RANTES (CCL5) Mouse Monoclonal Antibody [Clone ID: 5B3-D5]

Specifications

Product Data
Clone Name	5B3-D5
Applications	ELISA, FN, IF, IHC, IP, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human RANTES (100 ng/ml), a concentration of 3.0-5.0 µg/ml of this antibody is required. Sandwich ELISA: In a Sandwich ELISA (assuming 100 µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 500 pg/ml of recombinant Human RANTES when used with Biotinylated antigen affinity purified anti-Human RANTES (PP1064B1 or PP1064B2) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml. Western Blot: To detect Human RANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human RANTES is 0.25-2.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions. Immunohistochemistry on Frozen Sections.   Immunohistochemistry on Paraffin Sections: 10 µg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 citrate buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia. Immunofluorescence: This antibody (Lot #057E172) stained PBMC. The primary antibody was incubated at 10.0 mg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	E. Coli derived recombinant Human Rantes (Cat.-No PA123)
Specificity	Recognizes Human RANTES chemokine. Other species not tested.
Formulation	PBS without preservatives State: Azide Free State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine ligand 5
Database Link	Entrez Gene 6352 Human UniProt ID P13501
Background	RANTES (Regulated-on-activation-normal-T-cell-expressed-and-secreted) is a member of the "intercrine/chemokine" family of cytokines, has been previously shown to be chemotactic for monocytes and T cells of memory/helper phenotype. RANTES has been shown to cause the release of histamine from basophils and activates eosinophils. It also binds to CCR1, CCR3, CCR4 and CCR5. This CC-chemokine enhances antigen-specific T helper (Th) type-1 responses against HIV-1. CC chemokines are small inducible proteins that are related to interleukin 8. In lung adenocarcinoma, RANTES is a predictor of survival while that of interleukin (IL)-8 is associated with a poor prognosis. In several models, tumorigenesis is abolished by RANTES, while it is facilitated by IL-8. Site-directed mutagenesis indicated that regulation of RANTES promoter activity required two nuclear factor (NF)-kappaB response elements but not its activator protein (AP)-1 binding sites.
Synonyms	C-C motif chemokine 5, D17S136E, SCYA5, Small-inducible cytokine A5, SIS-delta, EoCP
Reference Data

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