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Vil1 (NM_009509) Mouse Tagged ORF Clone
CAT＃: MG210849
- TrueORF®
Vil1 (tGFP-tagged) - Mouse villin 1 (Vil1)
ORF Plasmid: DDK
"NM_009509" in other vectors (4)
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CNY 7,980.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Vil |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210849 representing NM_009509
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTAAACTGAATGCCCAAGTCAAAGGCTCTCTCAACATCACCACTCCCGGGATACAGATATGGAGGA TCGAGGCTATGCAGATGGTACCTGTTCCTTCCAGCACCTTTGGAAGCTTCTTCGATGGTGACTGCTATGT AGTCCTGGCTATCCACAAGACCAGCAGCACTCTCTCCTATGATATCCACTACTGGATTGGCCAGGACTCG TCCCAGGATGAGCAGGGGGCAGCTGCCATCTACACCACACAGATGGATGACTACCTGAAGGGCCGGGCTG TCCAGCACCGCGAGGTTCAAGGCAACGAGAGCGAGACTTTCCGGAGCTACTTCAAGCAAGGCCTTGTGAT CCGGAAAGGGGGAGTGGCTTCCGGCATGAAGCACGTAGAAACAAACTCCTGTGATGTCCAGCGACTGTTG CACGTCAAGGGCAAGAGGAATGTGCTGGCTGGAGAGGTGGAAATGTCCTGGAAGAGTTTCAACAGAGGGG ATGTCTTCCTGCTGGACCTTGGGAAGCTTATTATCCAGTGGAATGGGCCAGAGAGTAACCGCATGGAGAG ACTTCGGGGCATGGCCTTGGCCAAAGAGATCCGAGACCAGGAACGGGGTGGACGTACCTACGTAGGTGTG GTGGACGGGGAGAAGGAAGGGGACTCCCCACAGCTGATGGCAATTATGAACCACGTGCTGGGCCCACGCA AAGAACTGAAGGCTGCTATTTCTGACTCAGTGGTGGAGCCGGCCGCTAAGGCTGCACTCAAGCTGTACCA TGTGTCTGACTCCGAAGGAAAACTGGTGGTTAGAGAAGTTGCTACTCGGCCACTCACACAAGACCTGCTC AAGCATGAGGACTGTTACATCCTGGACCAGGGAGGCCTGAAGATCTTTGTGTGGAAGGGAAAAAATGCCA ACGCTCAGGAGAGGAGCGGAGCCATGAGCCAGGCCTTGAACTTCATCAAAGCCAAGCAGTACCCACCGAG CACGCAGGTTGAGGTGCAGAATGACGGGGCCGAGTCCCCCATCTTCCAACAACTCTTCCAGAAGTGGACA GTGCCCAACCGGACCTCAGGCCTCGGCAAAACCCACACTGTGGGCTCTGTGGCTAAGGTGGAACAGGTGA AGTTTGATGCTCTGACCATGCATGTACAACCTCAGGTGGCTGCCCAGCAGAAAATGGTGGATGACGGGAG TGGGGAAGTGCAGGTGTGGCGCATCGAGGACTTGGAGCTGGTGCCTGTGGAGTCCAAGTGGCTGGGCCAT TTCTACGGTGGTGACTGCTACCTGCTGCTCTACACCTACCTCATAGGGGAAAAGCAACACTACTTGTTAT ATATCTGGCAGGGCAGCCAGGCCAGCCAGGATGAAATTGCAGCCTCGGCGTATCAAGCCGTCCTGTTGGA CCAGAAGTACAATGACGAGCCAGTACAGATCCGGGTCACGATGGGCAAGGAGCCGCCTCACCTCATGTCT ATCTTCAAGGGCCGCATGGTGGTTTATCAGGGAGGCACCTCCCGAAAGAACAACTTGGAGCCTGTGCCCT CTACGAGGCTATTTCAGGTCCGAGGGACCAATGCTGATAACACCAAGGCTTTTGAGGTGACAGCCCGGGC CACGTCCCTCAACTCCAATGATGTCTTCATACTCAAGACTCCGTCCTGCTGCTACCTGTGGTGTGGGAAG GGCTGCAGTGGGGATGAGAGGGAGATGGCCAAGATGGTTGCTGATACCATCTCTCGGACGGAGAAACAAG TGGTAGTAGAGGGGCAGGAGCCAGCCAACTTCTGGATGGCTCTGGGCGGGAAGGCGCCCTACGCCAACAC