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Zonula occludens protein 3 (TJP3) (NM_001267561) Human Tagged ORF Clone
CAT＃: RG235043
- TrueORF®
TJP3 (tGFP-tagged) - Homo sapiens tight junction protein 3 (TJP3), transcript variant 2
ORF Plasmid: DDK
"NM_001267561" in other vectors (2)
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CNY 10,170.00
货期*
5周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ZO-3; ZO3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235043 representing NM_001267561
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTGAGATTCCAGGTGGCTGACATGGAGGAGCTGACCATCTGGGAACAGCACACGGCCACACTGT CCAAGGACCCCCGCCGGGGCTTTGGCATTGCGATCTCTGGAGGCCGAGACCGGCCCGGTGGATCCATGGT TGTATCTGACGTGGTACCTGGAGGGCCGGCGGAGGGCAGGCTACAGACAGGCGACCACATCGTCATGGTG AACGGGGTTTCCATGGAGAATGCCACCTCCGCGTTTGCCATTCAGATACTCAAGACCTGCACCAAGATGG CCAACATCACAGTGAAACGTCCCCGGAGGATCCACCTGCCCGCCACCAAAGCCAGCCCCTCCAGCCCAGG GCGCCAGGACTCGGATGAAGACGATGGGCCCCAGCGGGTGGAGGAGGTGGACCAGGGCCGGGGCTATGAC GGCGACTCATCCAGTGGCTCCGGCCGCTCCTGGGACGAGCGCTCCCGCCGGCCGAGGCCTGGTCGCCGGG GCCGGGCCGGCAGCCATGGGCGTAGGAGCCCAGGTGGTGGCTCTGAGGCCAACGGGCTGGCCCTGGTGTC CGGCTTTAAGCGGCTGCCACGGCAGGACGTGCAGATGAAGCCTGTGAAGTCAGTGCTGGTGAAGAGGAGA GACAGCGAAGAGTTTGGCGTCAAGCTGGGCAGTCAGATCTTCATCAAGCACATTACAGATTCGGGCCTGG CTGCCCGGCACCGTGGGCTGCAGGAAGGAGATCTCATTCTACAGATCAACGGGGTGTCTAGCCAGAACCT GTCACTGAACGACACCCGGCGACTGATTGAGAAGTCAGAAGGGAAGCTAAGCCTGCTGGTGCTGAGAGAT CGTGGGCAGTTCCTGGTGAACATTCCGCCTGCTGTCAGTGACAGCGACAGCTCGCCATTGGAGGACATCT CGGACCTCGCCTCGGAGCTATCGCAGGCACCACCATCCCACATCCCACCACCACCCCGGCATGCTCAGCG GAGCCCCGAGGCCAGCCAGACCGACTCTCCCGTGGAGAGTCCCCGGCTTCGGCGGGAAAGTTCAGTAGAT TCCAGAACCATCTCGGAACCAGATGAGCAACGGTCAGAGTTGCCCAGGGAAAGCAGCTATGACATCTACA GAGTGCCCAGCAGTCAGAGCATGGAGGATCGTGGGTACAGCCCCGACACGCGTGTGGTCCGCTTCCTCAA GGGCAAGAGCATCGGGCTGCGGCTGGCAGGGGGCAATGACGTGGGCATCTTCGTGTCCGGGGTGCAGGCG GGCAGCCCGGCCGACGGGCAGGGCATCCAGGAGGGAGATCAGATTCTGCAGGTGAATGACGTGCCATTCC AGAACCTGACACGGGAGGAGGCAGTGCAGTTCCTGCTGGGGCTGCCACCAGGCGAGGAGATGGAGCTGGT GACGCAGAGGAAGCAGGACATTTTCTGGAAAATGGTGCAGTCCCGCGTGGGTGACTCCTTCTACATCCGC ACTCACTTTGAGCTGGAGCCCAGTCCACCGTCTGGCCTGGGCTTCACCCGTGGCGACGTCTTCCACGTGC TGGACACGCTGCACCCCGGCCCCGGGCAGAGCCACGCACGAGGAGGCCACTGGCTGGCGGTGCGCATGGG TCGTGACCTGCGGGAGCAAGAGCGGGGCATCATTCCCAACCAGAGCAGGGCGGAGCAGCTGGCCAGCCTG GAAGCTGCCCAGAGGGCCGTGGGAGTCGGGCCCGGCTCCTCCGCGGGCTCCAATGCTCGGGCCGAGTTCT GGCGGCTGCGGGGTCTTCGTCGAGGAGCCAAGAAGACCACTCAGCGGAGCCGTGAGGACCTCTCAGCTCT GACCCGACAGGGCCGCTACCCGCCCTACGAACGAGTGGTGTTGCGAGAAGCCAGTTTCAAGCGCCCGGTA GTGATCCTGGGACCCGTGGCCGACATTGCTATGCAGAAGTTGACTGCTGAGATGCCTGACCAGTTTGAAA