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Smc3 (NM_007790) Mouse Tagged ORF Clone
CAT＃: MG211814
- TrueORF®
Smc3 (tGFP-tagged) - Mouse structural maintenace of chromosomes 3 (Smc3)
ORF Plasmid: DDK
"NM_007790" in other vectors (4)
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CNY 13,400.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Bamacan; Cspg6; HCAP; Mmip1; SMC-3; SmcD |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211814 representing NM_007790
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACATCAAGCAGGTGATCATCCAGGGCTTCCGAAGTTACCGAGACCAAACAATTGTAGATCCCTTCA GCTCCAAACATAATGTCATCGTGGGCAGAAATGGCTCTGGAAAAAGCAACTTCTTTTATGCAATCCAGTT TGTTCTCAGTGATGAATTCAGCCATCTTCGTCCAGAGCAGCGATTGGCTTTGTTGCATGAGGGTACAGGT CCTCGTGTTATTTCTGCTTTTGTGGAAATCATTTTTGACAATTCGGACAACCGGTTACCAATTGATAAAG AGGAAGTTTCACTTCGAAGAGTTATTGGTGCCAAAAAGGATCAGTATTTCCTAGATAAGAAGATGGTCAC GAAAAATGATGTGATGAATCTCCTTGAAAGTGCTGGGTTTTCCAGAAGTAATCCTTATTACATTGTTAAA CAAGGAAAGATCAACCAAATGGCAACGGCACCAGATTCTCAGAGATTAAAGCTGTTGAGAGAAGTAGCTG GTACTAGAGTGTATGATGAACGGAAAGAAGAAAGCATCTCCCTGATGAAAGAAACAGAGGGCAAACGGGA AAAGATCAATGAGTTGTTGAAGTACATTGAAGAGCGCCTGCACACTCTAGAGGAGGAGAAGGAGGAGCTG GCCCAGTATCAGAAGTGGGATAAGATGAGGCGCGCCCTGGAGTACACCATCTACAACCAGGAGCTCAACG AGACGCGCGCTAAGCTCGACGAGCTTTCTGCTAAGCGAGAAACGAGTGGAGAGAAATCCAGACAACTAAG GGATGCCCAGCAGGATGCAAGAGACAAAATGGAGGATATTGAGCGCCAGGTTAGAGAACTGAAAACAAAA ATTTCAGCCATGAAAGAAGAAAAAGAACAGCTTAGTGCTGAAAGACAAGAGCAGATTAAGCAAAGGACCA AGTTGGAGCTTAAAGCCAAGGATTTACAAGATGAGCTGGCGGGCAACAGTGAACAGCGGAAACGTTTATT AAAAGAGAGGCAGAAGCTGCTTGAAAAAATAGAAGAAAAGCAGAAAGAACTGGCAGAAACAGAACCTAAA TTCAACAGCGTAAAAGAAAAAGAAGAGCGAGGAATTGCGAGGTTGGCTCAAGCTACACAGGAAAGAACTG ATCTTTATGCAAAGCAGGGTCGAGGAAGCCAGTTTACGTCAAAAGAAGAAAGGGATAAATGGATTAAAAA GGAACTGAAGTCTTTAGATCAGGCAATTAATGATAAGAAAAGACAGATTGCTGCTATTCACAAGGATTTG GAGGATACCGAGGCAAATAAAGAGAAAAATCTGGAGCAATATAATAAATTGGATCAGGATCTCAATGAAG TCAAAGCTCGAGTTGAAGAACTGGACAGAAAATATTATGAAGTAAAAAATAAGAAAGATGAACTACAAAG TGAAAGAAATTACTTGTGGAGAGAGGAGAATGCAGAACAACAAGCACTTGCTGCTAAAAGAGAAGACCTT GAGAAGAAGCAGCAACTTCTTAGAGCGGCAACAGGAAAGGCCATTTTAAATGGAATAGATAGCATTAACA