MSH2 Mouse Monoclonal Antibody [Clone ID: G219-1129]

Specifications

Product Data
Clone Name	G219-1129
Applications	IHC
Recommend Dilution	Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/501/100 Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended. Use Polymer anti Mouse/Rabbit IgG as a detection system. Positive Control: Colon carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	BALB/C mice were injected with recombinant Human MSH2 protein.
Specificity	Recognizes MSH2 Cellular Localization: Nuclear.
Formulation	State: Supernatant State: Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	mutS homolog 2
Database Link	Entrez Gene 4286 HumanEntrez Gene 4436 Human UniProt ID P43246
Background	Germline mutations in human mismatch repair genes (hMSH2, hMSH6, hMLH1, hPMS2) account for majority of the hereditary non-polyposis colorectal carcinoma (HPNCC). CpG dinucleotides in the hMSH2 and hMLH1 genes are hotspots for HNPCC mutations. These mutations cause a mismatch repair deficiency resulting in a mutator phenotype where the replication errors are not repaired. Microsatellites / simple repeatative sequences are prone to this type of replication errors and instability of these microsatellites correlates with the occurance of HPNCC. hMSH2 binds to another MutS homolog protein GTBP to form a heterodimeric complex called hMutSbeta, which binds to insertion/deletion loops in DNA.
Synonyms	DNA mismatch repair protein Msh2, MutS protein homolog 2
Reference Data
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Melanogenesis, Melanoma, Pathways in cancer

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.