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Eif2s1 (NM_026114) Mouse Tagged ORF Clone
CAT＃: MG204498
- TrueORF®
Eif2s1 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 1 alpha (Eif2s1)
ORF Plasmid: DDK
"NM_026114" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 35kDa; 0910001O23Rik; 2410026C18Rik; Eif2a; eIF2alpha |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204498 representing NM_026114
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGGGCTAAGTTGTAGATTTTATCAACACAAATTTCCTGAGGTGGAAGATGTAGTGATGGTGAATG TAAGATCCATTGCTGAAATGGGGGCCTATGTCAGCTTGTTGGAATATAATAACATTGAAGGCATGATTCT TCTTAGTGAATTATCCAGACGACGTATCCGTTCTATAAACAAACTGATCCGAATTGGCAGAAATGAATGT GTTGTTGTCATTAGAGTGGATAAAGAAAAAGGATATATAGATTTGTCAAAAAGAAGAGTTTCTCCAGAGG AAGCAATCAAATGTGAGGACAAATTCACAAAATCCAAAACTGTTTATAGCATTCTTCGCCATGTTGCTGA GGTATTAGAATATACCAAGGATGAGCAGCTGGAGAGCCTGTTCCAGAGGACTGCCTGGGTCTTCGATGAC AAGTACAAGAGACCTGGATACGGTGCCTACGATGCTTTTAAGCATGCAGTCTCAGACCCATCTATCTTGG ATAGTTTAGATTTGAATGAAGATGAAAGAGAAGTACTCATTAATAATATCAATAGGCGTTTGACCCCACA AGCGGTCAAAATTCGAGCAGATATTGAAGTAGCTTGCTATGGTTATGAAGGCATTGATGCTGTAAAAGAA GCCCTGAGGGCAGGTTTGAATTGTTCTACAGAAACCATGCCCATCAAGATTAATCTAATAGCTCCACCCA GGTATGTGATGACAACAACGACCCTGGAGAGGACAGAAGGCCTGTCTGTCCTCAATCAGGCTATGGCAGT TATCAAAGAGAAGATCGAGGAGAAGAGGGGCGTCTTCAATGTTCAGATGGAGCCCAAAGTGGTCACAGAT ACAGATGAGACTGAACTTGCAAGGCAGCTGGAACGGCTGGAGAGAGAAAATGCAGAAGTGGATGGAGATG ATGATGCAGAAGAAATGGAAGCCAAAGCTGAAGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204498 representing NM_026114
Red=Cloning site Green=Tags(s) MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNEC VVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDD KYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKE ALRAGLNCSTETMPIKINLIAPPRYVMTTTTLERTEGLSVLNQAMAVIKEKIEEKRGVFNVQMEPKVVTD TDETELARQLERLERENAEVDGDDDAEEMEAKAED TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026114 |
| ORF Size | 945 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026114.3 |
| RefSeq Size | 3153 bp |
| RefSeq ORF | 948 bp |
| Locus ID | 13665 |
| UniProt ID | Q6ZWX6 |
| Gene Summary | Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201485 | Eif2s1 (untagged) - Mouse eukaryotic translation initiation factor 2, subunit 1 alpha (Eif2s1), (10ug) |
CNY 2,000.00 |
|
| MR204498 | Eif2s1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 1 alpha (Eif2s1) |
CNY 1,416.00 |
|
| MR204498L3 | Lenti ORF clone of Eif2s1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 1 alpha (Eif2s1) |
CNY 4,750.00 |
|
| MR204498L4 | Lenti ORF clone of Eif2s1 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2, subunit 1 alpha (Eif2s1) |
CNY 4,750.00 |