AAGTGCTAGAACATTTTCGGCGAAAAGGTATAAACCAGCATGTTCAAAATGGCTACCATGGCATCGTAAT GAATAACTTTGAGTGTGAACCAGCTTTCTATACTTGTGTGGAAGTCACTGCTGGTAACAGGTTATTTTAT CACATTGTTGATTCAGATGAAGTCAGCACGAAAATTTTAATGGAGTTCAATAAAATGAATCTTCCTGGAG AGGTGACTTTCCTGCCTCTTAACAAGTTAGATGTGAGAGATACTGCCTATCCTGAAACTAACGATGCTAT TCCTATGATCAGTAAGCTGAGGTACAATCCCAGATTTGACAAAGCTTTCAAACATGTATTTGGAAAGACA CTCATCTGTCGGAGCATGGAAGTTTCAACTCAGCTGGCCCGGGCCTTCACTATGGACTGTATTACTTTGG AAGGTGATCAAGTCAGCCATCGAGGTGCTCTGACTGGAGGTTATTACGACACAAGAAAGTCTCGACTTGA GTTACAGAAAGACGTTAGGAAGGCAGAGGAGGAGCTGGGTGAGCTGGAGGCTAAGCTCAATGAAAACCTA CGCAGGAACATTGAAAGGATTAATAATGAAATTGACCAGTTGATGAACCAAATGCAGCAGATAGAGACCC AACAAAGAAAATTTAAAGCATCCAGAGATAGCATATTATCAGAGATGAAGATGCTAAAAGAGAAGAGACA GCAATCAGAAAAGACCTTCATGCCAAAGCAACGTAGCTTACAAAGCTTGGAGGCAAGTCTGCATGCTATG GAGTCCACCAGAGAGTCACTGAAAGCGGAGCTAGGAACGGATTTGCTTTCTCAACTCAGTCTGGAAGATC AGAAAAGAGTCGACGCTCTGAATGATGAAATCCGTCAGCTGCAGCAGGAAAACAGACAGCTGCTAAATGA AAGAATTAAACTAGAAGGCATTATTACTCGAGTAGAGACTTACCTGAATGAGAATCTGAGGAAACGCTTG GACCAAGTAGAACAGGAACTTAATGAACTGAGAGAGACAGAAGGTGGTACTGTTCTTACTGCCACAACAT CAGAACTTGAAGCTATTAATAAAAGAGTAAAAGATACTATGGCAAGATCAGAAGATTTGGACAATTCCAT TGACAAAACAGAAGCTGGAATTAAAGAGCTCCAGAAAAGTATGGAGCGCTGGAAAAATATGGAGAAAGAA CACATGGATGCCATAAATCATGATACTAAAGAGCTGGAGAAGATGACCAACCGGCAAGGCATGCTGTTGA AGAAGAAGGAGGAGTGTATGAAGAAGATCCGGGAGCTGGGGTCCCTCCCCCAGGAAGCGTTTGAGAAGTA CCAGACACTGAGCCTGAAGCAGTTGTTTCGAAAACTGGAGCAATGCAACACAGAGTTAAAGAAGTACAGC CACGTGAACAAAAAGGCTCTAGATCAGTTTGTGAATTTCTCTGAGCAGAAGGAAAAGCTGATAAAGCGGC AAGAGGAATTGGATAGAGGCTACAAATCAATCATGGAATTGATGAATGTACTTGAACTTAGAAAATATGA AGCTATTCAGTTAACTTTCAAACAGGTATCTAAGAACTTCAGTGAAGTTTTCCAGAAGTTGGTACCTGGT GGCAAAGCGACTTTGGTGATGAAGAAAGGAGATGTGGAGGGCAGCCAGTCTCAGGACGAAGGAGAAGGGA GTGGCGAGAGTGAGCGGGGCTCTGGGTCACAGAGCAGTGTTCCATCAGTTGACCAGTTCACAGGAGTTGG GATCAGGGTGTCATTTACAGGGAAGCAAGGGGAGATGAGAGAAATGCAGCAGCTCTCAGGAGGACAGAAA TCTCTGGTAGCCCTTGCTCTCATCTTTGCCATTCAGAAATGCGACCCTGCTCCTTTTTACCTGTTTGATG AGATCGACCAAGCTTTGGATGCTCAGCACAGAAAAGCCGTGTCAGATATGATTATGGAGCTAGCCGTGCA TGCCCAGTTTATTACCACTACTTTCAGGCCTGAACTGCTTGAGTCAGCTGACAAATTCTATGGTGTAAAG TTCAGAAATAAGGTTAGTCACATTGATGTGATCACAGCAGAGATGGCCAAAGATTTTGTAGAAGACGATA CCACGCATGGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211814 representing NM_007790
