PD-L1 (CD274) Mouse Monoclonal Antibody [Clone ID: UMAB228]

Specifications

Product Data
Clone Name	UMAB228
Applications	IHC, WB
Recommend Dilution	WB 1:500, IHC 1:300
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 19-290 of human CD274 (PD-L1) (NP_054862) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	33.28 kDa
Gene Name	CD274 molecule
Database Link	NP_054862 Entrez Gene 29126 Human UniProt ID Q9NZQ7
Synonyms	B7-H; B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs)

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