IL7 Rabbit Polyclonal Antibody

CAT#: PP1029B2

IL7 rabbit polyclonal antibody, Biotin

Conjugation: Unconjugated Biotin



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CNY 4,790.00


货期*
5周

规格
    • 50 ug

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Specifications

Product Data
Applications ELISA, WB
Recommend Dilution ELISA: To detect hIL-7 by direct ELISA (using 100 ml/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-7.
Western Blot: To detect hIL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>98%) recombinant hIL-7 (human IL-7).
Specificity Human IL-7
Formulation PBS, pH 7.2 without preservatives.
Label: Biotin
State: Lyophilized purified Ig fraction.
Label: conjugated
Reconstitution Method Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification Affinity chromatography.
Conjugation Biotin
Storage Condition Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name interleukin 7
Background Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.
Synonyms IL-7
Note Centrifuge vial prior to opening!
Reference Data
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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