ZFYVE16 (NM_001284237) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PPP1R69
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239610 representing NM_001284237 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGTTATTTTAAAGCAGCTGTCAGTGACTTGGACAAACTCCTTGATGATTTTGAACAGAACCCAG ATGAACAAGATTATCTCCAAGATGTACAAAATGCATATGATTCTAACCACTGCTCAGTTTCTTCAGAGTT GGCTTCCTCACAGCGAACTTCATTGCTCCCAAAAGACCAAGAGTGCGTTAATAGTTGTGCCTCATCAGAA ACAAGCTATGGAACAAATGAGAGTTCCCTGAATGAAAAAACACTCAAGGGACTTACTTCTATACAAAATG AAAAAAATGTAACAGGACTTGATCTTCTTTCTTCTGTGGATGGTGGTACTTCAGATGAAATCCAGCCGTT ATATATGGGACGATGTAGTAAACCTATCTGTGATCTGATAAGTGACATGGGTAACTTAGTTCATGCAACC AATAGTGAAGAAGATATTAAAAAATTATTGCCAGATGATTTTAAGTCTAATGCAGATTCCTTGATTGGAT TGGATTTATCTTCAGTGTCAGATACTCCCTGTGTTTCTTCAACAGACCATGATAGTGATACTGTCAGAGA ACAACAGAATGATATCAGTTCTGAATTACAAAATAGAGAAATCGGAGGAATCAAAGAATTGGGTATAAAA GTAGATACAACACTTTCAGATTCCTATAATTACAGTGGAACAGAAAATTTAAAAGATAAAAAGATCTTTA ATCAGTTAGAATCAATTGTTGATTTTAACATGTCATCTGCTTTGACTCGACAAAGTTCCAAAATGTTTCA TGCCAAAGACAAGCTACAACACAAGAGCCAGCCATGTGGATTACTAAAAGATGTTGGCTTAGTAAAAGAG GAAGTAGATGTGGCAGTCATAACTGCCGCAGAATGTTTAAAAGAAGAGGGCAAGACAAGTGCTTTGACCT GCAGCCTTCCGAAAAATGAAGATTTATGCTTAAATGATTCAAATTCAAGAGATGAAAATTTCAAATTACC TGACTTTTCCTTTCAGGAAGATAAGACTGTTATAAAACAATCTGCACAAGAAGACTCAAAAAGTTTAGAC CTTAAGGATAATGATGTAATCCAAGATTCCTCTTCAGCTTTACATGTTTCCAGTAAAGATGTGCCGTCCT CATTGTCCTGTCTTCCTGCGTCTGGGTCTATGTGTGGATCATTAATTGAAAGTAAAGCACGGGGTGATTT TTTACCTCAGCATGAACATAAAGATAATATACAAGATGCAGTGACTATACATGAAGAAATACAGAACAGT GTTGTTCTAGGTGGGGAACCATTCAAAGAGAATGATCTTTTGAAACAGGAAAAATGTAAAAGCATACTCC TTCAGTCATTAATTGAAGGGATGGAAGACAGAAAGATAGATCCTGACCAGACAGTAATCAGAGCTGAGTC TTTGGATGGTGGTGACACCAGTTCTACAGTTGTAGAATCTCAAGAGGGGCTTTCTGGCACTCATGTCCCA GAGTCTTCTGATTGTTGTGAAGGTTTTATTAATACTTTTTCAAGCAATGATATGGATGGGCAAGACTTAG ATTACTTTAATATTGATGAAGGCGCAAAAAGTGGCCCACTAATTAGTGATGCTGAACTTGATGCCTTTCT GACAGAACAGTATCTTCAGACCACTAACATAAAGTCTTTTGAAGAAAATGTAAATGACTCTAAATCGCAA ATGAATCAGATAGATATGAAAGGCTTAGATGATGGAAACATCAATAATATATATTTCAATGCAGAAGCAG GAGCTATTGGGGAAAGTCATGGTATTAATATAATTTGTGAAACAGTTGATAAACAAAATACAATAGAAAA TGGCCTTTCTTTAGGAGAAAAAAGCACTATTCCAGTTCAACAAGGGTTACCTACCAGTAAGTCTGAGATT ACAAATCAATTATCAGTCTCTGATATTAACAGTCAATCTGTTGGAGGGGCCAGACCTAAGCAATTGTTTA