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BBRF2 (NC_009334) Virus Tagged ORF Clone
CAT＃: VC101054
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for BBRF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129477
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CNY 3,800.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC101054 represents NCBI reference of YP_001129477 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGTGGCAAGCACCATCAGCCCGGAGGCACAAGATCCCTTACCATGCAGAAAGTATCCCTGCGCG TGACCCCTCGGCTGGTGCTGGAGGTGAATAGACACAATGCAATTTGCGTGGCCACTAATGTGCCTGAGTT TTACAATGCTAGAGGGGATCTTAATATCCGGGATCTTCGGGCACACGTCAAGGCCAGGATGATCTCCAGT CAATTTTGCGGATACGTGCTGGTGTCACTGCTTGACTCTGAGGATCAGGTCGATCATCTGAACATTTTCC CTCACGTGTTCTCCGAGCGGATGATCTTGTATAAGCCTAATAACGTGAATCTCATGGAGATGTGCGCCCT GCTGTCTATGATTGAAAACGCCAAGTCCCCATCAATCGGACTGTGCCGGGAGGTACTCGGCCGCTTGACC TTGCTGCACTCTAAATGCAATAACCTGGACAGTCTGTTCCTTTACAATGGTGCCCGCACTCTTCTTTCTA CACTGGTCAAATACCATGATCTGGAGGAGGGAGCAGCCACTCCTGGTCCGTGGAATGAAGGTCTCAGCCT TTTTAAACTGCACAAAGAACTTAAGCGCGCACCAAGTGAGGCGCGCGATTTGATGCAGAGTTTGTTCCTG ACCAGCGGCAAGATGGGGTGCCTGGCCAGGAGCCCTAAGGACTATTGTGCCGATCTCAACAAGGAAGAGG ATGCGAACAGCGGATTCACGTTCAATCTGTTTTACCAAGATAGTCTGCTTACCAAGCATTTCCAGTGTCA GACTGTGTTGCAGACACTGAGAAGGAAGTGCTTGGGAAGCGACACTGTGAGCAAGATAATTCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101054 representing YP_001129477
Red=Cloning sites Green=Tags MASGKHHQPGGTRSLTMQKVSLRVTPRLVLEVNRHNAICVATNVPEFYNARGDLNIRDLRAHVKARMISS QFCGYVLVSLLDSEDQVDHLNIFPHVFSERMILYKPNNVNLMEMCALLSMIENAKSPSIGLCREVLGRLT LLHSKCNNLDSLFLYNGARTLLSTLVKYHDLEEGAATPGPWNEGLSLFKLHKELKRAPSEARDLMQSLFL TSGKMGCLARSPKDYCADLNKEEDANSGFTFNLFYQDSLLTKHFQCQTVLQTLRRKCLGSDTVSKIIP TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_009334 |
| ORF Size | 834 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_009334.1, YP_001129477 |
| RefSeq ORF | 834 bp |
| Locus ID | 5176152 |
| MW | 31.3 kDa |
Documents
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Resources
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| VC101034 | Myc-DDK-tagged ORF clone of viral ORF for BSLF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129457 |
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| VC101035 | Myc-DDK-tagged ORF clone of viral ORF for BSRF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129458 |
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| VC101036 | Myc-DDK-tagged ORF clone of viral ORF for BLLF3 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129459 |
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| VC101037 | Myc-DDK-tagged ORF clone of viral ORF for BLRF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129460 |
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| VC101038 | Myc-DDK-tagged ORF clone of viral ORF for BLRF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129461 |
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| VC101040 | Myc-DDK-tagged ORF clone of viral ORF for EBNA-3A [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129463 |
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| VC101043 | Myc-DDK-tagged ORF clone of viral ORF for BZLF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129466 |
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| VC101044 | Myc-DDK-tagged ORF clone of viral ORF for BRLF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129468 |
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| VC101045 | Myc-DDK-tagged ORF clone of viral ORF for BZLF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129467 |
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| VC101046 | Myc-DDK-tagged ORF clone of viral ORF for BRRF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129469 |
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| VC101047 | Myc-DDK-tagged ORF clone of viral ORF for BRRF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129470 |
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| VC101049 | Myc-DDK-tagged ORF clone of viral ORF for BKRF2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129472 |
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| VC101050 | Myc-DDK-tagged ORF clone of viral ORF for BKRF3 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129473 |
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| VC101051 | Myc-DDK-tagged ORF clone of viral ORF for BKRF4 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129474 |
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| VC101052 | Myc-DDK-tagged ORF clone of viral ORF for BBLF4 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129475 |
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| VC101057 | Myc-DDK-tagged ORF clone of viral ORF for BBLF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129480 |
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| VC101058 | Myc-DDK-tagged ORF clone of viral ORF for BGLF5 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129481 |
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| VC101076 | Myc-DDK-tagged ORF clone of viral ORF for BVRF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129499 |
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| VC101084 | Myc-DDK-tagged ORF clone of viral ORF for BALF4 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129508 |
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| VC101085 | Myc-DDK-tagged ORF clone of viral ORF for BALF3 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129509 |
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| VC101087 | Myc-DDK-tagged ORF clone of viral ORF for BALF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129511 |
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| VC101088 | Myc-DDK-tagged ORF clone of viral ORF for BARF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129512 |
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| VC101089 | Myc-DDK-tagged ORF clone of viral ORF for BNLF2b [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129513 |
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| VC101090 | Myc-DDK-tagged ORF clone of viral ORF for BNLF2a [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129514 |
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| VC101092 | Myc-DDK-tagged ORF clone of viral ORF for BVLF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129500 |
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