Angptl4 (NM_020581) Mouse Tagged ORF Clone

CAT#: MG206437

  • TrueORF®

Angptl4 (tGFP-tagged) - Mouse angiopoietin-like 4 (Angptl4)

ORF Plasmid: DDK tGFP



  "NM_020581" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,140.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Arp4; Bk89; Fiaf; Hfarp; Ng27; Pgar; Pgarg; Pp1158
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG206437 representing NM_020581
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCTGCGCTCCGACAGCAGGCGCTGCCCTGGTGCTATGCGCGGCTACTGCGGGGCTTTTGAGCGCGC
AAGGGCGCCCTGCACAGCCAGAGCCACCGCGCTTTGCATCCTGGGACGAGATGAACTTGCTGGCTCACGG
GCTGCTACAGCTCGGCCATGGGCTGCGCGAACACGTGGAGCGCACCCGTGGGCAGCTGGGCGCGCTGGAG
CGCCGCATGGCTGCCTGTGGTAACGCTTGTCAGGGGCCCAAGGGAAAAGATGCACCCTTCAAAGACTCCG
AGGATAGAGTCCCTGAAGGCCAGACTCCTGAGACTCTGCAGAGTTTGCAGACTCAGCTCAAGGCTCAAAA
CAGCAAGATCCAGCAATTGTTCCAGAAGGTGGCCCAGCAGCAGAGATACCTATCAAAGCAGAATCTGAGA
ATACAGAATCTTCAGAGCCAGATAGACCTCTTGGCCCCCACGCACCTAGACAATGGAGTAGACAAGACTT
CGAGGGGAAAGAGGCTTCCCAAGATGACCCAGCTCATTGGCTTGACTCCCAACGCCACCCACTTACACAG
GCCGCCCCGGGACTGCCAGGAACTCTTCCAAGAAGGGGAGAGGCACAGTGGACTTTTCCAGATCCAGCCT
CTGGGGTCTCCACCATTTTTGGTCAACTGTGAGATGACTTCAGATGGAGGCTGGACAGTGATTCAGAGAC
GCCTGAACGGCTCTGTGGACTTCAACCAGTCCTGGGAAGCCTACAAGGATGGCTTCGGAGATCCCCAAGG
CGAGTTCTGGCTGGGCCTGGAAAAGATGCACAGCATCACAGGGAACCGAGGAAGCCAATTGGCTGTGCAG
CTCCAGGACTGGGATGGCAATGCCAAATTGCTCCAATTTCCCATCCATTTGGGGGGTGAGGACACAGCCT
ACAGCCTGCAGCTCACTGAGCCCACGGCCAATGAGCTGGGTGCCACCAATGTTTCCCCCAATGGCCTTTC
CCTGCCCTTCTCTACTTGGGACCAAGACCATGACCTCCGTGGGGACCTTAACTGTGCCAAGAGCCTCTCT
GGTGGCTGGTGGTTTGGTACCTGTAGCCATTCCAATCTCAATGGACAATACTTCCACTCTATCCCACGGC
AACGGCAGGAGCGTAAAAAGGGTATCTTCTGGAAAACATGGAAGGGCCGCTACTATCCTCTGCAGGCTAC
CACCCTGCTGATCCAGCCCATGGAGGCTACAGCAGCCTCT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG206437 representing NM_020581
Red=Cloning site Green=Tags(s)

MRCAPTAGAALVLCAATAGLLSAQGRPAQPEPPRFASWDEMNLLAHGLLQLGHGLREHVERTRGQLGALE
RRMAACGNACQGPKGKDAPFKDSEDRVPEGQTPETLQSLQTQLKAQNSKIQQLFQKVAQQQRYLSKQNLR
IQNLQSQIDLLAPTHLDNGVDKTSRGKRLPKMTQLIGLTPNATHLHRPPRDCQELFQEGERHSGLFQIQP
LGSPPFLVNCEMTSDGGWTVIQRRLNGSVDFNQSWEAYKDGFGDPQGEFWLGLEKMHSITGNRGSQLAVQ
LQDWDGNAKLLQFPIHLGGEDTAYSLQLTEPTANELGATNVSPNGLSLPFSTWDQDHDLRGDLNCAKSLS
GGWWFGTCSHSNLNGQYFHSIPRQRQERKKGIFWKTWKGRYYPLQATTLLIQPMEATAAS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020581
ORF Size 1230 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_020581.1, NP_065606.1
RefSeq Size 1858 bp
RefSeq ORF 1233 bp
Locus ID 57875
UniProt ID Q9Z1P8
Gene Summary Mediates inactivation of the lipoprotein lipase LPL, and thereby plays a role in the regulation of triglyceride clearance from the blood serum and in lipid metabolism (PubMed:15837923, PubMed:17609370, PubMed:29899519). May also play a role in regulating glucose homeostasis and insulin sensitivity (PubMed:15837923, PubMed:29899519). Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage (PubMed:14583458, PubMed:17130448, PubMed:21832056). Upon heterologous expression, inhibits the adhesion of endothelial cell to the extracellular matrix (ECM), and inhibits the reorganization of the actin cytoskeleton, formation of actin stress fibers and focal adhesions in endothelial cells that have adhered to ANGPTL4-containing ECM (in vitro) (By similarity). Depending on context, may modulate tumor-related angiogenesis (Probable).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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