GCCTTCCATCAAGAACAAGGAGTTCAAAGGACCTGAATAAGCCAGATGTTCCAGATACAATAGAAAGTGA ACCCAGCACAGCAGATACCGTTGTTCCAATCACTTGTGCTATAGATTCTACAGCTGATCCACAGGTTAGC TTCAACTCTAATTACATTGATATAGAAAGTAATTCTGAAGGTGGATCTAGTTTCGTAACTGCAAATGAAG ATTCTGTACCTGAAAACACTTGCAAAGAAGGCTTGGTTTTGGGCCAGAAACAGCCTACTTGGGTTCCTGA TTCAGAAGCTCCAAACTGTATGAACTGCCAAGTCAAATTTACTTTTACCAAACGGCGACACCATTGCCGA GCATGTGGGAAAGTATTTTGTGGTGTCTGTTGTAATAGGAAGTGTAAACTGCAATATCTAGAAAAGGAAG CAAGAGTATGTGTAGTCTGCTATGAAACTATTAGTAAAGGTGAGTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239610 representing NM_001284237 Red=Cloning site Green=Tags(s) MDSYFKAAVSDLDKLLDDFEQNPDEQDYLQDVQNAYDSNHCSVSSELASSQRTSLLPKDQECVNSCASSE TSYGTNESSLNEKTLKGLTSIQNEKNVTGLDLLSSVDGGTSDEIQPLYMGRCSKPICDLISDMGNLVHAT NSEEDIKKLLPDDFKSNADSLIGLDLSSVSDTPCVSSTDHDSDTVREQQNDISSELQNREIGGIKELGIK VDTTLSDSYNYSGTENLKDKKIFNQLESIVDFNMSSALTRQSSKMFHAKDKLQHKSQPCGLLKDVGLVKE EVDVAVITAAECLKEEGKTSALTCSLPKNEDLCLNDSNSRDENFKLPDFSFQEDKTVIKQSAQEDSKSLD LKDNDVIQDSSSALHVSSKDVPSSLSCLPASGSMCGSLIESKARGDFLPQHEHKDNIQDAVTIHEEIQNS VVLGGEPFKENDLLKQEKCKSILLQSLIEGMEDRKIDPDQTVIRAESLDGGDTSSTVVESQEGLSGTHVP ESSDCCEGFINTFSSNDMDGQDLDYFNIDEGAKSGPLISDAELDAFLTEQYLQTTNIKSFEENVNDSKSQ MNQIDMKGLDDGNINNIYFNAEAGAIGESHGINIICETVDKQNTIENGLSLGEKSTIPVQQGLPTSKSEI TNQLSVSDINSQSVGGARPKQLFSLPSRTRSSKDLNKPDVPDTIESEPSTADTVVPITCAIDSTADPQVS FNSNYIDIESNSEGGSSFVTANEDSVPENTCKEGLVLGQKQPTWVPDSEAPNCMNCQVKFTFTKRRHHCR ACGKVFCGVCCNRKCKLQYLEKEARVCVVCYETISKGEY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001284237
ORF Size	2427 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284237.1, NP_001271166.1
RefSeq Size	2972 bp
RefSeq ORF	2430 bp
Locus ID	9765
UniProt ID	Q7Z3T8
Protein Pathways	TGF-beta signaling pathway
MW	88.9 kDa
Gene Summary	This gene encodes an endosomal protein that belongs to the FYVE zinc finger family of proteins. The encoded protein is thought to regulate membrane trafficking in the endosome. This protein functions as a scaffold protein in the transforming growth factor-beta signaling pathway and is involved in positive and negative feedback regulation of the bone morphogenetic protein signaling pathway. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

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