Red=Cloning site Green=Tags(s) MYIKQVIIQGFRSYRDQTIVDPFSSKHNVIVGRNGSGKSNFFYAIQFVLSDEFSHLRPEQRLALLHEGTG PRVISAFVEIIFDNSDNRLPIDKEEVSLRRVIGAKKDQYFLDKKMVTKNDVMNLLESAGFSRSNPYYIVK QGKINQMATAPDSQRLKLLREVAGTRVYDERKEESISLMKETEGKREKINELLKYIEERLHTLEEEKEEL AQYQKWDKMRRALEYTIYNQELNETRAKLDELSAKRETSGEKSRQLRDAQQDARDKMEDIERQVRELKTK ISAMKEEKEQLSAERQEQIKQRTKLELKAKDLQDELAGNSEQRKRLLKERQKLLEKIEEKQKELAETEPK FNSVKEKEERGIARLAQATQERTDLYAKQGRGSQFTSKEERDKWIKKELKSLDQAINDKKRQIAAIHKDL EDTEANKEKNLEQYNKLDQDLNEVKARVEELDRKYYEVKNKKDELQSERNYLWREENAEQQALAAKREDL EKKQQLLRAATGKAILNGIDSINKVLEHFRRKGINQHVQNGYHGIVMNNFECEPAFYTCVEVTAGNRLFY HIVDSDEVSTKILMEFNKMNLPGEVTFLPLNKLDVRDTAYPETNDAIPMISKLRYNPRFDKAFKHVFGKT LICRSMEVSTQLARAFTMDCITLEGDQVSHRGALTGGYYDTRKSRLELQKDVRKAEEELGELEAKLNENL RRNIERINNEIDQLMNQMQQIETQQRKFKASRDSILSEMKMLKEKRQQSEKTFMPKQRSLQSLEASLHAM ESTRESLKAELGTDLLSQLSLEDQKRVDALNDEIRQLQQENRQLLNERIKLEGIITRVETYLNENLRKRL DQVEQELNELRETEGGTVLTATTSELEAINKRVKDTMARSEDLDNSIDKTEAGIKELQKSMERWKNMEKE HMDAINHDTKELEKMTNRQGMLLKKKEECMKKIRELGSLPQEAFEKYQTLSLKQLFRKLEQCNTELKKYS HVNKKALDQFVNFSEQKEKLIKRQEELDRGYKSIMELMNVLELRKYEAIQLTFKQVSKNFSEVFQKLVPG GKATLVMKKGDVEGSQSQDEGEGSGESERGSGSQSSVPSVDQFTGVGIRVSFTGKQGEMREMQQLSGGQK SLVALALIFAIQKCDPAPFYLFDEIDQALDAQHRKAVSDMIMELAVHAQFITTTFRPELLESADKFYGVK FRNKVSHIDVITAEMAKDFVEDDTTHG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007790 |
| ORF Size | 3651 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007790.3, NP_031816.2 |
| RefSeq Size | 4851 bp |
| RefSeq ORF | 3654 bp |
| Locus ID | 13006 |
| UniProt ID | Q9CW03 |
| Gene Summary | Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223961 | Smc3 (untagged) - Mouse structural maintenace of chromosomes 3 (Smc3), (10ug) |
CNY 15,580.00 |
|
| MR211814 | Smc3 (Myc-DDK-tagged) - Mouse structural maintenace of chromosomes 3 (Smc3) |
CNY 13,560.00 |
|
| MR211814L3 | Lenti ORF clone of Smc3 (Myc-DDK-tagged) - Mouse structural maintenace of chromosomes 3 (Smc3) |
CNY 14,060.00 |
|
| MR211814L4 | Lenti ORF clone of Smc3 (mGFP-tagged) - Mouse structural maintenace of chromosomes 3 (Smc3) |
CNY 14,060.00 |
