DHX8 (NM_001302623) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DDX8; Dhr2; HRH1; PRP22; PRPF22
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240028 representing NM_001302623 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGGCTGTAGCCATGGCGGGAGCCTTAATCGGGTCGGAGCCAGGCCCCGCGGAAGAACTTGCCA AACTCGAGTACCTGTCTTTGGTGTCAAAGGTTTGCACTGAGCTGGACAATCACTTGGGGATCAACGACAA GGACCTTGCTGAATTTGTGATCAGTCTTGCTGAGAAAAATACCACCTTTGATACTTTTAAGGCTTCTCTC GTCAAAAATGGTGCAGAATTTACGGATTCTCTTATTAGTAACTTGCTGCGTCTCATACAAACCATGCGGC CTCCAGCGAAGCCTTCCACTAGCAAAGATCCAGTTGTTAAACCTAAAACAGAAAAAGAAAAGCTGAAGGA ACTCTTCCCAGTCCTTTGCCAACCGGACAACCCTTCTGTTCGGACCATGTTGGATGAAGATGATGTGAAA GTTGCTGTGGATGTCCTGAAAGAACTGGAAGCTTTAATGCCCAGCGCAGCAGGCCAGGAGAAGCAAAGAG ATGCTGAACACCGGGACAGGACAAAGAAGAAGAAGCGGAGTCGAAGCCGAGATCGAAACCGAGATCGAGA CAGAGATAGGGAACGAAACCGAGATAGAGACCACAAGCGGAGACACCGATCCCGCTCTCGATCACGTTCC AGGACCCGGGAGAGGAATAAAGTGAAGTCTAGATATCGGTCCAGGAGCAGGAGTCAGAGTCCCCCCAAAG ACCGGAAGGACCGGGACAAATATGGAGAGCGGAATCTGGATAGATGGCGGGATAAGCATGTGGACCGCCC TCCTCCAGAAGAGCCCACCATTGGTGACATTTATAATGGCAAAGTTACCAGCATCATGCAGTTTGGTTGC TTTGTGCAGCTGGAAGGACTAAGGAAGCGGTGGGAAGGCCTGGTGCACATCTCTGAGCTCCGGCGGGAGG GTCGTGTGGCCAATGTAGCTGATGTCGTGAGCAAAGGCCAGAGGGTCAAAGTCAAAGTGCTGTCCTTCAC TGGGACCAAGACCAGCCTGAGCATGAAGGATGTGGATCAAGAGACTGGAGAAGATCTAAACCCAAATAGA CGGCGAAATCTTGTCGGGGAGACCAATGAGGAGACCTCAATGCGGAATCCTGATAGACCCACTCACTTGT CCCTTGTCAGTGCTCCTGAAGTAGAGGACGACTCACTGGAACGCAAGCGCCTCACCCGAATCTCTGACCC AGAGAAGTGGGAGATCAAACAGATGATTGCTGCCAATGTCCTTTCCAAAGAAGAATTTCCAGACTTTGAT GAAGAGACTGGCATTCTCCCTAAGGTGGATGATGAAGAAGATGAGGACCTTGAGATTGAATTGGTTGAGG AAGAGCCTCCATTCCTGAGAGGGCACACTAAGCAAAGCATGGACATGAGCCCCATTAAAATTGTCAAGAA CCCAGACGGCTCCCTCTCCCAAGCAGCAATGATGCAGAGTGCCTTGGCCAAAGAAAGGCGGGAACTCAAA CAGGCCCAGCGGGAAGCTGAGATGGATTCTATTCCCATGGGACTCAACAAACACTGGGTTGACCCTCTGC CTGATGCGGAAGGCAGACAGATTGCTGCCAACATGAGGGGTATTGGGATGATGCCCAATGATATTCCTGA GTGGAAGAAGCATGCCTTTGGGGGCAACAAAGCCTCTTACGGAAAAAAGACCCAGATGTCAATCCTTGAG CAGAGGGAGAGCCTGCCCATCTACAAACTGAAGGAGCAATTGGTCCAGGCCGTCCATGACAATCAGATCC TGATTGTCATTGGTGAGACAGGATCTGGAAAGACAACACAGATCACCCAGTACCTGGCGGAGGCAGGCTA CACTTCCAGGGGCAAGATTGGGTGTACCCAGCCCAGAAGAGTGGCAGCTATGTCGGTGGCCAAAAGAGTG TCAGAGGAGTTTGGTTGTTGCTTAGGCCAAGAGGTGGGCTACACCATTCGATTTGAGGACTGCACTAGCC CTGAAACAGTCATCAAGTACATGACAGATGGGATGTTGCTTAGAGAGTGCTTGATTGACCCTGACCTCAC TCAGTACGCGATCATCATGTTGGACGAGGCACATGAGAGGACAATTCACACTGATGTGCTCTTTGGATTG TTGAAAAAGACAGTTCAGAAACGGCAGGACATGAAGCTGATTGTCACCTCAGCCACCTTGGATGCAGTGA AGTTTTCTCAATACTTCTATGAAGCTCCCATTTTCACCATCCCAGGTCGAACATATCCAGTGGAAATACT GTACACAAAGGAACCTGAGACAGATTATCTGGATGCCAGCCTGATTACTGTTATGCAGATTCATTTAACA GAACCACCAGGTGATATCCTGGTCTTCCTGACTGGTCAGGAAGAAATTGATACTGCTTGTGAGATCCTGT