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Pkd2 (NM_008861) Mouse Tagged ORF Clone
CAT＃: MG211337
- TrueORF®
Pkd2 (tGFP-tagged) - Mouse polycystic kidney disease 2 (Pkd2)
ORF Plasmid: DDK
"NM_008861" in other vectors (4)
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CNY 7,890.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C030034P18Rik; PC2; TRPP2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211337 representing NM_008861
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTAACTCCAGACGCGTGCAGCCGCAGCCGCCCGGGGACGCGGGACGCTCGCCCGCGCCGCGAGCGT CCGGACCCGGGCGCCTGGTGGCGGGAGGCGCCGGCCTAGCTGTCCCCGGCGGCCTCGGGGAGCAGCGGGG CCTGGAGATCGAGATGGAGCGCATCCGGCAGGCGGCCGCTCGGGATCCCCCGGCCGGAGCCTCGGCCTCG CCGTCTCCTCCGCTTTCGTCCTGCTCCAGGCAAGCGTGGAGCCGCGACAACCCGGGCTTTGAGGCAGAGG AGGATGACGACGACGACGAGGTGGAAGGAGAAGAAGGAGGGATGGTGGTAGAGATGGATGTGGAGTGGCG CCCGGGCAGTCGGAGGTCGGCCTCCTCCTCGGCCGTGAGCTCGGTGGGCGCCCGCGGCCGAGGGCTCGGG AGCTACCGCGGCGCGGCTCACCTGAGCGGGAGGCGGCGCCGGCTAGAGGACCAGGGCGCGCAGTGTCCCA GCCCCGCGGGCGGCGGGGACCCGCTGCATCGCCACCTCCCGCTGGAGGGCCAGCCACCCCGAGTGGCCTG GGCAGAGAGGCTGGTGCGAGGGCTGCGAGGTCTCTGGGGAACAAGACTCATGGAAGAGAGCAACGCCAAC CGAGAGAAGTACCTGAAAAGTGTGTTACGGGAGCTGGTCACTTACCTCTTTTTCCTCGTAGTCTTGTGCA TCTTGACCTACGGCATGATGAGCTCCAATGTGTACTACTACACTCGGACACTGTCACAGCTATTCATAGA CACCCCAGTGTCGAAAACAGAGAAAACCAACTTTAAAACTCTTTCTTCCATGGAGGACTTCTGGAAGTTC ACCGAAGGCTCCTTCCTGGATGGGCTGTACTGGAAGGCACAGACCAGCAACCACACGCAAGCTGACAACC GAAGCTTTATCTTCTATGAGAACCTGCTGCTAGGAGTGCCGCGTCTACGCCAACTCCGAGTCAGAAACGG ATCCTGCTCCATCCCTCAGGACCTGCGAGATGAAATTAAAGAGTGCTATGACGTCTACTCCGTCAGCAGT GAGGACAGAGCTCCATTTGGACCGCGGAATGGAACTGCGTGGATGTACACAAGTGAGAAGGAGCTGAATG GGAGCAGTCACTGGGGGATCATTGCGTCGTACAGTGGAGCGGGTTACTACCTGGATCTGTCCAGAACCAG GGAGGAGACAGCAGCCCAGCTTGCTGGCCTCAGGAGGAACTTCTGGCTGGACCGGGGCACGCGGGCAGCT TTTATAGACTTCTCGGTGTATAACGCAAACATTAACCTGTTCTGTGTGGTCAGGTTATTGGCGGAGTTCC CAGCAACGGGTGGCGTGGTACCCTCTTGGCAGTTTCAGCCTGTAAAACTGATCCGCTATGTCACAGCCTT TGATTTCTTCCTGGCAGCCTGTGAGATCATCTTTTGTTTCTTTATCATTTACTATGTGGTGGAAGAGATA TTGGAAATTCGGATTCACAGACTGAGCTATTTCAGGAGTTTCTGGAATTGTCTGGATGTTGTGATTGTCG TGTTATCTGTAGTAGCTATGGTGATTAACATTTACCGAATGTCAAATGCAGAGGGGCTGCTACAGTTTCT TGAAGATCAAAATTCTTTCCCCAACTTTGAGCATGTGGCATACTGGCAAATACAGTTCAACAATATAAGT GCTGTCATGGTATTTTTGGTCTGGATTAAGCTCTTCAAATTCATCAATTTTAATAGGACCATGAGCCAGC