Sfrp2 (NM_009144) Mouse Tagged ORF Clone

CAT#: MG204070

  • TrueORF®

Sfrp2 (tGFP-tagged) - Mouse secreted frizzled-related protein 2 (Sfrp2)

ORF Plasmid: DDK tGFP



  "NM_009144" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 2,850.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AI851596; Sdf5
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG204070 representing NM_009144
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCGGGGCCCTGCCTCGCTGCTGCTGCTAGTCCTCGCCTCGCACTGCTGCCTGGGCTCGGCGCGTG
GGCTCTTCCTCTTCGGCCAGCCCGACTTCTCCTACAAGCGCAGCAACTGCAAGCCCATCCCCGCCAACCT
GCAGCTGTGCCACGGCATCGAGTACCAGAACATGCGGCTGCCCAACCTGCTGGGCCACGAGACCATGAAG
GAGGTGCTGGAGCAGGCGGGCGCCTGGATTCCGCTGGTCATGAAGCAGTGCCACCCGGACACCAAGAAGT
TCCTGTGCTCGCTCTTCGCCCCTGTCTGTCTCGACGACCTAGATGAGACCATCCAGCCGTGTCACTCGCT
CTGCGTGCAGGTGAAGGACCGCTGCGCCCCGGTCATGTCCGCCTTCGGCTTCCCCTGGCCAGACATGCTG
GAGTGCGACCGTTTCCCGCAGGACAACGACCTCTGCATCCCCCTCGCTAGTAGCGACCACCTCCTGCCGG
CCACAGAGGAAGCTCCCAAGGTGTGTGAAGCCTGCAAAACCAAGAATGAGGACGACAACGACATCATGGA
AACCCTTTGTAAAAATGACTTCGCACTGAAAATCAAAGTGAAGGAGATAACGTACATCAACAGAGACACC
AAGATCATCCTGGAGACAAAGAGCAAGACCATTTACAAGCTGAACGGCGTGTCCGAAAGGGACCTGAAGA
AATCCGTGCTGTGGCTCAAAGACAGCCTGCAGTGCACCTGTGAGGAGATGAACGACATCAACGCTCCGTA
TCTGGTCATGGGACAGAAGCAGGGCGGCGAGCTGGTGATCACCTCCGTGAAACGGTGGCAGAAGGGCCAG
AGAGAGTTCAAGCGCATCTCCCGCAGCATCCGCAAGCTGCAATGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG204070 representing NM_009144
Red=Cloning site Green=Tags(s)

MPRGPASLLLLVLASHCCLGSARGLFLFGQPDFSYKRSNCKPIPANLQLCHGIEYQNMRLPNLLGHETMK
EVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCVQVKDRCAPVMSAFGFPWPDML
ECDRFPQDNDLCIPLASSDHLLPATEEAPKVCEACKTKNEDDNDIMETLCKNDFALKIKVKEITYINRDT
KIILETKSKTIYKLNGVSERDLKKSVLWLKDSLQCTCEEMNDINAPYLVMGQKQGGELVITSVKRWQKGQ
REFKRISRSIRKLQC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_009144
ORF Size 885 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_009144.2
RefSeq Size 2028 bp
RefSeq ORF 888 bp
Locus ID 20319
UniProt ID P97299
Gene Summary Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...