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Ackr1 (NM_010045) Mouse Tagged ORF Clone
CAT＃: MG204947
- TrueORF®
Darc (tGFP-tagged) - Mouse Duffy blood group, chemokine receptor (Darc)
ORF Plasmid: DDK
"NM_010045" in other vectors (3)
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CNY 7,088.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA162249; CCBP1; CD234; Darc; Dfy; ESTM35; FY; GPD |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204947 representing NM_010045
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAACTGTCTGTATCCGGTGGAAACCCTTTCACTAGACAAGAATGGAACTCAGTTTACTTTCGACA GCTGGAATTATTCGTTTGAAGATAACTACTCCTATGAACTCTCGAGTGACTACAGCCTGACACCAGCTGC TCCCTGCTACTCCTGTAATCTGCTTGACAGGTCTTCCCTGCCCTTCTTCATGCTCACCAGTGTCCTGGGC ATGCTGGCAAGTGGCAGCATCCTCTTCGCGATTCTCAGACCTTTCTTCCACTGGCAGATTTGCCCCAGCT GGCCCATTCTGGCAGAGTTAGCAGTGGGCAGTGCCCTGTTCAGCATTGCAGTGCCCATCCTGGCACCAGG CTTACACAGCGCCCACAGCACAGCCCTATGCAACCTGGGCTACTGGGTATGGTATACTTCTGCTTTTGCC CAAGCTCTGTTGATAGGATGCTATGCTTGCCTGAATCCCAGACTGAATATTGGTCAACTCCGTGGCTTCA CCTTGGGACTCAGTGTGGGACTTTGGGGAGCAGCTGCCCTCTCAGGGCTGCCAGTCGCCCTGGCCAGTGA TGTTTACAATGGCTTCTGCACCTTTCCATCCTCCAGAGACATGGAAGCTTTGAAGTACACTCATTATGCC ATCTGTTTTACCATCTTCACTGTATTGCCACTGACTCTTTTGGCAGCCAAGGGGCTGAAGATAGCACTGA GCAAGGGGCCTGGCCCCTGGGTTAGTGTCTTGTGGATCTGGTTCATTTTCTGGTGGCCTCATGGGATGGT TCTCATATTTGATGCTTTGGTGAGGTCCAAAACTGTTCTTCTGTATACATGTCAATCCCAGAAGATTCTT GATGCGATGCTGAATGTGACAGAAGCCCTGAGTATGCTGCACTGTGTGGCTACCCCACTGCTCCTGGCCC TGTTCTGCCATCAGACCACCCGTAGATCCTTGTCCTCACTCTCCCTCCCTACAAGACAGGCTTCTCAAAT GGATGCCCTTGCAGGCAAGTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204947 representing NM_010045
Red=Cloning site Green=Tags(s) MGNCLYPVETLSLDKNGTQFTFDSWNYSFEDNYSYELSSDYSLTPAAPCYSCNLLDRSSLPFFMLTSVLG MLASGSILFAILRPFFHWQICPSWPILAELAVGSALFSIAVPILAPGLHSAHSTALCNLGYWVWYTSAFA QALLIGCYACLNPRLNIGQLRGFTLGLSVGLWGAAALSGLPVALASDVYNGFCTFPSSRDMEALKYTHYA ICFTIFTVLPLTLLAAKGLKIALSKGPGPWVSVLWIWFIFWWPHGMVLIFDALVRSKTVLLYTCQSQKIL DAMLNVTEALSMLHCVATPLLLALFCHQTTRRSLSSLSLPTRQASQMDALAGKS TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010045 |
| ORF Size | 1002 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010045.2, NP_034175.2 |
| RefSeq Size | 1158 bp |
| RefSeq ORF | 1005 bp |
| Locus ID | 13349 |
| UniProt ID | Q9QUI6 |
| Gene Summary | Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Has a promiscuous chemokine-binding profile, interacting with inflammatory chemokines of both the CXC and the CC subfamilies but not with homeostatic chemokines. Acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1 and TARC. May regulate chemokine bioavailability and, consequently, leukocyte recruitment through two distinct mechanisms: when expressed in endothelial cells, it sustains the abluminal to luminal transcytosis of tissue-derived chemokines and their subsequent presentation to circulating leukocytes; when expressed in erythrocytes, serves as blood reservoir of cognate chemokines but also as a chemokine sink, buffering potential surges in plasma chemokine levels (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR204947 | Darc (Myc-DDK-tagged) - Mouse Duffy blood group, chemokine receptor (Darc) |
CNY 5,488.00 |
|
| MR204947L3 | Lenti ORF clone of Darc (Myc-DDK-tagged) - Mouse Duffy blood group, chemokine receptor (Darc) |
CNY 5,890.00 |
|
| MR204947L4 | Lenti ORF clone of Darc (mGFP-tagged) - Mouse Duffy blood group, chemokine receptor (Darc) |
CNY 5,890.00 |
