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DHX8 (NM_001302623) Human Tagged ORF Clone
CAT＃: RG240028
- TrueORF®
DHX8 (tGFP-tagged) - Human DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), transcript variant 2
ORF Plasmid: DDK
"NM_001302623" in other vectors (2)
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CNY 12,160.00
货期*
2周
规格
Product images
经常一起买 (5)
Specifications
| Product Data | |
| Tag | TurboGFP |
| Synonyms | DDX8; Dhr2; HRH1; PRP22; PRPF22 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG240028 representing NM_001302623.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTGTGGCTGTAGCCATGGCGGGAGCCTTAATCGGGTCGGAGCCAGGCCCCGCGGAAGAACTTGCC AAACTCGAGTACCTGTCTTTGGTGTCAAAGGTTTGCACTGAGCTGGACAATCACTTGGGGATCAACGAC AAGGACCTTGCTGAATTTGTGATCAGTCTTGCTGAGAAAAATACCACCTTTGATACTTTTAAGGCTTCT CTCGTCAAAAATGGTGCAGAATTTACGGATTCTCTTATTAGTAACTTGCTGCGTCTCATACAAACCATG CGGCCTCCAGCGAAGCCTTCCACTAGCAAAGATCCAGTTGTTAAACCTAAAACAGAAAAAGAAAAGCTG AAGGAACTCTTCCCAGTCCTTTGCCAACCGGACAACCCTTCTGTTCGGACCATGTTGGATGAAGATGAT GTGAAAGTTGCTGTGGATGTCCTGAAAGAACTGGAAGCTTTAATGCCCAGCGCAGCAGGCCAGGAGAAG CAAAGAGATGCTGAACACCGGGACAGGACAAAGAAGAAGAAGCGGAGTCGAAGCCGAGATCGAAACCGA GATCGAGACAGAGATAGGGAACGAAACCGAGATAGAGACCACAAGCGGAGACACCGATCCCGCTCTCGA TCACGTTCCAGGACCCGGGAGAGGAATAAAGTGAAGTCTAGATATCGGTCCAGGAGCAGGAGTCAGAGT CCCCCCAAAGACCGGAAGGACCGGGACAAATATGGAGAGCGGAATCTGGATAGATGGCGGGATAAGCAT GTGGACCGCCCTCCTCCAGAAGAGCCCACCATTGGTGACATTTATAATGGCAAAGTTACCAGCATCATG CAGTTTGGTTGCTTTGTGCAGCTGGAAGGACTAAGGAAGCGGTGGGAAGGCCTGGTGCACATCTCTGAG CTCCGGCGGGAGGGTCGTGTGGCCAATGTAGCTGATGTCGTGAGCAAAGGCCAGAGGGTCAAAGTCAAA GTGCTGTCCTTCACTGGGACCAAGACCAGCCTGAGCATGAAGGATGTGGATCAAGAGACTGGAGAAGAT CTAAACCCAAATAGACGGCGAAATCTTGTCGGGGAGACCAATGAGGAGACCTCAATGCGGAATCCTGAT AGACCCACTCACTTGTCCCTTGTCAGTGCTCCTGAAGTAGAGGACGACTCACTGGAACGCAAGCGCCTC ACCCGAATCTCTGACCCAGAGAAGTGGGAGATCAAACAGATGATTGCTGCCAATGTCCTTTCCAAAGAA GAATTTCCAGACTTTGATGAAGAGACTGGCATTCTCCCTAAGGTGGATGATGAAGAAGATGAGGACCTT GAGATTGAATTGGTTGAGGAAGAGCCTCCATTCCTGAGAGGGCACACTAAGCAAAGCATGGACATGAGC CCCATTAAAATTGTCAAGAACCCAGACGGCTCCCTCTCCCAAGCAGCAATGATGCAGAGTGCCTTGGCC AAAGAAAGGCGGGAACTCAAACAGGCCCAGCGGGAAGCTGAGATGGATTCTATTCCCATGGGACTCAAC AAACACTGGGTTGACCCTCTGCCTGATGCGGAAGGCAGACAGATTGCTGCCAACATGAGGGGTATTGGG ATGATGCCCAATGATATTCCTGAGTGGAAGAAGCATGCCTTTGGGGGCAACAAAGCCTCTTACGGAAAA AAGACCCAGATGTCAATCCTTGAGCAGAGGGAGAGCCTGCCCATCTACAAACTGAAGGAGCAATTGGTC CAGGCCGTCCATGACAATCAGATCCTGATTGTCATTGGTGAGACAGGATCTGGAAAGACAACACAGATC ACCCAGTACCTGGCGGAGGCAGGCTACACTTCCAGGGGCAAGATTGGGTGTACCCAGCCCAGAAGAGTG GCAGCTATGTCGGTGGCCAAAAGAGTGTCAGAGGAGTTTGGTTGTTGCTTAGGCCAAGAGGTGGGCTAC ACCATTCGATTTGAGGACTGCACTAGCCCTGAAACAGTCATCAAGTACATGACAGATGGGATGTTGCTT AGAGAGTGCTTGATTGACCCTGACCTCACTCAGTACGCGATCATCATGTTGGACGAGGCACATGAGAGG ACAATTCACACTGATGTGCTCTTTGGATTGTTGAAAAAGACAGTTCAGAAACGGCAGGACATGAAGCTG ATTGTCACCTCAGCCACCTTGGATGCAGTGAAGTTTTCTCAATACTTCTATGAAGCTCCCATTTTCACC ATCCCAGGTCGAACATATCCAGTGGAAATACTGTACACAAAGGAACCTGAGACAGATTATCTGGATGCC