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Cdt1 (NM_026014) Mouse Tagged ORF Clone
CAT＃: MG208824
- TrueORF®
Cdt1 (tGFP-tagged) - Mouse chromatin licensing and DNA replication factor 1 (Cdt1)
ORF Plasmid: DDK
"NM_026014" in other vectors (4)
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CNY 6,180.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2610318F11Rik; AW545653; C76791; Ris2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208824 representing NM_026014
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAAAGTCGTGTTACCGATTTCTATGCGTGCCGTCGCCCTGGCCTTACGACTCCGCGGGCCAAGT CGATCTGTCTCACCCCGAGCCCTGGTGGCCTCGTGGCTCCTGCGTTCACCCGGAGCAGCAGCCGCAAGCG CGCCCGGCCCCCAGCCGAACCCGGGAGTGACCAGCCCGCGCCGCTCGCGCGCCGGAGGTTACGGCTGCCT GGATTGGACTCCTGCCCCAGTTCTCTGCCTGAGCCCAGTTCCCCAGCTGAGCCCAGCCCTCCAGCTGACC CTAGCCCTCCAGCTGACCCTGGCTCCCCCGTTTGCCCATCCCCCGTCAAGCGAACAAAGAGTACAACTGT TTATGTTGGTCAACAGCCGGGCAAGATCCCCTCAGAGGACTCCGTCTCTGAGCTCCAGTCCTGCCTGAGG CGGGCACGGAAGTTGGGAGCCCAGGCACGGGCCCTGAGAGCCCGAGTCCAAGAGAATGCTGTGGAGCCTA GTACCCCAGATGCCAAGGTGCCCACAGAGCAACCATGTGTCGAGAAAGCTCCTGCCTACCAGCGCTTCCA TGCTCTGGCTCAGCCTGGTCTCCCAGGCCTTGTCCTACCCTACAAGTATCAGGTGCTAGTTGAGATGTTC CGCAGCATGGACACCATTGTGAGCATGCTCCACAATCGCTCTGAGACTGTGACCTTTGCCAAAGTCAAGC AAGGTGTTCAGGAGATGATGCGCAAGCGCTTTGAAGAGCGCAATGTGGGCCAGATCAAAACCGTGTATCC CACGTCGTATCGCTTCCGCCAGGAGTGCAATGTCCCCACCTTCAAGGACAGCATCAAGAGATCTGATTAC CAGCTCACCATCGAGCCCTTGCTGGGCCAGGAGGCTGGCGGTGCCACCCAGCTCACAGCCACGTGCCTCC TGCAGCGCCGGCAAGTCTTCCGGCAGAACCTGGTGGAACGTGTCAAGGAACAGCACAAGGTCTTCCTGGC TTCACTGAACCCCCCCATGGCGGTGCCGGACGACCAGCTGACCCGCTGGCATCCGCGCTTCAATGTGGAC GAGGTGCCTGACATTGAGCCAGCTGAACTGCCCCAGCCTCCTGTCACAGAGAAGCTCACCACTGCCCAGG AAGTGCTGGCCCGTGCCCGGAGCTTGATGACACCCAAGATGGAGAAGGCCCTGAGCAACCTGGCCCTGCG CTCGGCTGAGCCCGGTAGCCCTGGGACCTCTACTCCACCACTCCCGGCCACTCCGCCAGCCACCCCACCT GCTGCCTCTCCGAGCGCCCTGAAGGGTGTGTCCCAAGCACTGCTAGAGCGGATAAGGGCCAAGGAGGTCC AGAAGCAGCTGGCAAGGATGACACGGTGCCCCGAGCAGGAGCTTCGCCTGCAGCGGTTAGAGCGTCTGCC AGAGCTGGCCCGCGTGCTGCGCAATGTCTTCGTGTCTGAGCGGAAGCCGGCACTCACTATGGAGGTGGTC TGTGCAAGGATGGTGGACAGTTGCCAAACTGCTCTGAGTCCAGGGGAGATGGAGAAACATCTGGTGCTCC TGGCAGAGTTGCTGCCGGACTGGCTCAGCCTGCATCGCATCCGCACGGATACCTACGTCAAGCTGGACAA GGCTGTTGACCTGGCTGGCCTCACTGCGAGGCTGGCCCACCACGTCCACGCCGAGGGGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208824 representing NM_026014
Red=Cloning site Green=Tags(s) MAQSRVTDFYACRRPGLTTPRAKSICLTPSPGGLVAPAFTRSSSRKRARPPAEPGSDQPAPLARRRLRLP GLDSCPSSLPEPSSPAEPSPPADPSPPADPGSPVCPSPVKRTKSTTVYVGQQPGKIPSEDSVSELQSCLR RARKLGAQARALRARVQENAVEPSTPDAKVPTEQPCVEKAPAYQRFHALAQPGLPGLVLPYKYQVLVEMF RSMDTIVSMLHNRSETVTFAKVKQGVQEMMRKRFEERNVGQIKTVYPTSYRFRQECNVPTFKDSIKRSDY QLTIEPLLGQEAGGATQLTATCLLQRRQVFRQNLVERVKEQHKVFLASLNPPMAVPDDQLTRWHPRFNVD EVPDIEPAELPQPPVTEKLTTAQEVLARARSLMTPKMEKALSNLALRSAEPGSPGTSTPPLPATPPATPP AASPSALKGVSQALLERIRAKEVQKQLARMTRCPEQELRLQRLERLPELARVLRNVFVSERKPALTMEVV CARMVDSCQTALSPGEMEKHLVLLAELLPDWLSLHRIRTDTYVKLDKAVDLAGLTARLAHHVHAEGL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026014 |
| ORF Size | 1671 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026014.3, NP_080290.3 |
| RefSeq Size | 2245 bp |
| RefSeq ORF | 1674 bp |
| Locus ID | 67177 |
| UniProt ID | Q8R4E9 |
| Gene Summary | Required for both DNA replication and mitosis. DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC). Required also for mitosis by promoting stable kinetochore-microtubule attachments (By similarity). Potential oncogene (PubMed:11850834).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204648 | Cdt1 (untagged) - Mouse chromatin licensing and DNA replication factor 1 (Cdt1), (10ug) |
CNY 2,000.00 |
|
| MR208824 | Cdt1 (Myc-DDK-tagged) - Mouse chromatin licensing and DNA replication factor 1 (Cdt1) |
CNY 5,610.00 |
|
| MR208824L3 | Lenti ORF clone of Cdt1 (Myc-DDK-tagged) - Mouse chromatin licensing and DNA replication factor 1 (Cdt1) |
CNY 7,510.00 |
|
| MR208824L4 | Lenti ORF clone of Cdt1 (mGFP-tagged) - Mouse chromatin licensing and DNA replication factor 1 (Cdt1) |
CNY 7,510.00 |
