Secondary Antibodies

Bovine IgG (light chain) mouse monoclonal antibody, clone IVA285-1, Purified

Applications Western Blotting (Reducing Conditions): 0.5-1 μg/ml, overnight in 4°C.
SDS-PAGE (12% separating gel).
Positive Control and Sample Peparation: BS (Bovine Serum), dilution 1/50 in Laemmli reducing buffer, boiled in water bath for 3 min.
Strongly reacts with Bovine IgG light chains, weakly reacts with IgM.
Flow Cytometry.
Immunohistochemistry on Frozen Sections.
Reactivities Bovine, Sheep
Conjugation Unconjugated

Bovine IgG2 sheep polyclonal antibody, FITC

Applications Flow Cytometry: 1/20 - 1/100.
Immunohistochemistry on frozen sections: 1/20 - 1/100.
Reactivities Bovine
Conjugation FITC

Bovine IgG sheep polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on Frozen Sections: 1/200-1/2,000.
Reactivities Bovine
Conjugation FITC

Bovine IgG1 sheep polyclonal antibody, FITC

Applications Flow Cytometry: 1/20 - 1/100.
Immunohistochemistry on frozen sections: 1/20 - 1/100.
Reactivities Bovine
Conjugation FITC

Bovine IgM sheep polyclonal antibody, FITC

Applications Flow Cytometry: 1/20-1/100.
Immunohistochemistry on frozen sections: 1/200-1/2000.
Reactivities Bovine
Conjugation FITC

Bovine IgG (H+L chain) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Fow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Bovine
Conjugation Texas Red