Cytokeratin 9 (KRT9) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Immunoblotting: 1/5000-1/10,000 for Western Blot (ECL). Immunohistochemistry on Frozen Sections: 1/100-1/200. Immunohistochemistry on Paraffin Embedded Sections: 1/50 (Microwave retrieval method is recommended). Incubation Time: 1 h at RT, extended with Paraffin.
Reactivity	Bovine, Hamster, Human, Mouse, Rat, Xenopus
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	KLH conjugated Synthetic peptide corresponding to amino acids 450-477 of Human keratin K9
Specificity	The antiserum represents an excellent marker to study palmoplantar epidermal distribution and differentiation, specifically reactive in the middle/upper suprabasal layers (stratum spinosum/ granulosum) of the epidermis of palm and sole. K9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). K9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts. No labelling has been found in epithelial cells of other stratified epithelia such as oesophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia. Negative tissues include: muscle, liver and duodenum (See references 1 and 2).
Formulation	State: Serum State: Liquid Serum Stabilizer: 0.5% BSA Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	keratin 9
Database Link	Entrez Gene 3857 Human UniProt ID P35527
Background	Acidic keratin K9 (MW 62,129; formerly also designated cytokeratin 9), expressed in the palmoplantar epidermis.
Synonyms	Cytokeratin 9, KRT9, CK9, Keratin-9, K9
Reference Data

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