Drebrin (DBN1) (324-343) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, IP, WB
Recommend Dilution	Immunofluorescence / Immunocytochemistry (assay dependent). Immunoblotting (assay dependent). Immunoprecipitation (assay dependent). Immunohistochemistry on Frozen Paraformaldehyde-Fixed Sections: 1/100. Immunohistochemistry on Paraffin Sections: 1/100 (Microwave treatment is recommended) Incubation Time: 1h at RT, extended with paraffin. Immunohistochemistry on Cytological Material.
Reactivity	Bovine, Human, Mouse, Rat
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Synthetic Human peptide (aa 324-343) coupled to KLH.
Specificity	The antiserum specifically reacts with Drebrin isoforms E2 and A, widespread actin-associating proteins of 70 kD (SDS-PAGE mobility shows Mr 120,000). Reacts with PLC, U333, HUVEC, HaCat, HeLa, MCF-7, SV-80; B1; B16 (Mouse melanoma cells).
Formulation	State: Serum State: Liquid Serum Stabilizer: 0.5% BSA Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	drebrin 1
Database Link	Entrez Gene 1627 Human UniProt ID Q16643
Background	Drebrin has originally been described only in neuronal cells, but has been recently found also in the actin-rich lamellipodia and filopodia of motile cells. It is enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells. Positive staining was found in many tissues, including diverse epithelia and carcinoma (e.g. in epidermis basal cells are positive, whereas overlying cells are negative; also positive: regenerating epithelium during wound healing; basal cell carcinoma; skin melanoma), specific types of endothelia and smooth muscle; especially prominent in the mesangial cells of renal glomeruli and in the Sertoli cells of testis. Also positive: epithelia of hair follicles and eccrine sweat glands. Consistently negative were, however, hepatocytes and cross-striated muscle.
Synonyms	DBN1, DBN-1, D0S117E, DKFZp434D064,drebrin 1
Reference Data

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