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Hells (NM_008234) Mouse Tagged ORF Clone
CAT＃: MG210821
- TrueORF®
Hells (tGFP-tagged) - Mouse helicase, lymphoid specific (Hells)
ORF Plasmid: DDK
"NM_008234" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI323785; E130115I21Rik; LSH; Lysh; PASG; YFK8 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210821 representing NM_008234
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAACAAACGGAGCCTGCGGTGATTACTCCAGCCATGCTGGAGGAGGAGGAACAGCTCGAGGCTG CGGGACTTGAGAAAGAGCGGAAGATGTTAGAAGAGGCTCAAAAGTCTTGGGACAGAGAGTCCACTGAAAT TCGGTACCGCAGACTTCAGCATTTGCTTGAAAAAAGCAATATCTACTCAAAATTTTTGTTGACTAAAATG GAACAACAGCAATTGGAGGAACAGAAGAAGAAAGAGAAGTTGGAGAAAAAAAAACGGTCATTAAAACTTA CAGAGGGTAAAAGTTTGGTTGATGGGAATGGAGAGAAACCAGTCATGAAAAAGAAAAGAGGAAGAGAAGA TGAATCTTATAATATTTCAGAGGTCATGTCAAAAGAGGAAATTTTGTCCGTAGCTAAAAAACATAAGGAT AATGAGGATGAAAGCTCTTCCACTACGAGTCTTTGTGTAGAAGATATTCAGAAAAATAAGGATTCAAATA GTATGATTAAAGATAGATTGTCTCAAACTGTTAGGCAGAATTCTAAATTCTTTTTTGACCCAGTTCGGAA ATGTAACGGACAGCCTGTACCCTTTCAACAACCAAAGCATTTCACAGGAGGAGTAATGAGGTGGTACCAA GTAGAAGGCATGGAATGGCTTAGGATGCTTTGGGAAAATGGAATTAATGGCATTTTAGCAGATGAAATGG GTTTGGGAAAGACTGTTCAGTGCATTGCTACAATTGCATTGATGATTCAGAGGGGAGTACCTGGACCTTT TCTTGTTTGTGGCCCTTTGTCTACACTTCCTAACTGGATGGCTGAATTTAAAAGATTTACTCCAGAAATT CCTACTCTGCTGTATCATGGAACCCGGGAGGACCGTCGGAAATTAGTAAAGAATATCCACAAAAGACAAG GGACACTGCAGATTCATCCTGTGGTGGTCACATCATTCGAGATCGCTATGCGAGACCAGAATGCTTTACA GCATTGCTATTGGAAATACTTAATAGTAGATGAAGGACACAGGATTAAGAATATGAAGTGCCGGCTAATC AGGGAGTTAAAACGGTTCAATGCTGATAACAAACTTCTTTTGACTGGTACTCCCTTGCAAAACAATTTAT CAGAACTTTGGTCATTGCTAAACTTTTTGTTGCCAGATGTATTTGATGACTTGAAAAGCTTTGAGTCTTG GTTTGACATCACTAGTCTTTCTGAAACTGCTGAAGATATTATAGCTAAAGAGAGAGAACAGAATGTTTTA CATATGCTGCACCAGATTTTAACGCCTTTCCTACTGAGAAGACTGAAGTCTGATGTTGCTCTTGAAGTCC CCCCTAAGCGAGAAGTAGTTGTTTATGCACCACTTTGCAATAAACAAGAGATCTTCTATACAGCTATTGT GAACCGCACAATTGCAAACATGTTTGGATCCTGTGAGAAGGAAACAGTTGAGCTAAGTCCTACTGGAAGA CCAAAACGACGAAGTAGAAGGTCAATAAATTACAGCGAACTAGATCAGTTCCCTAGTGAATTAGAAAAAC TAATAAGTCAAATACAGCCAGAAGTAAACAGAGAGAGGACTGTTGTGGAAGGTAATATCCCTATAGAATC TGAAGTTAATCTGAAGCTGCGGAATATAATGATGCTACTTCGAAAGTGCTGTAATCACCCATATATGATT GAATATCCTATTGACCCTGTCACACAAGAATTTAAGATTGATGAAGAGTTGGTAACAAATTCTGGGAAGT TCTTAATTTTGGATCGAATGCTGCCCGAACTTAAAAAAAGAGGTCACAAGGTTCTGGTGTTTTCACAAAT GACAAGCATGTTGGACATCTTGATGGATTATTGCCATCCTAGAAACTTCATCTTTAGCAGACTTGATGGG