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Prmt5 (NM_013768) Mouse Tagged ORF Clone
CAT＃: MG209659
- TrueORF®
Prmt5 (tGFP-tagged) - Mouse protein arginine N-methyltransferase 5 (Prmt5)
ORF Plasmid: DDK
"NM_013768" in other vectors (4)
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CNY 6,180.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Jbp; Jbp1; Skb; Skb1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209659 representing NM_013768
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGATGGCAGTCGGAGGTGCTGGTGGCAGCCGCGTGTCCAGCGGGAGGGACCTGAATTGCGTCC CCGAAATAGCTGACACACTGGGTGCTGTGGCCAAGCAGGGGTTTGATTTCCTCTGCATGCCTGTCTTCCA CCCGCGTTTCAAGAGGGAGTTCATTCAGGAACCTGCTAAGAATCGGCCTGGCCCCCAGACACGATCAGAC CTACTGCTGTCAGGAAGGGACTGGAATACGCTAATTGTGGGAAAGCTTTCTCCATGGATTCATCCAGACT CAAAAGTGGAGAAGATCCGAAGGAACTCTGAAGCGGCTATGTTACAGGAGCTGAATTTTGGAGCATATCT GGGTCTTCCAGCTTTCCTATTGCCCCTTAATCAGGAAGATAACACGAATCTGGCCAGAGTTCTGACCAAC CACATCCACACTGGCCACCACTCTTCCATGTTCTGGATGAGGGTACCCTTGGTGGCACCAGAGGACCTGA GAGATGATGTAATTGCGAATGCCCCGACTACACACACAGAGGAGTACAGTGGAGAAGAGAAGACATGGAT GTGGTGGCATAACTTTCGGACTCTGTGTGACTATAGCAAGAGAATTGCAGTAGCTCTTGAAATTGGAGCT GACCTCCCGTCTAATCACGTCATTGACCGCTGGCTTGGAGAGCCCATCAAAGCAGCCATTCTCCCCACCA GCATTTTCCTAACCAACAAGAAAGGATTTCCTGTTCTTTCTAAGGTGCAGCAGAGGCTGATCTTCCGGCT CCTCAAGTTGGAAGTGCAGTTTATCATCACGGGAACCAACCACCACTCAGAGAAGGAGTTCTGTTCCTAC CTCCAGTACTTGGAATACTTAAGCCAAAATCGCCCTCCACCCAATGCCTATGAGCTCTTTGCCAAAGGCT ATGAAGACTATCTGCAGTCCCCACTCCAGCCTCTGATGGACAATCTGGAATCTCAGACATATGAAGTGTT TGAAAAGGACCCCATCAAATACTCTCAATATCAGCAGGCTATTTATAAATGTTTGCTAGACCGAGTACCA GAAGAAGAAAAGGAGACCAATGTCCAGGTACTTATGGTGCTGGGTGCAGGCCGGGGTCCTCTTGTGAATG CGTCTCTTCGGGCAGCCAAACAGGCCGAGCGGCGGATCAGGCTGTATGCTGTGGAGAAGAACCCCAATGC TGTGGTGACGCTAGAGAACTGGCAGTTTGAAGAATGGGGGAGCCAGGTGACAGTTGTCTCATCAGACATG CGGGAATGGGTGGCTCCGGAGAAAGCTGACATTATTGTCAGTGAGCTTCTGGGTTCCTTTGCCGACAACG AGCTGTCACCTGAGTGTCTGGATGGAGCACAGCACTTCCTGAAAGATGATGGCGTGAGCATCCCTGGAGA ATACACCTCCTTCCTGGCTCCCATTTCTTCCTCTAAGCTGTACAACGAGGTCCGTGCCTGTCGGGAAAAG GACCGCGATCCTGAGGCACAGTTTGAGATGCCTTATGTGGTTCGGTTGCACAACTTCCACCAGCTCTCTG CTCCTAAGCCCTGCTTTACCTTCAGCCATCCCAACCGAGATCCTATGATTGACAACAACCGCTACTGTAC CCTGGAGTTTCCTGTGGAGGTGAACACGGTGCTTCATGGCTTCGCAGGCTACTTTGAGACTGTGCTTTAC CGGGACATCACTCTGAGTATCCGCCCAGAGACTCACTCTCCTGGAATGTTCTCATGGTTCCCCATCTTCT TCCCCATTAAGCAGCCCATCACGGTGCACGAAGGCCAGAACATCTGTGTGCGTTTCTGGCGATGCAGCAA TTCCAAGAAAGTGTGGTACGAGTGGGCGGTGACGGCCCCCGTCTGTTCTTCTATTCACAACCCTACCGGC CGCTCCTATACCATTGGCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209659 representing NM_013768
Red=Cloning site Green=Tags(s) MAAMAVGGAGGSRVSSGRDLNCVPEIADTLGAVAKQGFDFLCMPVFHPRFKREFIQEPAKNRPGPQTRSD LLLSGRDWNTLIVGKLSPWIHPDSKVEKIRRNSEAAMLQELNFGAYLGLPAFLLPLNQEDNTNLARVLTN HIHTGHHSSMFWMRVPLVAPEDLRDDVIANAPTTHTEEYSGEEKTWMWWHNFRTLCDYSKRIAVALEIGA DLPSNHVIDRWLGEPIKAAILPTSIFLTNKKGFPVLSKVQQRLIFRLLKLEVQFIITGTNHHSEKEFCSY LQYLEYLSQNRPPPNAYELFAKGYEDYLQSPLQPLMDNLESQTYEVFEKDPIKYSQYQQAIYKCLLDRVP EEEKETNVQVLMVLGAGRGPLVNASLRAAKQAERRIRLYAVEKNPNAVVTLENWQFEEWGSQVTVVSSDM REWVAPEKADIIVSELLGSFADNELSPECLDGAQHFLKDDGVSIPGEYTSFLAPISSSKLYNEVRACREK DRDPEAQFEMPYVVRLHNFHQLSAPKPCFTFSHPNRDPMIDNNRYCTLEFPVEVNTVLHGFAGYFETVLY RDITLSIRPETHSPGMFSWFPIFFPIKQPITVHEGQNICVRFWRCSNSKKVWYEWAVTAPVCSSIHNPTG RSYTIGL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013768 |
| ORF Size | 1911 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013768.2 |
| RefSeq Size | 2328 bp |
| RefSeq ORF | 1914 bp |
| Locus ID | 27374 |
| UniProt ID | Q8CIG8 |
| Gene Summary | This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation and the assembly of small nuclear ribonucleoproteins. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205387 | Prmt5 (untagged) - Mouse protein arginine N-methyltransferase 5 (Prmt5), (10ug) |
CNY 2,000.00 |
|
| MR209659 | Prmt5 (Myc-DDK-tagged) - Mouse protein arginine N-methyltransferase 5 (Prmt5) |
CNY 4,752.00 |
|
| MR209659L3 | Lenti ORF clone of Prmt5 (Myc-DDK-tagged) - Mouse protein arginine N-methyltransferase 5 (Prmt5) |
CNY 7,510.00 |
|
| MR209659L4 | Lenti ORF clone of Prmt5 (mGFP-tagged) - Mouse protein arginine N-methyltransferase 5 (Prmt5) |
CNY 7,510.00 |