CAAGAGGCTGCAGGAGGAAAACCAAGTCATCACTCCTCGGCTCTTCGAGTGCTCCAACCAGACCGGACGC TTTCTGGCCACAGAGATCTTTGACTTCAATCAGGATGACCTGGAGGAGGAGGATGTGTTCCTATTGGATG TCTGGGACCAGGTCTTCTTCTGGATAGGGAAACATGCCAATGAGGAAGAGAAGAAGGCTGCAGCTACAAC TGTACAAGAATACCTCAAGACCCACCCTGGAAACCGAGACCTTGAGACCCCTATCATCGTGGTGAAGCAG GGACACGAGCCCCCCACCTTCACAGGCTGGTTCCTGGCTTGGGATCCCTTCAAGTGGAGTAACACCAAAT CCTATGATGACCTTAAGGCAGAGCTGGGAAACTCTGGGGACTGGAGCCAGATTGCTGACGAGGTTATGAG CCCGAAAGTGGACGTTTTCACTGCCAATACCAGTCTGAGTTCTGGGCCCCTGCCCACCTTCCCCCTGGAG CAGCTGGTAAACAAGTCTGTAGAGGATCTCCCTGAGGGTGTGGACCCCAGCAGGAAGGAGGAGCACCTGT CCACCGAAGACTTCACTAGGGCCTTGGGCATGACTCCAGCTGCCTTCTCTGCCCTGCCTCGATGGAAGCA ACAAAACATCAAGAAAGAAAAAGGACTGTTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210849 representing NM_009509
Red=Cloning site Green=Tags(s) MTKLNAQVKGSLNITTPGIQIWRIEAMQMVPVPSSTFGSFFDGDCYVVLAIHKTSSTLSYDIHYWIGQDS SQDEQGAAAIYTTQMDDYLKGRAVQHREVQGNESETFRSYFKQGLVIRKGGVASGMKHVETNSCDVQRLL HVKGKRNVLAGEVEMSWKSFNRGDVFLLDLGKLIIQWNGPESNRMERLRGMALAKEIRDQERGGRTYVGV VDGEKEGDSPQLMAIMNHVLGPRKELKAAISDSVVEPAAKAALKLYHVSDSEGKLVVREVATRPLTQDLL KHEDCYILDQGGLKIFVWKGKNANAQERSGAMSQALNFIKAKQYPPSTQVEVQNDGAESPIFQQLFQKWT VPNRTSGLGKTHTVGSVAKVEQVKFDALTMHVQPQVAAQQKMVDDGSGEVQVWRIEDLELVPVESKWLGH FYGGDCYLLLYTYLIGEKQHYLLYIWQGSQASQDEIAASAYQAVLLDQKYNDEPVQIRVTMGKEPPHLMS IFKGRMVVYQGGTSRKNNLEPVPSTRLFQVRGTNADNTKAFEVTARATSLNSNDVFILKTPSCCYLWCGK GCSGDEREMAKMVADTISRTEKQVVVEGQEPANFWMALGGKAPYANTKRLQEENQVITPRLFECSNQTGR FLATEIFDFNQDDLEEEDVFLLDVWDQVFFWIGKHANEEEKKAAATTVQEYLKTHPGNRDLETPIIVVKQ GHEPPTFTGWFLAWDPFKWSNTKSYDDLKAELGNSGDWSQIADEVMSPKVDVFTANTSLSSGPLPTFPLE QLVNKSVEDLPEGVDPSRKEEHLSTEDFTRALGMTPAAFSALPRWKQQNIKKEKGLF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009509 |
| ORF Size | 2481 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009509.2 |
| RefSeq Size | 3060 bp |
| RefSeq ORF | 2484 bp |
| Locus ID | 22349 |
| UniProt ID | Q62468 |
| Gene Summary | Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201154 | Vil1 (untagged) - Mouse villin 1 (Vil1), (10ug) |
CNY 2,000.00 |
|
| MR210849 | Vil1 (Myc-DDK-tagged) - Mouse villin 1 (Vil1) |
CNY 6,064.00 |
|
| MR210849L3 | Lenti ORF clone of Vil1 (Myc-DDK-tagged) - Mouse villin 1 (Vil1) |
CNY 9,120.00 |
|
| MR210849L4 | Lenti ORF clone of Vil1 (mGFP-tagged) - Mouse villin 1 (Vil1) |
CNY 9,120.00 |
