Securin (PTTG1) (NM_004219) Human Mass Spec Standard

Specifications

Product Data
Description	PTTG1 MS Standard C13 and N15-labeled recombinant protein (NP_004210)
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	RC211038
Predicted MW	22 kDa
Protein Sequence	Sequence Link (showhide) >RC211038 protein sequence Red=Cloning site Green=Tags(s) MATLIYVDKENGEPGTRVVAKDGLKLGSGPSIKALDGRSQVSTPRFGKTFDAPPALPKATRKALGTVNRA TEKSVKTKGPLKQKQPSFSAKKMTEKTVKAKSSVPASDDAYPEIEKFFPFNPLDFESFDLPEEHQIAHLP LSGVPLMILDEERELEKLFQLGPPSPVKMPSPPWESNLLQSPSSILSTLDVELPPVCCDIDI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-Myc/DDK
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration	>0.05 µg/µL as determined by microplate BCA method
Labeling Method	Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3
Reference Data
RefSeq	NP_004210
RefSeq Size	786
RefSeq ORF	606
Synonyms	EAP1; HPTTG; PTTG; TUTR1
Locus ID	9232
Cytogenetics	5q33.3
Summary	The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Cell cycle, Oocyte meiosis

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