TCTCCACAACCATGTCTCGATGTGCCAAAGACCTCTTCGGCTTCACCATAATGTTCTCCATCATCTTCTT GGCATACGCACAGCTGGCATACCTTGTCTTCGGCACCCAGGTCGATGACTTCAGCACTTTCCAAGAATGT ATCTTCACCCAGTTCCGCATCATTTTGGGTGATATCAACTTCGCAGAGATCGAGGAAGCTAACCGAGTTT TGGGGCCACTTTATTTTACTACATTTGTGTTCTTTATGTTCTTCATTCTTTTGAATATGTTCCTGGCGAT CATCAATGATTCGTACTCTGAAGTGAAATCCGATCTGGCCCAGCAGAAAGCAGAAATGGAACTCTCAGAC CTTATCAGAAAGGGCTGCCAAAAAGCACTGGTCAAACTAAAACTGAAAAGAAACACTGTAGATGCCATCT CAGAGAGTCTCCGGCAAGGTGGTGGCAAACTGAACTTTGATGAGCTTCGGCAAGACCTGAAAGGGAAGGG CCATACAGATGCAGAGATTGAGGCCATATTCACTAAATATGACCAGGATGGCGACCAGGAACTGACCGAG CGTGAGCATCAACAGATGAGAGATGACTTGGAGAAAGAGAGGGAGGACCTAGACTTGGAACACAGCTCTT TACCACGTCCGATGAGCAGCAGAAGTTTCCCCAGAAGCCTGGATGACTCCGAGGAGGAGGATGACGAAGA CAGTGGCCATAGCTCCAGGAGGAGGGGAAGCATCTCCAGTGGGGTTTCCTATGAAGAGTTCCAAGTACTG GTGAGGCGCGTGGACCGCATGGAGCACTCCATTGGCAGCATCGTTTCCAAGATTGATGCCGTGATTGTCA AGCTGGAGATCATGGAGCGGGCCAAGCTGAAGAGACGAGAGGTGTTAGGACGGCTGCTGGATGGCGTGGC TGAGGATGCGCGACTGGGTCGGGACAGTGAGATCCACAGGGAGCAGATGGAGCGCCTGGTGCGGGAAGAG CTGGAGCGCTGGGAATCGGATGATGCAGCTTCGCAAACAGGTCATGGTGTAAGCACACAAGTGGGACTCG GTGGCCAGCCCCACCCCAGAAACCCGCGCCCTCCTTCCTCCCAGTCTGCAGAGGGCCTGGAAGGTGGAGG TGGAAATGGAAGTGCCAACGTCCATGCC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211337 representing NM_008861
Red=Cloning site Green=Tags(s) MVNSRRVQPQPPGDAGRSPAPRASGPGRLVAGGAGLAVPGGLGEQRGLEIEMERIRQAAARDPPAGASAS PSPPLSSCSRQAWSRDNPGFEAEEDDDDDEVEGEEGGMVVEMDVEWRPGSRRSASSSAVSSVGARGRGLG SYRGAAHLSGRRRRLEDQGAQCPSPAGGGDPLHRHLPLEGQPPRVAWAERLVRGLRGLWGTRLMEESNAN REKYLKSVLRELVTYLFFLVVLCILTYGMMSSNVYYYTRTLSQLFIDTPVSKTEKTNFKTLSSMEDFWKF TEGSFLDGLYWKAQTSNHTQADNRSFIFYENLLLGVPRLRQLRVRNGSCSIPQDLRDEIKECYDVYSVSS EDRAPFGPRNGTAWMYTSEKELNGSSHWGIIASYSGAGYYLDLSRTREETAAQLAGLRRNFWLDRGTRAA FIDFSVYNANINLFCVVRLLAEFPATGGVVPSWQFQPVKLIRYVTAFDFFLAACEIIFCFFIIYYVVEEI LEIRIHRLSYFRSFWNCLDVVIVVLSVVAMVINIYRMSNAEGLLQFLEDQNSFPNFEHVAYWQIQFNNIS AVMVFLVWIKLFKFINFNRTMSQLSTTMSRCAKDLFGFTIMFSIIFLAYAQLAYLVFGTQVDDFSTFQEC IFTQFRIILGDINFAEIEEANRVLGPLYFTTFVFFMFFILLNMFLAIINDSYSEVKSDLAQQKAEMELSD LIRKGCQKALVKLKLKRNTVDAISESLRQGGGKLNFDELRQDLKGKGHTDAEIEAIFTKYDQDGDQELTE REHQQMRDDLEKEREDLDLEHSSLPRPMSSRSFPRSLDDSEEEDDEDSGHSSRRRGSISSGVSYEEFQVL VRRVDRMEHSIGSIVSKIDAVIVKLEIMERAKLKRREVLGRLLDGVAEDARLGRDSEIHREQMERLVREE LERWESDDAASQTGHGVSTQVGLGGQPHPRNPRPPSSQSAEGLEGGGGNGSANVHA SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008861 |