TCCATGTCTTATTCGGAAAGAGAAAAAAATATATACAGTTTCAACACGGATCCAGATGTTTTTCTTTTCT TAGTGAGTACACGAGCAGGTGGTTTAGGCATAAACTTGACTGCAGCAGATACAGTGATCATTTATGACAG TGATTGGAATCCTCAGTCTGATCTCCAGGCTCAAGATAGATGTCATAGAATTGGTCAGACAAAGCCAGTT GTTGTGTATCGTCTTGTAACAGCAAATACTATTGACCAGAAAATTGTGGAAAGAGCAGCTGCTAAAAGAA AGTTGGAGAAGTTGATAATCCATAAAAATCATTTCAAAGGTGGTCAGTCTGGGTTAAGTCAATCTAAGAA TTTCTTAGATGCAAAGGAATTAATGGAGTTATTAAAATCTAGAGATTATGAAAGGGAAGTAAAAGGATCC AGAGAGAAGGTCATTAGTGACGAAGATCTAGAATTACTGTTGGATCGAAGTGATCTCATTGATCAAATGA AGGCTTCAAGACCAATTAAAGGGAAGACAGGGATATTCAAAATACTAGAAAATTCTGAAGATTCCAGTGC TGAATGTTTATTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210821 representing NM_008234
Red=Cloning site Green=Tags(s) MAEQTEPAVITPAMLEEEEQLEAAGLEKERKMLEEAQKSWDRESTEIRYRRLQHLLEKSNIYSKFLLTKM EQQQLEEQKKKEKLEKKKRSLKLTEGKSLVDGNGEKPVMKKKRGREDESYNISEVMSKEEILSVAKKHKD NEDESSSTTSLCVEDIQKNKDSNSMIKDRLSQTVRQNSKFFFDPVRKCNGQPVPFQQPKHFTGGVMRWYQ VEGMEWLRMLWENGINGILADEMGLGKTVQCIATIALMIQRGVPGPFLVCGPLSTLPNWMAEFKRFTPEI PTLLYHGTREDRRKLVKNIHKRQGTLQIHPVVVTSFEIAMRDQNALQHCYWKYLIVDEGHRIKNMKCRLI RELKRFNADNKLLLTGTPLQNNLSELWSLLNFLLPDVFDDLKSFESWFDITSLSETAEDIIAKEREQNVL HMLHQILTPFLLRRLKSDVALEVPPKREVVVYAPLCNKQEIFYTAIVNRTIANMFGSCEKETVELSPTGR PKRRSRRSINYSELDQFPSELEKLISQIQPEVNRERTVVEGNIPIESEVNLKLRNIMMLLRKCCNHPYMI EYPIDPVTQEFKIDEELVTNSGKFLILDRMLPELKKRGHKVLVFSQMTSMLDILMDYCHPRNFIFSRLDG SMSYSEREKNIYSFNTDPDVFLFLVSTRAGGLGINLTAADTVIIYDSDWNPQSDLQAQDRCHRIGQTKPV VVYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLSQSKNFLDAKELMELLKSRDYEREVKGS REKVISDEDLELLLDRSDLIDQMKASRPIKGKTGIFKILENSEDSSAECLF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008234 |
| ORF Size | 2463 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008234.3, NP_032260.2 |
| RefSeq Size | 3039 bp |
| RefSeq ORF | 2466 bp |
| Locus ID | 15201 |
| UniProt ID | Q60848 |
| Gene Summary | Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221853 | Hells (untagged) - Mouse helicase, lymphoid specific (Hells), (10ug) |
CNY 10,550.00 |
|
| MR210821 | Hells (Myc-DDK-tagged) - Mouse helicase, lymphoid specific (Hells) |
CNY 5,800.00 |
|
| MR210821L3 | Lenti ORF clone of Hells (Myc-DDK-tagged) - Mouse helicase, lymphoid specific (Hells) |
CNY 7,700.00 |
|
| MR210821L4 | Lenti ORF clone of Hells (mGFP-tagged) - Mouse helicase, lymphoid specific (Hells) |
CNY 7,700.00 |
