Klotho Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Human Klotho by Direct ELISA (using 100μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Klotho.  Sandwich ELISA: To detect Human Klotho by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with purified anti-Klotho (Cat.-No AP32851BT-S or AP32851BT-N) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Klotho.  Western Blot: To detect Human Klotho by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Klotho is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) CHO cells derived recombinant Human Klotho (Cat.-No AR08005PU)
Specificity	This antibody recognizes Human Klotho. Other species not tested.
Formulation	PBS, pH 7.2 Label: Biotin State: Lyophilized (0.2µ Sterile filtered) purified protein
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month  or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	klotho
Database Link	Entrez Gene 9365 Human UniProt ID Q9UEF7
Background	Klotho is a glycosylated protein that plays an important role in the regulation of phosphate and calcium homeostasis. Human Klotho exists in both membrane bound and secreted forms, and is predominantly expressed in the kidney convoluted tubules, and to a lesser extent, in the brain, reproductive organs, endocrine glands, urinary bladder, skeletal muscle, placenta, and colon,. The full length transmembrane form has a large extracellular domain composed of two homologous subunits termed KL1 and KL2, which contain 516 and 439 amino acid residues, respectively, The predominant circulating form, which is derived from alternative RNA splicing, contains the KL1 subunit and constitutes the N-terminal sequence of transmembrane Klotho. A third Klotho protein of about 128 kDa has been identified in the blood and cerebrospinal fluid. This circulating protein arises from the action of an as yet unidentified protease which cleaves transmembrane Klotho just above and/or within the plasma membrane. Klotho has been shown to play a key role in the signaling cascade of fibroblast growth factor-23 (FGF-23), a bone derived hormone that acts in the kidney to inhibit phosphate reabsorption and vitamin D biosynthesis. Klotho promotes FGF-23 signaling through binding to FGFRI (IIIc) which converts this canonical FGF receptor into a specific receptor for FGF-23. In the absence of Klotho the function of FGF-23 is literally abolished.
Synonyms	KL, Klotho, EC 3.2.1.31
Reference Data

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