TCGCAGAGACTGTGTCCAGGACCGACAGCCCCTCCAAGATCATCAAACTAGACACCGTGCGGGTGATTGC AGAAAAAGACAAGCATGCGCTCCTGGATGTGACCCCCTCCGCCATCGAGCGCCTCAACTATGTGCAGTAC TACCCCATTGTGGTCTTCTTCATCCCCGAGAGCCGGCCGGCCCTCAAGGCACTGCGCCAGTGGCTGGCGC CTGCCTCCCGCCGCAGCACCCGTCGCCTCTACGCACAAGCCCAGAAGCTGCGAAAACACAGCAGCCACCT CTTCACAGCCACCATCCCTCTGAATGGCACGAGTGACACCTGGTACCAGGAGCTCAAGGCCATCATTCGA GAGCAGCAGACGCGGCCCATCTGGACGGCGGAAGATCAGCTGGATGGCTCCTTGGAGGACAACCTAGACC TCCCTCACCACGGCCTGGCCGACAGCTCCGCTGACCTCAGCTGCGACAGCCGCGTTAACAGCGACTACGA GACGGACGGCGAGGGCGGCGCGTACACGGATGGCGAGGGCTACACAGACGGCGAGGGGGGGCCCTACACG GATGTGGATGATGAGCCCCCGGCTCCAGCCCTGGCCCGGTCCTCGGAGCCCGTGCAGGCAGATGAGTCCC AGAGCCCGAGGGATCGTGGGAGAATCTCGGCTCATCAGGGGGCCCAGGTGGACAGCCGCCACCCCCAGGG ACAGTGGCGACAGGACAGCATGCGAACCTATGAACGGGAAGCCCTGAAGAAAAAGTTTATGCGAGTACAT GATGCGGAGTCCTCCGATGAAGACGGCTATGACTGGGGTCCGGCCACTGACCTG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235043 representing NM_001267561
Red=Cloning site Green=Tags(s) MAVRFQVADMEELTIWEQHTATLSKDPRRGFGIAISGGRDRPGGSMVVSDVVPGGPAEGRLQTGDHIVMV NGVSMENATSAFAIQILKTCTKMANITVKRPRRIHLPATKASPSSPGRQDSDEDDGPQRVEEVDQGRGYD GDSSSGSGRSWDERSRRPRPGRRGRAGSHGRRSPGGGSEANGLALVSGFKRLPRQDVQMKPVKSVLVKRR DSEEFGVKLGSQIFIKHITDSGLAARHRGLQEGDLILQINGVSSQNLSLNDTRRLIEKSEGKLSLLVLRD RGQFLVNIPPAVSDSDSSPLEDISDLASELSQAPPSHIPPPPRHAQRSPEASQTDSPVESPRLRRESSVD SRTISEPDEQRSELPRESSYDIYRVPSSQSMEDRGYSPDTRVVRFLKGKSIGLRLAGGNDVGIFVSGVQA GSPADGQGIQEGDQILQVNDVPFQNLTREEAVQFLLGLPPGEEMELVTQRKQDIFWKMVQSRVGDSFYIR THFELEPSPPSGLGFTRGDVFHVLDTLHPGPGQSHARGGHWLAVRMGRDLREQERGIIPNQSRAEQLASL EAAQRAVGVGPGSSAGSNARAEFWRLRGLRRGAKKTTQRSREDLSALTRQGRYPPYERVVLREASFKRPV VILGPVADIAMQKLTAEMPDQFEIAETVSRTDSPSKIIKLDTVRVIAEKDKHALLDVTPSAIERLNYVQY YPIVVFFIPESRPALKALRQWLAPASRRSTRRLYAQAQKLRKHSSHLFTATIPLNGTSDTWYQELKAIIR EQQTRPIWTAEDQLDGSLEDNLDLPHHGLADSSADLSCDSRVNSDYETDGEGGAYTDGEGYTDGEGGPYT DVDDEPPAPALARSSEPVQADESQSPRDRGRISAHQGAQVDSRHPQGQWRQDSMRTYEREALKKKFMRVH DAESSDEDGYDWGPATDL SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001267561 |
| ORF Size | 2784 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001267561.2 |
| RefSeq Size | 3127 bp |
| RefSeq ORF | 2787 bp |
| Locus ID | 27134 |
| UniProt ID | O95049 |
| Protein Pathways | Tight junction |
| Gene Summary | The protein encoded by this gene is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by this gene plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This gene has a partial pseudogene on chromosome 1. [provided by RefSeq, May 2012] |
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