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Capn10 (NM_011796) Mouse Tagged ORF Clone
CAT＃: MG209889
- TrueORF®
Capn10 (tGFP-tagged) - Mouse calpain 10 (Capn10)
ORF Plasmid: DDK
"NM_011796" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW049679; Capn8; mKIAA1845 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209889 representing NM_011796
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGCGGTCCGGGCCGAGACGCCGGCGCGGGAGCTCTTCCGGGACGCGGCATTCCCCGCCTCGGACT CCTCGCTCTTTTACAACTTGTCCACGCCTCTGGCCCAGTTTCGGGAGGACATCACTTGGAGACGACCCCA GGAAATCTGTGCCACACCTCAGCTGTTTCCAGATAACCCATGGGAGGGACAGGTGAAGCAAGGGCTGCTG GGAGATTGCTGGTTCCTGTGTGCCTGTGCCGCCCTTCAGAAGAGTCAACACCTCCTGGACCAGGTCTTCC CTCCAGGACAGCCAGGCTGGTCTGACCAGAAATACCAAGGCTTCTTCACCTGTCGGATTTGGCAGTTTGG ACACTGGGAGGAAGTGACCATAGATGATCGTCTGCCTTGTCTTGCCGGGAGACTCTGCTTTTCCCGGTGC CAGAGAGAGGATGTGTTCTGGCTTCCCTTACTGGAAAAGGCCTATGCTAAGGTCCATGGATCGTATGAGC ACCTGTGGGCAGGGCAAGTGGCAGATGCCTTAGTGGATCTCACTGGAAGCCTGGCAGAAAGGTGGAGCTT GAAGGATGTAACGAAAGCCAGCGGCCAGCAGGACAGACCCAGTGGTGGGGAGCACAGAACTTGTCGGCAG CTACTCCACCTGAAGGACCGGTGTCTAATCAGCTGCTCTGTGCTTAGCCCCAGAGCAGGTGCCAGGGAAC TCGGAGAGTTCCATGCCTTCATCATCTCAGATCTGCAGGAGCTCAGGAGTCAGACTGGCCAGGGTATCCT CCTGCTGCGGATTCACAACCCCTGGGGCCGGCGTTGTTGGCAGGGCCTCTGGAGAGAAGGAGGTGAAGGG TGGAACCAGGTAGAGCCAGCTAAGGAGTCTGAGCTGCTGGCCCAACTCCAGGAAGGAGAGTTCTGGGTCG AGGAAGAGGAGTTCCTCAGGGAGTTTGATGAGGTCACCATCGGCTACCCAGTCACAGAGGCCGGCCACCT ACAGAGTCTCCACACAGAGAGGGTGCTGTGCCATACGCGGACACTGCCTGGTGCCTGGGTGACAGGGCAG TCAGCAGGAGGCTGCCGGAACAACAGTTGCTTTCCCTGCAACCCCAAGTTCTGGTTACGGCTCTTGGAAC CCAGCGAGGTGTGTGTGGCTGTTCTTCAGAGACCCCGGAGGCGCTTAGTGGGCCAGACTCGGGCACTGGC GGGTGCCAGTCCTGCACCGGTGAACCTCCCAGGCAAAGACTACCAGGCTGTGGGCCTGCACATCTGGAAG GTAGAGAAACGGAAGATCAGCCTGCCCAGAGTCCTGTCTGCACCCCCTGTGGCTGGCACTGCATGCCATG CGTATGATCGTGAGATCCACTTGCGTTGTGAGCTCTCACCAGGCTACTACCTGGCCGTCCCTAGCACCTT TTTGAAGGATGTGCCAGGGCAGTTCCTGCTCAGAGTCTTCTCCACTGGGAAAATCTCCCTCAGTGCCGTC AGGCTGGCCACCAAGGGTGCATCGCCTGGAACAGCCCTGCCTGCAGGCGAGTGGGAGACTGTGCAGTTGC AGGGCTGCTGGAGAGCTGGCCAGACAGCTGGGGGCAGCAGGAACTTTGCCTCTTACCCCTGCAATCCCTG CCTCCCTTTCTCTGTTCCTGAGGGTGCTGGCCCCCGCTACATCCGTATCACCCTACAGCAACACTGCCGG CTCAGTGACAGCCAGCTGCACCCCATTGGTTTCCATGTCTTTCAGGTTCCAGCAGACGGTGAGAACCAGG ACGCGTGTTCCCTGCTGCTCCAGGAGCCACTGCTAAGCTGTGTACCACATCGCTACGCCCAGGAAGTGAG CCGCCTCTGCCTCCTTTCTGTGGGGAACTACAGGATTGTTCCCTCCACCTACCTGCCAGATACAGAGGGT ACCTTCACGGTAACCATAGCAACCAGAATCGATAGGCAGTCCATCCACAGCCAGGAGATGCTGGGCCAGC TGCTCCAGGAGGTCTCCTTTATGGCAGTGATGAAAGCC ACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209889 representing NM_011796
Red=Cloning site Green=Tags(s) MRAVRAETPARELFRDAAFPASDSSLFYNLSTPLAQFREDITWRRPQEICATPQLFPDNPWEGQVKQGLL GDCWFLCACAALQKSQHLLDQVFPPGQPGWSDQKYQGFFTCRIWQFGHWEEVTIDDRLPCLAGRLCFSRC QREDVFWLPLLEKAYAKVHGSYEHLWAGQVADALVDLTGSLAERWSLKDVTKASGQQDRPSGGEHRTCRQ LLHLKDRCLISCSVLSPRAGARELGEFHAFIISDLQELRSQTGQGILLLRIHNPWGRRCWQGLWREGGEG WNQVEPAKESELLAQLQEGEFWVEEEEFLREFDEVTIGYPVTEAGHLQSLHTERVLCHTRTLPGAWVTGQ SAGGCRNNSCFPCNPKFWLRLLEPSEVCVAVLQRPRRRLVGQTRALAGASPAPVNLPGKDYQAVGLHIWK VEKRKISLPRVLSAPPVAGTACHAYDREIHLRCELSPGYYLAVPSTFLKDVPGQFLLRVFSTGKISLSAV RLATKGASPGTALPAGEWETVQLQGCWRAGQTAGGSRNFASYPCNPCLPFSVPEGAGPRYIRITLQQHCR LSDSQLHPIGFHVFQVPADGENQDACSLLLQEPLLSCVPHRYAQEVSRLCLLSVGNYRIVPSTYLPDTEG TFTVTIATRIDRQSIHSQEMLGQLLQEVSFMAVMKA TRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-NotI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011796 |
| ORF Size | 1998 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011796.2, NP_035926.2 |
| RefSeq Size | 2877 bp |
| RefSeq ORF | 2001 bp |
| Locus ID | 23830 |
| UniProt ID | Q9ESK3 |
| Gene Summary | Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. May play a role in insulin-stimulated glucose uptake (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205652 | Capn10 (untagged) - Mouse calpain 10 (Capn10), (10ug) |
CNY 2,000.00 |
|
| MR209889 | Capn10 (Myc-DDK-tagged) - Mouse calpain 10 (Capn10) |
CNY 4,888.00 |
|
| MR209889L3 | Lenti ORF clone of Capn10 (Myc-DDK-tagged) - Mouse calpain 10 (Capn10) |
CNY 7,700.00 |
|
| MR209889L4 | Lenti ORF clone of Capn10 (mGFP-tagged) - Mouse calpain 10 (Capn10) |
CNY 7,700.00 |
