Acap1 (NM_153788) Mouse Tagged ORF Clone

CAT＃: MG210394

TrueORF^®

Acap1 (tGFP-tagged) - Mouse centaurin, beta 1 (Centb1)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_153788" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,370.00

货期*

4周

规格

Product images

经常一起买 (4)

PS100010

pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug

CNY 6,080.00

TA150041

Mouse monoclonal turboGFP antibody, clone OTI2H8

CNY 1,000.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Centb1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210394 representing NM_153788 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGTCAAGCTAGATTTTGAAGAATGTCTCAAGGACTCACCCCGCTTCCGAGCCTCGATTGAACTGG TGGAAACTGAAGTGTCAGAATTGGAGACCCGCCTGGAAAAGCTGCTAAAGCTGGGTTCCTGCCTCCTGGA GAGCGGACAGCATTACCTTGCTGCCGGTCGTGCCTTCGTTGTTGGCATCTGTGACCTGGCTCGCCTGGGT CCACCAGAGCCCATGATGGCGGAGTGTCTGGAAAAATTCACCGTGAGTCTGAACCACAAACTGGACAGCC ATGCTGAGCTTCTTGATGCTACTCAACATACACTGCAGCAGCAAATCCAGACTCTGGTCAAGGAAGGTCT CCGGGGCTTCCGAGAGGCTCGCAGGGATTTCTGGCGAGGGGCTGAGAACCTGGAGGCTGCTCTTACCCAC AATGCAGAGGTCCCCAGGCGCCGTGTCCAAGAAGCAGAGGAGGCTGGAACTGCTTTGAGGACCGCTCGAG CTGGGTACCGGAGTCGGGCATTAGATTATGCCCTGCAGGTCAATGTGATTGAGGATAAGAGGAAGTTCGA CATCATGGAATTCGTACTGCGCTTGGTTGAGGCCCAGGCCACTTATTTCCAGCAGGGCCATGAAGAGCTA AACAGGCTGGCTCAGTATCGGAAGGAATTGGGTACCCAGTTGCACAACCTGGTCTTGAACTCAGCTCGAC AGAAGAGAGACATGGAACAGAGGCATGTGTTACTGAAGCAGAAGGAGCTGGGTGGCGAGGAGCCAGAGCC AAGCCTAAAGGAGGGGCCTAGTGGCTTGGTAATGGAAGGACATCTTTTCAAACGTGCCAGCAATGCATTT AAGACCTGGAGCAGACGCTGGTTCACCATTCAGAACAACCAGCTGGTTTACCAGAAGAAATATAAGGACC CTGTAACCGTGGTGGTAGACGACCTTCGTCTCTGTACAGTGAAGCTCTGTCCAGACTCAGAAAGGCGTTT CTGTTTCGAAGTAGTGTCCACCAGCAAGTCCTGTTTCCTCCAGGCTGACTCTGAGCGTCTCCTGCAGCTG TGGGTCAGTGCTGTCCAGAGCAGCATTGCCTCAGCCTTCAGCCAGGCTCACCTGGAGAACAGCCCTCGGG GGCCAGGCCAGGTCTCAGGATACCATGCCCCGGGCTCTGCTGCAACTCTGGCCTGTGGCGGGGCAGCCAG AGGAAGGGAGTCTGGAGGAGTTGGTCAGGTAGCGGCCCAGGTACAAAGCGTGGATGGAAATGCGCAATGC TGCGACTGCAGGGAGCCAGCCCCAGAGTGGGCCAGCATCAACCTTGGCGTCACTCTCTGCATTCAGTGTT CTGGCATCCACAGGAGCCTCGGAGTTCATTTCTCCAAAGTCCGCTCTCTGACCCTTGACTCCTGGGAACC AGAACTGGTGAAGCTTATGTGCGAGCTGGGGAATGTCATCATCAACCAGATCTATGAGGCCCGGGTGGAG GCCATGGCGGTGAAGAAGCCAGGGCCCAGCTGCTCCCGGCAGGAAAAGGAAGCCTGGATTCATGCCAAGT ATGTGGAGAAAAAGTTCCTGACCAAACTTCCTGAAATCCGTGGGCGAAGAGGTGGCAGGGGACCCCCAAG AGGACATCCTCCTGTGCCCCCAAAGCCTCCCATCAGGCCACACTCGGGCATTGTCAGATCCAAATCAGAG TGCCCGTCTGATGACATGGGAAGCCTGCACCCCGGGGCCCTGCTGTTTCAAGCTGCTGGACATCCCCCAT CTCTTCCTACCATGGCTGATGCCCTGGCCCATGGAGCTGATGTCAACTGGGTCAATGTGGGCCAGGGGAA TGCCACACCTCTGATCCGGGCCACAGCTGCTAACTCTCTTCTGGCCTGCGAGTTTCTCCTCCAAAATGGG GCGAATGTGAACCAAGCAGACAGTGCCGGCCGGGGTCCTCTCCACCATGCCACCATTCTTGGTCACACAG GGCTCGCTTGCCTGTTCCTGAAACGGGGTGCTGATCTGGGGGCTCGAGACACAGAAGGCAGGGACCCTCT GACTATCGCAATGGAAACCACTAATGCTGACATCGTAACCCTGCTACGATTGGCAAAGATGAGGGAGGCT GAAGCGGCCCAGGGCCAGGCAGGAGACGAGACGTATCTCGACATATTCCGAGACTTCTCCCTCATGGCGT CGGACGACCCAGAGAAACTGAGCCGTCGCAGTCACGACCTCCATACTCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210394 representing NM_153788 Red=Cloning site Green=Tags(s) MTVKLDFEECLKDSPRFRASIELVETEVSELETRLEKLLKLGSCLLESGQHYLAAGRAFVVGICDLARLG PPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAENLEAALTH NAEVPRRRVQEAEEAGTALRTARAGYRSRALDYALQVNVIEDKRKFDIMEFVLRLVEAQATYFQQGHEEL NRLAQYRKELGTQLHNLVLNSARQKRDMEQRHVLLKQKELGGEEPEPSLKEGPSGLVMEGHLFKRASNAF KTWSRRWFTIQNNQLVYQKKYKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSTSKSCFLQADSERLLQL WVSAVQSSIASAFSQAHLENSPRGPGQVSGYHAPGSAATLACGGAARGRESGGVGQVAAQVQSVDGNAQC CDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVIINQIYEARVE AMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIRGRRGGRGPPRGHPPVPPKPPIRPHSGIVRSKSE CPSDDMGSLHPGALLFQAAGHPPSLPTMADALAHGADVNWVNVGQGNATPLIRATAANSLLACEFLLQNG ANVNQADSAGRGPLHHATILGHTGLACLFLKRGADLGARDTEGRDPLTIAMETTNADIVTLLRLAKMREA EAAQGQAGDETYLDIFRDFSLMASDDPEKLSRRSHDLHTL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_153788
ORF Size	2220 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_153788.2
RefSeq Size	2482 bp
RefSeq ORF	2223 bp
Locus ID	216859
UniProt ID	Q8K2H4
Gene Summary	GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MC202886	Acap1 (untagged) - Mouse ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (Acap1), (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,000.00
MR210394	Acap1 (Myc-DDK-tagged) - Mouse ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (Acap1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,432.00 CNY 5,800.00
MR210394L3	Lenti ORF clone of Acap1 (Myc-DDK-tagged) - Mouse ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (Acap1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00
MR210394L4	Lenti ORF clone of Acap1 (mGFP-tagged) - Mouse ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (Acap1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00

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