ZFYVE16 (NM_001284237) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PPP1R69
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001284237, the custom clone sequence may differ by one or more nucleotides ATGGACAGTTATTTTAAAGCAGCTGTCAGTGACTTGGACAAACTCCTTGATGATTTTGAACAGAACCCAG ATGAACAAGATTATCTCCAAGATGTACAAAATGCATATGATTCTAACCACTGCTCAGTTTCTTCAGAGTT GGCTTCCTCACAGCGAACTTCATTGCTCCCAAAAGACCAAGAGTGCGTTAATAGTTGTGCCTCATCAGAA ACAAGCTATGGAACAAATGAGAGTTCCCTGAATGAAAAAACACTCAAGGGACTTACTTCTATACAAAATG AAAAAAATGTAACAGGACTTGATCTTCTTTCTTCTGTGGATGGTGGTACTTCAGATGAAATCCAGCCGTT ATATATGGGACGATGTAGTAAACCTATCTGTGATCTGATAAGTGACATGGGTAACTTAGTTCATGCAACC AATAGTGAAGAAGATATTAAAAAATTATTGCCAGATGATTTTAAGTCTAATGCAGATTCCTTGATTGGAT TGGATTTATCTTCAGTGTCAGATACTCCCTGTGTTTCTTCAACAGACCATGATAGTGATACTGTCAGAGA ACAACAGAATGATATCAGTTCTGAATTACAAAATAGAGAAATCGGAGGAATCAAAGAATTGGGTATAAAA GTAGATACAACACTTTCAGATTCCTATAATTACAGTGGAACAGAAAATTTAAAAGATAAAAAGATCTTTA ATCAGTTAGAATCAATTGTTGATTTTAACATGTCATCTGCTTTGACTCGACAAAGTTCCAAAATGTTTCA TGCCAAAGACAAGCTACAACACAAGAGCCAGCCATGTGGATTACTAAAAGATGTTGGCTTAGTAAAAGAG GAAGTAGATGTGGCAGTCATAACTGCCGCAGAATGTTTAAAAGAAGAGGGCAAGACAAGTGCTTTGACCT GCAGCCTTCCGAAAAATGAAGATTTATGCTTAAATGATTCAAATTCAAGAGATGAAAATTTCAAATTACC TGACTTTTCCTTTCAGGAAGATAAGACTGTTATAAAACAATCTGCACAAGAAGACTCAAAAAGTTTAGAC CTTAAGGATAATGATGTAATCCAAGATTCCTCTTCAGCTTTACATGTTTCCAGTAAAGATGTGCCGTCCT CATTGTCCTGTCTTCCTGCGTCTGGGTCTATGTGTGGATCATTAATTGAAAGTAAAGCACGGGGTGATTT TTTACCTCAGCATGAACATAAAGATAATATACAAGATGCAGTGACTATACATGAAGAAATACAGAACAGT GTTGTTCTAGGTGGGGAACCATTCAAAGAGAATGATCTTTTGAAACAGGAAAAATGTAAAAGCATACTCC TTCAGTCATTAATTGAAGGGATGGAAGACAGAAAGATAGATCCTGACCAGACAGTAATCAGAGCTGAGTC TTTGGATGGTGGTGACACCAGTTCTACAGTTGTAGAATCTCAAGAGGGGCTTTCTGGCACTCATGTCCCA GAGTCTTCTGATTGTTGTGAAGGTTTTATTAATACTTTTTCAAGCAATGATATGGATGGGCAAGACTTAG ATTACTTTAATATTGATGAAGGCGCAAAAAGTGGCCCACTAATTAGTGATGCTGAACTTGATGCCTTTCT GACAGAACAGTATCTTCAGACCACTAACATAAAGTCTTTTGAAGAAAATGTAAATGACTCTAAATCGCAA ATGAATCAGATAGATATGAAAGGCTTAGATGATGGAAACATCAATAATATATATTTCAATGCAGAAGCAG GAGCTATTGGGGAAAGTCATGGTATTAATATAATTTGTGAAACAGTTGATAAACAAAATACAATAGAAAA TGGCCTTTCTTTAGGAGAAAAAAGCACTATTCCAGTTCAACAAGGGTTACCTACCAGTAAGTCTGAGATT ACAAATCAATTATCAGTCTCTGATATTAACAGTCAATCTGTTGGAGGGGCCAGACCTAAGCAATTGTTTA GCCTTCCATCAAGAACAAGGAGTTCAAAGGACCTGAATAAGCCAGATGTTCCAGATACAATAGAAAGTGA ACCCAGCACAGCAGATACCGTTGTTCCAATCACTTGTGCTATAGATTCTACAGCTGATCCACAGGTTAGC TTCAACTCTAATTACATTGATATAGAAAGTAATTCTGAAGGTGGATCTAGTTTCGTAACTGCAAATGAAG ATTCTGTACCTGAAAACACTTGCAAAGAAGGCTTGGTTTTGGGCCAGAAACAGCCTACTTGGGTTCCTGA TTCAGAAGCTCCAAACTGTATGAACTGCCAAGTCAAATTTACTTTTACCAAACGGCGACACCATTGCCGA GCATGTGGGAAAGTATTTTGTGGTGTCTGTTGTAATAGGAAGTGTAAACTGCAATATCTAGAAAAGGAAG CAAGAGTATGTGTAGTCTGCTATGAAACTATTAGTAAAGGTGAGTATTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001284237
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284237.1, NP_001271166.1
RefSeq Size	2972 bp
RefSeq ORF	2430 bp
Locus ID	9765
UniProt ID	Q7Z3T8
Protein Pathways	TGF-beta signaling pathway
Gene Summary	This gene encodes an endosomal protein that belongs to the FYVE zinc finger family of proteins. The encoded protein is thought to regulate membrane trafficking in the endosome. This protein functions as a scaffold protein in the transforming growth factor-beta signaling pathway and is involved in positive and negative feedback regulation of the bone morphogenetic protein signaling pathway. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (4) lacks several exons in the 3' coding region and contains a 3' terminal exon that extends past a splice site that is used in variant 1 resulting in an early stop codon, and a different 3' UTR, compared to variant 1. The encoded protein (isoform b) has a distinct C-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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