DHX8 (NM_001302623) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DDX8; Dhr2; HRH1; PRP22; PRPF22
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001302623, the custom clone sequence may differ by one or more nucleotides ATGGCTGTGGCTGTAGCCATGGCGGGAGCCTTAATCGGGTCGGAGCCAGGCCCCGCGGAAGAACTTGCCA AACTCGAGTACCTGTCTTTGGTGTCAAAGGTTTGCACTGAGCTGGACAATCACTTGGGGATCAACGACAA GGACCTTGCTGAATTTGTGATCAGTCTTGCTGAGAAAAATACCACCTTTGATACTTTTAAGGCTTCTCTC GTCAAAAATGGTGCAGAATTTACGGATTCTCTTATTAGTAACTTGCTGCGTCTCATACAAACCATGCGGC CTCCAGCGAAGCCTTCCACTAGCAAAGATCCAGTTGTTAAACCTAAAACAGAAAAAGAAAAGCTGAAGGA ACTCTTCCCAGTCCTTTGCCAACCGGACAACCCTTCTGTTCGGACCATGTTGGATGAAGATGATGTGAAA GTTGCTGTGGATGTCCTGAAAGAACTGGAAGCTTTAATGCCCAGCGCAGCAGGCCAGGAGAAGCAAAGAG ATGCTGAACACCGGGACAGGACAAAGAAGAAGAAGCGGAGTCGAAGCCGAGATCGAAACCGAGATCGAGA CAGAGATAGGGAACGAAACCGAGATAGAGACCACAAGCGGAGACACCGATCCCGCTCTCGATCACGTTCC AGGACCCGGGAGAGGAATAAAGTGAAGTCTAGATATCGGTCCAGGAGCAGGAGTCAGAGTCCCCCCAAAG ACCGGAAGGACCGGGACAAATATGGAGAGCGGAATCTGGATAGATGGCGGGATAAGCATGTGGACCGCCC TCCTCCAGAAGAGCCCACCATTGGTGACATTTATAATGGCAAAGTTACCAGCATCATGCAGTTTGGTTGC TTTGTGCAGCTGGAAGGACTAAGGAAGCGGTGGGAAGGCCTGGTGCACATCTCTGAGCTCCGGCGGGAGG GTCGTGTGGCCAATGTAGCTGATGTCGTGAGCAAAGGCCAGAGGGTCAAAGTCAAAGTGCTGTCCTTCAC TGGGACCAAGACCAGCCTGAGCATGAAGGATGTGGATCAAGAGACTGGAGAAGATCTAAACCCAAATAGA CGGCGAAATCTTGTCGGGGAGACCAATGAGGAGACCTCAATGCGGAATCCTGATAGACCCACTCACTTGT CCCTTGTCAGTGCTCCTGAAGTAGAGGACGACTCACTGGAACGCAAGCGCCTCACCCGAATCTCTGACCC AGAGAAGTGGGAGATCAAACAGATGATTGCTGCCAATGTCCTTTCCAAAGAAGAATTTCCAGACTTTGAT GAAGAGACTGGCATTCTCCCTAAGGTGGATGATGAAGAAGATGAGGACCTTGAGATTGAATTGGTTGAGG AAGAGCCTCCATTCCTGAGAGGGCACACTAAGCAAAGCATGGACATGAGCCCCATTAAAATTGTCAAGAA CCCAGACGGCTCCCTCTCCCAAGCAGCAATGATGCAGAGTGCCTTGGCCAAAGAAAGGCGGGAACTCAAA CAGGCCCAGCGGGAAGCTGAGATGGATTCTATTCCCATGGGACTCAACAAACACTGGGTTGACCCTCTGC CTGATGCGGAAGGCAGACAGATTGCTGCCAACATGAGGGGTATTGGGATGATGCCCAATGATATTCCTGA GTGGAAGAAGCATGCCTTTGGGGGCAACAAAGCCTCTTACGGAAAAAAGACCCAGATGTCAATCCTTGAG CAGAGGGAGAGCCTGCCCATCTACAAACTGAAGGAGCAATTGGTCCAGGCCGTCCATGACAATCAGATCC TGATTGTCATTGGTGAGACAGGATCTGGAAAGACAACACAGATCACCCAGTACCTGGCGGAGGCAGGCTA