AGCCTGATTACTGTTATGCAGATTCATTTAACAGAACCACCAGGTGATATCCTGGTCTTCCTGACTGGT CAGGAAGAAATTGATACTGCTTGTGAGATCCTGTATGAAAGAATGAAATCCCTGGGACCTGATGTTCCA GAGTTAATTATCCTCCCAGTGTACTCTGCTCTTCCCAGTGAGATGCAGACCCGAATCTTTGACCCAGCT CCACCAGGCAGCAGAAAGGTTGTGATTGCCACCAATATCGCAGAGACATCGCTGACTATTGATGGTATC TACTATGTGGTGGACCCAGGATTCGTGAAACAGAAAGTTTACAATTCCAAGACAGGGATTGACCAGCTC GTGGTGACGCCTATTTCTCAGGCTCAGGCAAAGCAACGAGCTGGCAGAGCTGGGAGAACAGGCCCAGGG AAGTGTTACAGGTTGTACACAGAACGTGCCTACCGAGATGAAATGCTGACCACCAACGTGCCGGAAATC CAGAGAACCAACTTAGCAAGCACAGTGCTGTCACTCAAGGCCATGGGTATCAATGATCTGCTGTCCTTT GATTTCATGGATGCCCCACCTATGGAAACTTTGATCACAGCCATGGAGCAGCTGTACACACTGGGGGCC CTGGATGACGAGGGCCTGCTCACTCGCTTGGGCCGCCGGATGGCAGAGTTCCCTCTGGAGCCAATGCTA TGCAAAATGCTCATCATGTCTGTGCATCTGGGCTGCAGTGAGGAAATGCTGACCATTGTATCCATGCTG TCTGTGCAGAACGTCTTCTATAGGCCCAAGGATAAACAAGCCCTTGCAGATCAGAAGAAGGCCAAATTC CACCAGACTGAAGGGGACCACCTCACCCTGCTAGCTGTGTACAACTCCTGGAAGAACAACAAGTTCTCC AACCCATGGTGCTATGAGAACTTTATCCAGGCTCGTTCCCTGCGCCGGGCCCAGGACATTCGCAAGCAG ATGTTAGGCATAATGGACAGACACAAGCTGGATGTTGTTTCCTGTGGCAAGTCCACAGTCCGAGTGCAG AAGGCCATCTGCAGTGGGTTCTTCCGTAATGCTGCCAAGAAAGACCCGCAGGAGGGTTACCGGACACTG ATCGACCAGCAGGTGGTCTATATCCATCCTTCCAGTGCCCTCTTCAACAGACAGCCAGAATGCCCAAAG CACTTCCTCCCTGTGGTTGCTGTAGCTGTCTCATTGGAGCAGTGCCTCTCCAAGTTTGAGGATTTGAAC AAAGAACTGGGACTGGTGACTTGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG240028
Blue=ORF Red=Cloning site Green=Tag(s) MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKAS LVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDD VKVAVDVLKELEALMPSAAGQEKQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSR SRSRTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIM QFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGED LNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKE EFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALA KERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGK KTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRV AAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHER TIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDA SLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPA PPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPG KCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGA LDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKF HQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQ KAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPECPKHFLPVVAVAVSLEQCLSKFEDLN KELGLVTC TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001302623 |
| ORF Size | 3543 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001302623.2 |
| RefSeq Size | 3875 bp |
| RefSeq ORF | 3546 bp |
| Locus ID | 1659 |
| UniProt ID | Q14562 |
| Protein Pathways | Spliceosome |
| MW | 134.5 kDa |
| Gene Summary | This gene is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
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