Zonula occludens protein 3 (TJP3) (NM_001267561) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ZO-3; ZO3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332853 representing NM_001267561. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGGTGAGATTCCAGGTGGCTGACATGGAGGAGCTGACCATCTGGGAACAGCACACGGCCACACTG TCCAAGGACCCCCGCCGGGGCTTTGGCATTGCGATCTCTGGAGGCCGAGACCGGCCCGGTGGATCCATG GTTGTATCTGACGTGGTACCTGGAGGGCCGGCGGAGGGCAGGCTACAGACAGGCGACCACATCGTCATG GTGAACGGGGTTTCCATGGAGAATGCCACCTCCGCGTTTGCCATTCAGATACTCAAGACCTGCACCAAG ATGGCCAACATCACAGTGAAACGTCCCCGGAGGATCCACCTGCCCGCCACCAAAGCCAGCCCCTCCAGC CCAGGGCGCCAGGACTCGGATGAAGACGATGGGCCCCAGCGGGTGGAGGAGGTGGACCAGGGCCGGGGC TATGACGGCGACTCATCCAGTGGCTCCGGCCGCTCCTGGGACGAGCGCTCCCGCCGGCCGAGGCCTGGT CGCCGGGGCCGGGCCGGCAGCCATGGGCGTAGGAGCCCAGGTGGTGGCTCTGAGGCCAACGGGCTGGCC CTGGTGTCCGGCTTTAAGCGGCTGCCACGGCAGGACGTGCAGATGAAGCCTGTGAAGTCAGTGCTGGTG AAGAGGAGAGACAGCGAAGAGTTTGGCGTCAAGCTGGGCAGTCAGATCTTCATCAAGCACATTACAGAT TCGGGCCTGGCTGCCCGGCACCGTGGGCTGCAGGAAGGAGATCTCATTCTACAGATCAACGGGGTGTCT AGCCAGAACCTGTCACTGAACGACACCCGGCGACTGATTGAGAAGTCAGAAGGGAAGCTAAGCCTGCTG GTGCTGAGAGATCGTGGGCAGTTCCTGGTGAACATTCCGCCTGCTGTCAGTGACAGCGACAGCTCGCCA TTGGAGGACATCTCGGACCTCGCCTCGGAGCTATCGCAGGCACCACCATCCCACATCCCACCACCACCC CGGCATGCTCAGCGGAGCCCCGAGGCCAGCCAGACCGACTCTCCCGTGGAGAGTCCCCGGCTTCGGCGG GAAAGTTCAGTAGATTCCAGAACCATCTCGGAACCAGATGAGCAACGGTCAGAGTTGCCCAGGGAAAGC AGCTATGACATCTACAGAGTGCCCAGCAGTCAGAGCATGGAGGATCGTGGGTACAGCCCCGACACGCGT GTGGTCCGCTTCCTCAAGGGCAAGAGCATCGGGCTGCGGCTGGCAGGGGGCAATGACGTGGGCATCTTC GTGTCCGGGGTGCAGGCGGGCAGCCCGGCCGACGGGCAGGGCATCCAGGAGGGAGATCAGATTCTGCAG GTGAATGACGTGCCATTCCAGAACCTGACACGGGAGGAGGCAGTGCAGTTCCTGCTGGGGCTGCCACCA GGCGAGGAGATGGAGCTGGTGACGCAGAGGAAGCAGGACATTTTCTGGAAAATGGTGCAGTCCCGCGTG GGTGACTCCTTCTACATCCGCACTCACTTTGAGCTGGAGCCCAGTCCACCGTCTGGCCTGGGCTTCACC CGTGGCGACGTCTTCCACGTGCTGGACACGCTGCACCCCGGCCCCGGGCAGAGCCACGCACGAGGAGGC CACTGGCTGGCGGTGCGCATGGGTCGTGACCTGCGGGAGCAAGAGCGGGGCATCATTCCCAACCAGAGC AGGGCGGAGCAGCTGGCCAGCCTGGAAGCTGCCCAGAGGGCCGTGGGAGTCGGGCCCGGCTCCTCCGCG GGCTCCAATGCTCGGGCCGAGTTCTGGCGGCTGCGGGGTCTTCGTCGAGGAGCCAAGAAGACCACTCAG CGGAGCCGTGAGGACCTCTCAGCTCTGACCCGACAGGGCCGCTACCCGCCCTACGAACGAGTGGTGTTG CGAGAAGCCAGTTTCAAGCGCCCGGTAGTGATCCTGGGACCCGTGGCCGACATTGCTATGCAGAAGTTG ACTGCTGAGATGCCTGACCAGTTTGAAATCGCAGAGACTGTGTCCAGGACCGACAGCCCCTCCAAGATC ATCAAACTAGACACCGTGCGGGTGATTGCAGAAAAAGACAAGCATGCGCTCCTGGATGTGACCCCCTCC GCCATCGAGCGCCTCAACTATGTGCAGTACTACCCCATTGTGGTCTTCTTCATCCCCGAGAGCCGGCCG GCCCTCAAGGCACTGCGCCAGTGGCTGGCGCCTGCCTCCCGCCGCAGCACCCGTCGCCTCTACGCACAA GCCCAGAAGCTGCGAAAACACAGCAGCCACCTCTTCACAGCCACCATCCCTCTGAATGGCACGAGTGAC ACCTGGTACCAGGAGCTCAAGGCCATCATTCGAGAGCAGCAGACGCGGCCCATCTGGACGGCGGAAGAT CAGCTGGATGGCTCCTTGGAGGACAACCTAGACCTCCCTCACCACGGCCTGGCCGACAGCTCCGCTGAC CTCAGCTGCGACAGCCGCGTTAACAGCGACTACGAGACGGACGGCGAGGGCGGCGCGTACACGGATGGC GAGGGCTACACAGACGGCGAGGGGGGGCCCTACACGGATGTGGATGATGAGCCCCCGGCTCCAGCCCTG GCCCGGTCCTCGGAGCCCGTGCAGGCAGATGAGTCCCAGAGCCCGAGGGATCGTGGGAGAATCTCGGCT CATCAGGGGGCCCAGGTGGACAGCCGCCACCCCCAGGGACAGTGGCGACAGGACAGCATGCGAACCTAT GAACGGGAAGCCCTGAAGAAAAAGTTTATGCGAGTACATGATGCGGAGTCCTCCGATGAAGACGGCTAT GACTGGGGTCCGGCCACTGACCTGTGA
Restriction Sites	SgfI-RsrII
ACCN	NM_001267561
Insert Size	2787 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001267561.1
RefSeq Size	3127 bp
RefSeq ORF	2787 bp
Locus ID	27134
UniProt ID	O95049
Protein Pathways	Tight junction
MW	102.4 kDa
Gene Summary	The protein encoded by this gene is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by this gene plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This gene has a partial pseudogene on chromosome 1. [provided by RefSeq, May 2012] Transcript Variant: This variant (2) differs in the 5' UTR and represents use of an alternate promoter, compared to variant 1, which results in a protein (isoform 2) with a longer and distinct N-terminus, compared to isoform 1.

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