CACTTCCAGGGGCAAGATTGGGTGTACCCAGCCCAGAAGAGTGGCAGCTATGTCGGTGGCCAAAAGAGTG TCAGAGGAGTTTGGTTGTTGCTTAGGCCAAGAGGTGGGCTACACCATTCGATTTGAGGACTGCACTAGCC CTGAAACAGTCATCAAGTACATGACAGATGGGATGTTGCTTAGAGAGTGCTTGATTGACCCTGACCTCAC TCAGTACGCGATCATCATGTTGGACGAGGCACATGAGAGGACAATTCACACTGATGTGCTCTTTGGATTG TTGAAAAAGACAGTTCAGAAACGGCAGGACATGAAGCTGATTGTCACCTCAGCCACCTTGGATGCAGTGA AGTTTTCTCAATACTTCTATGAAGCTCCCATTTTCACCATCCCAGGTCGAACATATCCAGTGGAAATACT GTACACAAAGGAACCTGAGACAGATTATCTGGATGCCAGCCTGATTACTGTTATGCAGATTCATTTAACA GAACCACCAGGTGATATCCTGGTCTTCCTGACTGGTCAGGAAGAAATTGATACTGCTTGTGAGATCCTGT ATGAAAGAATGAAATCCCTGGGACCTGATGTTCCAGAGTTAATTATCCTCCCAGTGTACTCTGCTCTTCC CAGTGAGATGCAGACCCGAATCTTTGACCCAGCTCCACCAGGCAGCAGAAAGGTTGTGATTGCCACCAAT ATCGCAGAGACATCGCTGACTATTGATGGTATCTACTATGTGGTGGACCCAGGATTCGTGAAACAGAAAG TTTACAATTCCAAGACAGGGATTGACCAGCTCGTGGTGACGCCTATTTCTCAGGCTCAGGCAAAGCAACG AGCTGGCAGAGCTGGGAGAACAGGCCCAGGGAAGTGTTACAGGTTGTACACAGAACGTGCCTACCGAGAT GAAATGCTGACCACCAACGTGCCGGAAATCCAGAGAACCAACTTAGCAAGCACAGTGCTGTCACTCAAGG CCATGGGTATCAATGATCTGCTGTCCTTTGATTTCATGGATGCCCCACCTATGGAAACTTTGATCACAGC CATGGAGCAGCTGTACACACTGGGGGCCCTGGATGACGAGGGCCTGCTCACTCGCTTGGGCCGCCGGATG GCAGAGTTCCCTCTGGAGCCAATGCTATGCAAAATGCTCATCATGTCTGTGCATCTGGGCTGCAGTGAGG AAATGCTGACCATTGTATCCATGCTGTCTGTGCAGAACGTCTTCTATAGGCCCAAGGATAAACAAGCCCT TGCAGATCAGAAGAAGGCCAAATTCCACCAGACTGAAGGGGACCACCTCACCCTGCTAGCTGTGTACAAC TCCTGGAAGAACAACAAGTTCTCCAACCCATGGTGCTATGAGAACTTTATCCAGGCTCGTTCCCTGCGCC GGGCCCAGGACATTCGCAAGCAGATGTTAGGCATAATGGACAGACACAAGCTGGATGTTGTTTCCTGTGG CAAGTCCACAGTCCGAGTGCAGAAGGCCATCTGCAGTGGGTTCTTCCGTAATGCTGCCAAGAAAGACCCG CAGGAGGGTTACCGGACACTGATCGACCAGCAGGTGGTCTATATCCATCCTTCCAGTGCCCTCTTCAACA GACAGCCAGAATGCCCAAAGCACTTCCTCCCTGTGGTTGCTGTAGCTGTCTCATTGGAGCAGTGCCTCTC CAAGTTTGAGGATTTGAACAAAGAACTGGGACTGGTGACTTGTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302623
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302623.1, NP_001289552.1
RefSeq Size	3875 bp
RefSeq ORF	3546 bp
Locus ID	1659
UniProt ID	Q14562
Protein Pathways	Spliceosome
Gene Summary	This gene is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.