTGTCAGATGACGACACA GAAAGTAAGACCAGTGATGTCAATGACACACAGCCACCCCAATCAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG212106 representing NM_175260
Red=Cloning site Green=Tags(s) MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHGFEAASIKEERGDEVMVELAENGKKAM VNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIE MYRGKKRHEMPPHIYAISESAYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNI PGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAVRQAKDERT FHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKDNFQETMEAMHIMGFSHEEILSMLKVV SSVLQFGNISFKKERNTDQASMPENTVAQKLCHLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFA VEALAKATYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHT MFILEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQEQGSH SKFQKPRQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRFVAELWKDVDRIVGLDQ VTGMTETAFGSAYKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLR CNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSKIFF RAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAAYLKLRHWQWWRVFTK VKPLLQVTRQEEELQAKDEELLKVKEKQTKVEGELEEMERKHQQLLEEKNILAEQLQAETELFAEAEEMR ARLAAKKQELEEILHDLESRVEEEEERNQILQNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKI KKMEEEVLLLEDQNSKFIKEKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTR QELEKAKRKLDGETTDLQDQIAELQAQVDELKVQLTKKEEELQGALARGDDETLHKNNALKVARELQAQI AELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKREQEVAELKKALEDETKNH EAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLETDNKELACEVKVLQQVKAESEHKRKKLDAQ VQELHAKVSEGDRLRVELAEKANKLQNELDNVSTLLEEAEKKGIKFAKDAAGLESQLQDTQELLQEETRQ KLNLSSRIRQLEEEKNSLQEQQEEEEEARKNLEKQVLALQSQLADTKKKVDDDLGTIESLEEAKKKLLKD VEALSQRLEEKVLAYDKLEKTKNRLQQELDDLTVDLDHQRQIVSNLEKKQKKFDQLLAEEKGISARYAEE RDRAEAEAREKETKALSLARALEEALEAKEEFERQNKQLRADMEDLMSSKDDVGKNVHELEKSKRALEQQ VEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERDLQTRDEQNEEKKRLLLKQVRELEAELEDERK QRALAVASKKKMEIDLKDLEAQIEAANKARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEK KLKSLEAEILQLQEELASSERARRHAEQERDELADEIANSASGKSALLDEKRRLEARIAQLEEELEEEQS NMELLNDRFRKTTLQVDTLNTELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSKFKATISALE AKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYKEQMEKANARMKQLKRQLEEAE EEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHIEGASLELSDDDT ESKTSDVNDTQPPQSE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175260 |
| ORF Size | 5928 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175260.2, NP_780469.1 |
| RefSeq Size | 7783 bp |
| RefSeq ORF | 5931 bp |
| Locus ID | 77579 |
| UniProt ID | Q61879 |
| Gene Summary | Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension; this function is mechanically antagonized by MYH9 (By similarity). Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.[UniProtKB/Swiss-Prot Function] |
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