BGRF1/BDRF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101061

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BGRF1/BDRF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129485



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CNY 10,450.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101061 represents NCBI reference of YP_001129485 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTATGCATCTCAGAGGGGTAGGCTTACTGAAAATCTGCGGAATGCCCTCCAGCAGGATTCTACTA
CACAGGGCTGCCTGGGTGCCGAAACTCCATCCATCATGTATACTGGAGCAAAATCTGACCGCTGGGCACA
TCCTCTGGTGGGAACCATTCATGCCTCTAATTTGTACTGTCCAATGCTGAGAGCCTATTGCCGACACTAC
GGTCCCAGGCCGGTGTTTGTAGCCTCCGACGAGTCTCTGCCTATGTTCGGCGCCTCCCCCGCACTCCACA
CACCTGTCCAGGTACAGATGTGCCTCCTGCCAGAGCTTAGGGACACACTCCAGAGGCTCCTCCCGCCTCC
CAACCTGGAAGATTCTGAGGCTCTCACTGAGTTCAAAACAAGTGTCTCCAGTGCCCGGGCGATACTGGAG
GACCCTAATTTCCTCGAAATGAGGGAGTTTGTCACCTCACTCGCCAGCTTTCTGAGTGGGCAGTATAAAC
ACAAACCCGCTCGGCTGGAAGCGTTCCAGAAACAAGTGGTCCTGCACAGCTTTTATTTCCTGATAAGCAT
TAAATCACTGGAGATTACCGATACCATGTTTGATATTTTCCAAAGCGCATTTGGCCTGGAAGAAATGACA
CTTGAGAAACTCCATATTTTTAAACAGAAGGCGAGCGTGTTTCTTATTCCTCGGCGACATGGCAAGACAT
GGATCGTTGTAGCCATCATTTCCCTGATTCTTTCCAACCTTTCAAATGTCCAGATCGGATACGTTGCACA
CCAGAAACATGTTGCTTCCGCTGTGTTCACAGAGATCATTGATACCCTGACCAAGAGCTTTGACAGTAAG
AGAGTGGAGGTTAATAAAGAAACTAGTACCATCACCTTTAGGCACTCAGGGAAAATCAGCTCTACGGTTA
TGTGCGCAACATGTTTCAATAAGAACTCTATTCGCGGGCAGACCTTTCACTTGCTTTTCGTTGACGAGGC
CAATTTTATAAAAAAAGAGGCCCTGCCAGCCATCCTTGGATTCATGTTGCAGAAAGACGCCAAGATCATC
TTCATTTCTTCAGTAAATTCCGCGGACCAAGCAACTTCTTTCCTGTACAAGCTGAAGGACGCCCAAGAAC
GGCTCCTGAACGTCGTGTCATATGTATGTCAGGAACACCGGCAAGATTTCGACATGCAAGACAGTATGGT
GTCCTGTCCATGCTTTCGGCTCCATATTCCATCTTACATAACCATGGATAGCAACATTCGAGCCACAACG
AACCTCTTTCTTGACGGAGCTTTCAGCACAGAACTGATGGGCGATACCTCTAGTTTGAGTCAAGGATCAC
TTTCACGAACCGTCCGGGATGATGCCATAAATCAACTCGAATTGTGCAGGGTGGACACTCTGAATCCTCG
GGTTGCAGGAAGACTGGCTTCATCACTCTACGTGTACGTTGACCCCGCATATACAAATAACACATCAGCT
TCCGGAACGGGGATCGCAGCAGTTACTCATGATCGGGCCGACCCTAATCGCGTGATCGTTCTTGGGCTGG
AACATTTTTTTCTGAAGGATCTGACTGGTGATGCCGCCCTGCAGATAGCTACCTGCGTGGTGGCCCTTGT
GAGTTCTATTGTAACCCTGCACCCGCACCTGGAAGAGGTGAAAGTTGCAGTGGAAGGTAACTCTAGCCAA
GATAGCGCTGTAGCTATCGCTTCAATTATCGGCGAATCATGCCCTCTGCCTTGTGCCTTCGTCCATACAA
AGGATAAAACATCAAGTCTGCAGTGGCCTATGTATCTGTTGACTAATGAGAAGTCTAAAGCTTTCGAAAG
GCTCATTTATGCCGTTAACACCGCAAGTCTCTCTGCTAGTCAGGTTACCGTTTCCAACACGATTCAACTT
AGTTTCGACCCTGTGCTGTACCTGATCAGCCAGATTAGAGCCATCAAGCCCATTCCGCTGCGGGACGGTA
CTTACACCTACACCGGGAAACAAAGAAATCTGTCTGATGACGTTCTCGTCGCCCTCGTGATGGCCCACTT
CCTCGCCACAACCCAGAAACATACCTTCAAAAAAGTGCAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101061 representing YP_001129485
Red=Cloning sites Green=Tags

MLYASQRGRLTENLRNALQQDSTTQGCLGAETPSIMYTGAKSDRWAHPLVGTIHASNLYCPMLRAYCRHY
GPRPVFVASDESLPMFGASPALHTPVQVQMCLLPELRDTLQRLLPPPNLEDSEALTEFKTSVSSARAILE
DPNFLEMREFVTSLASFLSGQYKHKPARLEAFQKQVVLHSFYFLISIKSLEITDTMFDIFQSAFGLEEMT
LEKLHIFKQKASVFLIPRRHGKTWIVVAIISLILSNLSNVQIGYVAHQKHVASAVFTEIIDTLTKSFDSK
RVEVNKETSTITFRHSGKISSTVMCATCFNKNSIRGQTFHLLFVDEANFIKKEALPAILGFMLQKDAKII
FISSVNSADQATSFLYKLKDAQERLLNVVSYVCQEHRQDFDMQDSMVSCPCFRLHIPSYITMDSNIRATT
NLFLDGAFSTELMGDTSSLSQGSLSRTVRDDAINQLELCRVDTLNPRVAGRLASSLYVYVDPAYTNNTSA
SGTGIAAVTHDRADPNRVIVLGLEHFFLKDLTGDAALQIATCVVALVSSIVTLHPHLEEVKVAVEGNSSQ
DSAVAIASIIGESCPLPCAFVHTKDKTSSLQWPMYLLTNEKSKAFERLIYAVNTASLSASQVTVSNTIQL
SFDPVLYLISQIRAIKPIPLRDGTYTYTGKQRNLSDDVLVALVMAHFLATTQKHTFKKVH

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_009334
ORF Size 2070 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_009334.1, YP_001129485
RefSeq ORF 2070 bp
Locus ID 5176176
MW 76.7 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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