Human IgM (Fc-region) mouse monoclonal antibody, clone CH2, Purified
| Applications | Western blot. Recommended dilution: 1 μg/ml. Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular and intracellular staining. Immunocytochemistry. ELISA. |
| Reactivities | Human |
| Conjugation | Unconjugated |
FITC Conjugated Goat Anti-Mouse IgG
| Applications | FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64 |
| Reactivities | Mouse IgG |
| Conjugation | FITC |
Mouse IgA (alpha chain specific) goat polyclonal antibody, Biotin
| Applications | ELISA: 1/5,000-1/20,000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, PE
| Applications | Flow Cytometry: (< / = 0.1 µg/10e6 cells) and Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells. FLISA: < / = 1.0 µg/ml |
| Reactivities | Rat |
| Conjugation | PE |
IGHG4 mouse monoclonal antibody, clone HP6025, AP, Purified
| Applications | ELISA: 1/1000-1/2000 (2-9). Immunohistochemistry on Frozen Sections: reported in literature (5,7,10,11). Immunohistochemistry on Paraffin Sections: reported in literature (10). Flow Cytometry: reported in literature (15, 18, 19). Immunocytochemistry: reported in literature (12). Western Blot: reported in literature (3,13-16). |
| Reactivities | Human |
| Conjugation | AP |
Bovine IgG (light chain) mouse monoclonal antibody, clone IVA285-1, Purified
| Applications | Western Blotting (Reducing Conditions): 0.5-1 μg/ml, overnight in 4°C. SDS-PAGE (12% separating gel). Positive Control and Sample Peparation: BS (Bovine Serum), dilution 1/50 in Laemmli reducing buffer, boiled in water bath for 3 min. Strongly reacts with Bovine IgG light chains, weakly reacts with IgM. Flow Cytometry. Immunohistochemistry on Frozen Sections. |
| Reactivities | Bovine, Sheep |
| Conjugation | Unconjugated |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, PE
| Applications | FLISA: ? 0.1 µg/ml Flow Cytometry: ? 0.1 µg/106 cells. |
| Reactivities | Mouse |
| Conjugation | PE |
Mouse IgG1 goat polyclonal antibody, FITC
| Applications | Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 106 cells in 100 µl. Immunohistochemistry on Frozen Sections. Immunocytochemistry: neat-1/10. |
| Reactivities | Mouse |
| Conjugation | FITC |
Mouse IgA (alpha chain specific) goat polyclonal antibody, FITC
| Applications | FLISA: 1/100–1/400. Flow Cytometry: ≤ 1 μg/106 cells. Immunofluorescence: ≤ 1 μg/106 cells. Immunohistochemistry. |
| Reactivities | Mouse |
| Conjugation | FITC |