| ORF Size | 2898 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008861.3, NP_032887.3 |
| RefSeq Size | 5221 bp |
| RefSeq ORF | 2901 bp |
| Locus ID | 18764 |
| UniProt ID | O35245 |
| Gene Summary | Component of a heteromeric calcium-permeable ion channel formed by PKD1 and PKD2 that is activated by interaction between PKD1 and a Wnt family member, such as WNT3A and WNT9B. Can also form a functional, homotetrameric ion channel (PubMed:27214281). Functions as a cation channel involved in fluid-flow mechanosensation by the primary cilium in renal epithelium (PubMed:12514735, PubMed:18695040, PubMed:27760766). Functions as outward-rectifying K(+) channel, but is also permeable to Ca(2+), and to a much lesser degree also to Na(+) (PubMed:27760766). May contribute to the release of Ca(2+) stores from the endoplasmic reticulum (By similarity). Together with TRPV4, forms mechano- and thermosensitive channels in cilium (PubMed:18695040). PKD1 and PKD2 may function through a common signaling pathway that is necessary to maintain the normal, differentiated state of renal tubule cells (PubMed:9568711, PubMed:10615132). Acts as a regulator of cilium length, together with PKD1. The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling. The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling (PubMed:20096584). Also involved in left-right axis specification via its role in sensing nodal flow; forms a complex with PKD1L1 in cilia to facilitate flow detection in left-right patterning (PubMed:21307093, PubMed:22983710). Detection of asymmetric nodal flow gives rise to a Ca(2+) signal that is required for normal, asymmetric expression of genes involved in the specification of body left-right laterality (PubMed:12062060, PubMed:21307093, PubMed:22983710).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222866 | Pkd2 (untagged) - Mouse polycystic kidney disease 2 (Pkd2), (10ug) |
CNY 12,350.00 |
|
| MR211337 | Pkd2 (Myc-DDK-tagged) - Mouse polycystic kidney disease 2 (Pkd2) |
CNY 7,072.00 |
|
| MR211337L3 | Lenti ORF clone of Pkd2 (Myc-DDK-tagged) - Mouse polycystic kidney disease 2 (Pkd2) |
CNY 9,030.00 |
|
| MR211337L4 | Lenti ORF clone of Pkd2 (mGFP-tagged) - Mouse polycystic kidney disease 2 (Pkd2) |
CNY 9,030.00 |