ATGAAAGAATGAAATCCCTGGGACCTGATGTTCCAGAGTTAATTATCCTCCCAGTGTACTCTGCTCTTCC CAGTGAGATGCAGACCCGAATCTTTGACCCAGCTCCACCAGGCAGCAGAAAGGTTGTGATTGCCACCAAT ATCGCAGAGACATCGCTGACTATTGATGGTATCTACTATGTGGTGGACCCAGGATTCGTGAAACAGAAAG TTTACAATTCCAAGACAGGGATTGACCAGCTCGTGGTGACGCCTATTTCTCAGGCTCAGGCAAAGCAACG AGCTGGCAGAGCTGGGAGAACAGGCCCAGGGAAGTGTTACAGGTTGTACACAGAACGTGCCTACCGAGAT GAAATGCTGACCACCAACGTGCCGGAAATCCAGAGAACCAACTTAGCAAGCACAGTGCTGTCACTCAAGG CCATGGGTATCAATGATCTGCTGTCCTTTGATTTCATGGATGCCCCACCTATGGAAACTTTGATCACAGC CATGGAGCAGCTGTACACACTGGGGGCCCTGGATGACGAGGGCCTGCTCACTCGCTTGGGCCGCCGGATG GCAGAGTTCCCTCTGGAGCCAATGCTATGCAAAATGCTCATCATGTCTGTGCATCTGGGCTGCAGTGAGG AAATGCTGACCATTGTATCCATGCTGTCTGTGCAGAACGTCTTCTATAGGCCCAAGGATAAACAAGCCCT TGCAGATCAGAAGAAGGCCAAATTCCACCAGACTGAAGGGGACCACCTCACCCTGCTAGCTGTGTACAAC TCCTGGAAGAACAACAAGTTCTCCAACCCATGGTGCTATGAGAACTTTATCCAGGCTCGTTCCCTGCGCC GGGCCCAGGACATTCGCAAGCAGATGTTAGGCATAATGGACAGACACAAGCTGGATGTTGTTTCCTGTGG CAAGTCCACAGTCCGAGTGCAGAAGGCCATCTGCAGTGGGTTCTTCCGTAATGCTGCCAAGAAAGACCCG CAGGAGGGTTACCGGACACTGATCGACCAGCAGGTGGTCTATATCCATCCTTCCAGTGCCCTCTTCAACA GACAGCCAGAATGCCCAAAGCACTTCCTCCCTGTGGTTGCTGTAGCTGTCTCATTGGAGCAGTGCCTCTC CAAGTTTGAGGATTTGAACAAAGAACTGGGACTGGTGACTTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240028 representing NM_001302623 Red=Cloning site Green=Tags(s) MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASL VKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVK VAVDVLKELEALMPSAAGQEKQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRS RTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGC FVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNR RRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFD EETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELK QAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILE QRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRV SEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGL LKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLT EPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATN IAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRD EMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRM AEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYN SWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDP QEGYRTLIDQQVVYIHPSSALFNRQPECPKHFLPVVAVAVSLEQCLSKFEDLNKELGLVTC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001302623
ORF Size	3543 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302623.2
RefSeq Size	3875 bp
RefSeq ORF	3546 bp
Locus ID	1659
UniProt ID	Q14562
Protein Pathways	Spliceosome
MW	134.5 kDa
Gene Summary	This gene is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

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