Ccdc85b (NM_198616) Mouse Tagged ORF Clone
CAT#: MG202077
- TrueORF®
Ccdc85b (tGFP-tagged) - Mouse coiled-coil domain containing 85B (Ccdc85b)
ORF Plasmid: DDK
"NM_198616" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AI842788; BB115872 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG202077 representing NM_198616
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCGAAGCAGGCGGCCTGGAGGAGCTGACGGACGAGGAGATGGCGGCGCTGGGTAAGGAGGAGC TGGTGCGGCGCCTGCGGCGGGAGGAGGCGGCGCGCCTGGCAGCGCTAGTGCAGCGCGGCCGCCTGATGCA GGAGGTGAATCGGCAGCTGCAGGGTCACCTGGGCGAGATCCGTGAGCTCAAACAGCTCAACCGGCGCCTG CAGGCGGAGAACCGGGAGCTGCGCGACCTCTGCTGCTTTCTGGACTCGGAGCGCCAACGAGGACGGCGTG CGGCGCGCCAGTGGCAGCTCTTTGGGACCCAAGCATCCCGGGCGGTGCGCGAGGACCTGGGTGGCTGTTG GCAGAAGCTAGCGGAGCTGGAAGGCCGCCAGGAGGAGCTGCTGCGGGAAAATCTGGCCCTTAAGGAGCTT TGCTTAGCACTGGGCGAAGAGTGGGGCCCCCGCGGTGGCCCCGGCGGCGCAGTGGGCTCAGGTGCTGGGC CCACACCCGAGCTCGCCCTGCCACCGTGCGGGCCCCGCGACCTAGGCGATGGAAGTTCCAGCACTGGCAG TGTGGGCAGCCCGGATCAGTTGCCCCTAGCCTGCTCCCCTGATGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202077 representing NM_198616
Red=Cloning site Green=Tags(s) MEAEAGGLEELTDEEMAALGKEELVRRLRREEAARLAALVQRGRLMQEVNRQLQGHLGEIRELKQLNRRL QAENRELRDLCCFLDSERQRGRRAARQWQLFGTQASRAVREDLGGCWQKLAELEGRQEELLRENLALKEL CLALGEEWGPRGGPGGAVGSGAGPTPELALPPCGPRDLGDGSSSTGSVGSPDQLPLACSPDD TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_198616 |
ORF Size | 606 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_198616.4, NP_941018.1 |
RefSeq Size | 1071 bp |
RefSeq ORF | 609 bp |
Locus ID | 240514 |
UniProt ID | Q6PDY0 |
Gene Summary | Functions as a transcriptional repressor. May inhibit the activity of CTNNB1 in a TP53-dependent manner and thus regulate cell growth. May function in adipocyte differentiation, negatively regulating mitotic clonal expansion (PubMed:15644333, PubMed:22666460).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207927 | Ccdc85b (untagged) - Mouse coiled-coil domain containing 85B (Ccdc85b), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
MR202077 | Ccdc85b (Myc-DDK-tagged) - Mouse coiled-coil domain containing 85B (Ccdc85b), transcript variant 1 |
CNY 1,416.00 |
|
MR202077L3 | Lenti ORF clone of Ccdc85b (Myc-DDK-tagged) - Mouse coiled-coil domain containing 85B (Ccdc85b), transcript variant 1 |
CNY 4,750.00 |
|
MR202077L4 | Lenti ORF clone of Ccdc85b (mGFP-tagged) - Mouse coiled-coil domain containing 85B (Ccdc85b), transcript variant 1 |
CNY 4,750.00 |
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